FastQCFastQC Report
Thu 26 May 2016
SRR1511812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025242
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63260.2091072383630797No Hit
GTCCTACAGTGGACATTTCTAAATT48620.16071441557402683No Hit
CTGTAGGACGTGGAATATGGCAAGA42360.14002185610275145No Hit
GGTATCAACGCAGAGTACTTTTTTT39560.13076639819227684No Hit
TATCAACGCAGAGTACTTTTTTTTT38950.12875003057606632No Hit
GTCCTAAAGTGTGTATTTCTCATTT36570.12088289135216292No Hit
CTTTAGGACGTGAAATATGGCGAGG34170.11294964171461325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA602.5774685E-514.2455256
TCCAACG4400.013.81452718
TAAGACG500.001499933413.300224
GCCGGTT2900.012.7718511
CCGGTTT2900.012.44436612
TAGGACC9950.012.3167874
TATACCG951.0406329E-612.0001995
CCAACGA2700.011.95979919
CGGTTTC3100.011.94786113
GGTATCA16300.011.9401071
CCAACGT2600.011.32392819
TATATCG600.00587711511.0835175
TAATTCG600.00587711511.0835174
TGTAGGA26400.010.9035892
GTAGGAC26650.010.7300083
GTCCTAC25400.010.7047561
AAGGCGT1701.4006218E-1010.6143126
CTAGGAC4700.010.5108123
TGCCGGT3300.010.36038110
TAGGACT4800.010.2918374
Produced by FastQC (version 0.11.5)