##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511811_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2324943 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.026763666894198 31.0 30.0 34.0 22.0 34.0 2 26.323939124529076 31.0 25.0 34.0 7.0 34.0 3 28.06340241459683 31.0 27.0 34.0 16.0 34.0 4 32.38278916945491 35.0 32.0 37.0 24.0 37.0 5 32.74225819729774 35.0 33.0 37.0 26.0 37.0 6 32.93701652040502 36.0 35.0 37.0 26.0 37.0 7 32.90893497173909 37.0 35.0 37.0 26.0 37.0 8 32.92078042343404 37.0 35.0 37.0 26.0 37.0 9 34.42384436951787 39.0 35.0 39.0 27.0 39.0 10 34.288566644429565 39.0 35.0 39.0 26.0 39.0 11 34.27584332175025 39.0 35.0 39.0 26.0 39.0 12 34.15643996433461 39.0 35.0 39.0 25.0 39.0 13 34.14396309931039 39.0 35.0 39.0 25.0 39.0 14 35.17737166029447 40.0 35.0 41.0 25.0 41.0 15 35.11508712256602 40.0 35.0 41.0 25.0 41.0 16 35.06271852686281 39.0 35.0 41.0 25.0 41.0 17 35.01125360922827 39.0 35.0 41.0 24.0 41.0 18 34.97157306652249 39.0 35.0 41.0 24.0 41.0 19 34.99209227925158 40.0 35.0 41.0 24.0 41.0 20 34.92434610224853 40.0 35.0 41.0 23.0 41.0 21 34.80526232256017 39.0 35.0 41.0 22.0 41.0 22 34.69353872331494 39.0 35.0 41.0 19.0 41.0 23 34.57583992381749 39.0 34.0 41.0 18.0 41.0 24 34.45224807661951 39.0 34.0 41.0 18.0 41.0 25 34.336692555473405 39.0 34.0 41.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.143177370599684 1101 2 3.5968823250629924 1101 3 2.097106544687726 1101 4 1.381186876161813 1101 5 0.7311389686863379 1101 6 0.9521371306366788 1101 7 0.6110967931584241 1101 8 0.693685099119584 1101 9 0.7114765545022053 1101 10 0.6294574179565444 1101 11 0.5646748787332001 1101 12 0.65257586643407 1101 13 0.6525296184261364 1101 14 0.6358570796363949 1101 15 0.7939947666614202 1101 16 0.6732504183213237 1101 17 0.7910849406163294 1101 18 0.8416445926703986 1101 19 0.9697251464056862 1101 20 0.8165733692880082 1101 21 0.7659970049695062 1101 22 0.9281105150352715 1101 23 0.8433007010001461 1101 24 0.8250414810367275 1101 25 1.0445390352942283 1102 1 1.71628973436 1102 2 4.338268483758807 1102 3 2.7134522082584525 1102 4 1.8653184212304268 1102 5 1.6327491957125702 1102 6 1.4315867349816997 1102 7 1.3193685034753173 1102 8 1.2379311291888015 1102 9 1.2724220698959883 1102 10 1.3738578224852347 1102 11 1.3082611551818033 1102 12 1.3388731612188636 1102 13 1.5498846881340924 1102 14 1.7894836117606516 1102 15 1.5759168977050493 1102 16 1.5872077850536286 1102 17 1.7282365064747793 1102 18 1.7661053384567964 1102 19 1.807613653114494 1102 20 1.8522161219987225 1102 21 1.6954664695323984 1102 22 1.8750203496461424 1102 23 2.0048177962813014 1102 24 1.7114001875590503 1102 25 1.7492344978682866 1103 1 1.2606076072326005 1103 2 3.778432150769845 1103 3 2.2253448006496583 1103 4 1.4954318825248976 1103 5 1.2359513960641948 1103 6 0.9155784519447039 1103 7 0.9398966603636509 1103 8 0.8521088066759788 1103 9 0.8484230123859717 1103 10 1.1250585909770692 1103 11 1.2021336109119574 1103 12 1.167649917965825 1103 13 1.048673497877985 1103 14 1.2824887954555706 1103 15 0.9694032950370826 