FastQCFastQC Report
Thu 26 May 2016
SRR1511811_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511811_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324943
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49130.2113170086320396No Hit
GTCCTACAGTGGACATTTCTAAATT48900.21032773706710228No Hit
CTGTAGGACGTGGAATATGGCAAGA47580.20465017852050568No Hit
GTCCTAAAGTGTGTATTTCTCATTT45020.19363915588468192No Hit
CTTTAGGACGTGAAATATGGCGAGG40030.1721762641062598No Hit
GGTATCAACGCAGAGTACTTTTTTT32040.13780983017648174No Hit
TATCAACGCAGAGTACTTTTTTTTT30600.13161612994383087No Hit
GTCCTACAGTGTGCATTTCTCATTT28510.1226266622450529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGC250.006238234718.8902328
GGTATCA11900.013.9400261
TAGGACC9750.012.690364
CGTTAGG655.245501E-412.2992621
GTATTAG2150.011.1551461
GGCGAGG12900.011.12936919
GTCCTAA15300.011.1035011
CTACACT3600.011.0193014
GCACCGT700.00156574310.7944196
CGTAGGA650.00731462410.7618542
GTATCAA24300.010.6922611
TGTAGGA28300.010.6287572
GTCCTAT3050.010.4846171
TCCTATA3650.010.4038292
CGGTAGG1353.220539E-710.3632681
GTAGGAC29150.010.3361643
AGGACGT29300.010.3154865
AGGACCT18150.010.2517235
TACACCG1201.6420399E-610.2322095
GTCTTAG3050.010.1569731
Produced by FastQC (version 0.11.5)