FastQCFastQC Report
Thu 26 May 2016
SRR1511812_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511812_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025242
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49910.16497853725420975No Hit
GTCCTACAGTGGACATTTCTAAATT42950.14197211330531573No Hit
CTGTAGGACGTGGAATATGGCAAGA38410.12696504940761766No Hit
GTCCTAAAGTGTGTATTTCTCATTT31460.10399168066554676No Hit
GGTATCAACGCAGAGTACTTTTTTT30940.10227280991074433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG457.0686155E-414.692425
TCCAACG4450.012.52316818
TAGGACC9350.012.3241234
GGTATCA13500.012.2874381
ACTTCGC550.003205667212.0210728
CCAACGA2850.011.26824419
CGTTAGG802.3621712E-411.2418961
CGTGCGG600.00613674511.01931610
GTAGGAC24250.010.7888673
GGCGAGG10700.010.769567519
TCGGTCA2500.010.57854315
GGACGTG23850.010.4945876
TGGCGAG23800.010.47729418
AGGACGT23650.010.4635245
TGTAGGA24250.010.3018512
CTGTAGG24600.010.155281
TAGGACG25150.010.0647884
CGGTCAG2450.010.02373216
CCAACGT2450.010.02373219
CGGCACT856.957498E-410.0007244
Produced by FastQC (version 0.11.5)