1103 16 1.1246862618448148 1103 17 1.1408368725958908 1103 18 1.1768361306921307 1103 19 1.300776069513823 1103 20 1.2673779765290192 1103 21 1.2772850220852803 1103 22 1.284930541225357 1103 23 1.4624125437121052 1103 24 1.6104386665252122 1103 25 1.528200205732638 1104 1 1.2163488963112385 1104 2 3.793514439710762 1104 3 2.1255410546091653 1104 4 1.5509510947546161 1104 5 1.23730107241731 1104 6 0.9849490620535022 1104 7 0.7070361728274932 1104 8 0.8547702858110853 1104 9 1.0468340439243278 1104 10 1.0406607807580883 1104 11 0.941365466028337 1104 12 1.0826392445048683 1104 13 0.9643049536003403 1104 14 1.1821892499384248 1104 15 1.107311296689744 1104 16 1.0933062866347854 1104 17 0.9748318457400842 1104 18 1.0743364749973168 1104 19 0.8834093156231759 1104 20 0.8992021054368777 1104 21 1.001964818256603 1104 22 0.9273764853101909 1104 23 1.0367652499509177 1104 24 1.1014281996473727 1104 25 1.1643901244766113 1105 1 1.2777386406550484 1105 2 3.8625789088862987 1105 3 2.307719220902996 1105 4 1.5737927048896694 1105 5 1.1078410498817632 1105 6 0.9618687139090554 1105 7 0.8103742093271507 1105 8 0.8011318855044394 1105 9 0.8849818582737186 1105 10 0.7699205194514889 1105 11 0.8841495064337082 1105 12 0.6727328975433622 1105 13 0.7586585931571648 1105 14 0.982125145891608 1105 15 0.8724463777945815 1105 16 0.6033225439497514 1105 17 0.8781200281260126 1105 18 0.8205257537607054 1105 19 0.9143188661348489 1105 20 0.9915763673316533 1105 21 1.175462503571822 1105 22 0.8144216026635505 1105 23 0.952052908600173 1105 24 1.0592727671598396 1105 25 0.9758639227735628 1106 1 1.1245832952033652 1106 2 3.811165762523146 1106 3 2.129508049214053 1106 4 1.2853211360113619 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0.9419468475266228 1107 18 0.8789406299664364 1107 19 1.1161788271335595 1107 20 1.0783787681901842 1107 21 0.8437895081652371 1107 22 1.1036500292844664 1107 23 1.1095378240921079 1107 24 1.1351681822924604 1107 25 1.1761748725752312 1108 1 1.2652749780457277 1108 2 3.7438593596292904 1108 3 2.282295046300753 1108 4 1.4418327339019825 1108 5 1.1399550288198057 1108 6 0.9837410297608926 1108 7 0.9158980686565741 1108 8 0.7854473410911353 1108 9 0.91915292420763 1108 10 0.8841764874995448 1108 11 0.896143965065626 1108 12 0.742291043319895 1108 13 0.8587247885464464 1108 14 0.9796670085238901 1108 15 0.9568458830953972 1108 16 0.8589077799205711 1108 17 0.859064663976497 1108 18 0.8991663753830252 1108 19 0.8807487038217587 1108 20 0.9510677840089699 1108 21 0.8892711151926491 1108 22 0.9662958652127429 1108 23 1.0391224993828843 1108 24 1.0150852330110425 1108 25 1.0587565614546293 1109 1 0.48421607816091594 1109 2 2.4420748698875627 1109 3 1.3779196989262488 1109 4 0.8354197856873569 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2314 13 -0.5740314763930243 2314 14 -1.3278731256421992 2314 15 -1.0628419879817912 2314 16 -0.8796293470745695 2314 17 -0.9265181874384112 2314 18 -0.8335069224811136 2314 19 -0.7779855085307759 2314 20 -0.6245722807608658 2314 21 -0.6739482540725277 2314 22 -0.5736646560302177 2314 23 -0.7836474905932107 2314 24 -0.762587062462778 2314 25 -0.6491188337184752 2315 1 -4.49699459115919 2315 2 -13.456288476005685 2315 3 -6.901612669136213 2315 4 -3.4365063616506717 2315 5 -2.4497833042707953 2315 6 -1.969661679023318 2315 7 -1.6327630772875956 2315 8 -1.5395266771284213 2315 9 -1.7562764283860801 2315 10 -1.6691448359277814 2315 11 -1.70927295399769 2315 12 -1.8067948092643817 2315 13 -1.6915745778413367 2315 14 -1.944096371590561 2315 15 -1.89288063180242 2315 16 -1.975148652413779 2315 17 -1.9533631150226753 2315 18 -1.945886201969401 2315 19 -1.8668461455241143 2315 20 -1.918083300546769 2315 21 -2.0181876734209467 2315 22 -2.0098123377324058 2315 23 -2.1105699709564476 2315 24 -2.105083864239205 2315 25 -2.195742901593732 2316 1 -2.0413692477423986 2316 2 -1.8510175985155648 2316 3 -1.533381179780001 2316 4 -1.7482150584789338 2316 5 -1.946977583204042 2316 6 -2.0989155288187753 2316 7 -2.0594127443891495 2316 8 -2.1614445597150507 2316 9 -2.406211208590598 2316 10 -2.4881002737483264 2316 11 -2.3734850038638555 2316 12 -2.245057590953497 2316 13 -2.2621240944274064 2316 14 -2.649873728798717 2316 15 -3.1607542622169547 2316 16 -2.821567624057444 2316 17 -2.6662116498919204 2316 18 -2.7479129808532647 2316 19 -2.757373371681261 2316 20 -2.830128960719314 2316 21 -2.9123350114360633 2316 22 -2.874916004755214 2316 23 -2.8068526643357963 2316 24 -2.8126288142973834 2316 25 -2.7447127083289367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 105712.0 3 2.0 4 916.0 5 4755.0 6 5610.0 7 5074.0 8 4620.0 9 4527.0 10 4273.0 11 4014.0 12 4056.0 13 4005.0 14 4063.0 15 4126.0 16 4305.0 17 4768.0 18 5391.0 19 6740.0 20 8345.0 21 10716.0 22 13430.0 23 15896.0 24 18430.0 25 20653.0 26 23468.0 27 26152.0 28 29885.0 29 36400.0 30 44319.0 31 56356.0 32 71070.0 33 92059.0 34 123219.0 35 173250.0 36 259879.0 37 348346.0 38 776113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53011794267644 19.394238912523875 12.930596578066645 25.145046566733033 2 15.84860049385233 21.309509615375863 40.538623056696544 22.303266834075263 3 18.553530129555863 25.067066160331674 29.88998009843682 26.48942361167564 4 12.058360140442153 17.18437828368265 39.06319423745012 31.694067338425068 5 12.706720833055549 38.267263385858094 36.171979001563486 12.854036779522868 6 30.852336805558544 37.85359189926905 18.5938391157396 12.700232179432808 7 27.536804128101206 31.72778859524728 22.750450226091566 17.98495705055995 8 24.29801504811086 37.88910093709824 20.867565355365702 16.945318659425197 9 26.2181051320398 15.271858277815845 20.711131412684097 37.79890517746026 10 14.708532639294813 29.138047685470138 35.2139815900863 20.939438085148755 11 33.71093398848918 22.35732230854692 25.61796138658023 18.31378231638367 12 23.369648202127966 24.693766685892946 32.45468813644033 19.481896975538753 13 28.897593530330447 22.38422394374739 26.039843591729856 22.678338934192308 14 20.415812344646728 22.799268627230862 28.0934629365107 28.691456091611705 15 22.252717593506592 32.203671229789286 23.541007241897972 22.002603934806146 16 21.405643063077246 28.426460347630027 28.801179211705406 21.36671737758732 17 21.18374367561332 29.16944990578207 28.362457520208146 21.28434889839647 18 20.954277158622816 27.609192999570315 30.84875629208974 20.587773549717134 19 23.15861597910652 27.063626697681144 27.965543997770258 21.812213325442077 20 23.26626186647099 27.66200144262222 28.702375509315754 20.36936118159104 21 22.537008688874963 26.98213750367216 27.548649914599473 22.93220389285341 22 22.80063040680509 28.707124078649443 27.750115276353547 20.742130238191915 23 21.652008934427958 28.346980286364502 28.404401147729036 21.596609631478504 24 22.785609396680982 27.044964398560627 29.086724366490518 21.08270183826787 25 22.218346358815104 27.8436770139316 28.637034233288745 21.30094239396455 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 195.0 1 195.0 2 371.5 3 548.0 4 548.0 5 548.0 6 1389.0 7 2230.0 8 2230.0 9 2230.0 10 3332.5 11 4435.0 12 4435.0 13 4435.0 14 7507.5 15 10580.0 16 10580.0 17 10580.0 18 19659.0 19 28738.0 20 28738.0 21 28738.0 22 46129.5 23 63521.0 24 63521.0 25 63521.0 26 92916.5 27 122312.0 28 122312.0 29 122312.0 30 158070.5 31 193829.0 32 193829.0 33 193829.0 34 217938.5 35 242048.0 36 242048.0 37 242048.0 38 257938.0 39 273828.0 40 273828.0 41 273828.0 42 285290.0 43 296752.0 44 296752.0 45 296752.0 46 299169.5 47 301587.0 48 301587.0 49 301587.0 50 286466.0 51 271345.0 52 271345.0 53 271345.0 54 237990.5 55 204636.0 56 204636.0 57 204636.0 58 173282.0 59 141928.0 60 141928.0 61 141928.0 62 114539.0 63 87150.0 64 87150.0 65 87150.0 66 66650.0 67 46150.0 68 46150.0 69 46150.0 70 33481.5 71 20813.0 72 20813.0 73 20813.0 74 14471.0 75 8129.0 76 8129.0 77 8129.0 78 5497.5 79 2866.0 80 2866.0 81 2866.0 82 1899.0 83 932.0 84 932.0 85 932.0 86 607.5 87 283.0 88 283.0 89 283.0 90 181.0 91 79.0 92 79.0 93 79.0 94 52.0 95 25.0 96 25.0 97 25.0 98 14.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.485080709505566 3 0.0 4 0.0 5 3.440944573694925E-4 6 7.312007219101716E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 8.602361434237313E-5 14 0.0 15 0.0 16 0.0 17 6.021653003966119E-4 18 0.0 19 3.0108265019830594E-4 20 4.3011807171186567E-4 21 6.88188914738985E-4 22 0.004086121681262723 23 1.7204722868474626E-4 24 0.0015914368653339027 25 5.591534932254253E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2324943.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.96452946768453 #Duplication Level Percentage of deduplicated Percentage of total 1 86.12090163334258 64.56012868276271 2 9.908941491303047 14.856382728366983 3 2.182905607868877 4.909214752987807 4 0.719913921156457 2.1587203342691814 5 0.3394364779236873 1.2722847925858651 6 0.1759532104739208 0.7914149778903559 7 0.11086780325268322 0.5817806892767255 8 0.07997581605877364 0.47962795357120436 9 0.057649404584404304 0.3889494439885839 >10 0.2680895310911637 3.524385514341895 >50 0.017471127623119476 0.8969685974371447 >100 0.014760618953646188 2.230906267377655 >500 0.001682728419701175 0.9306017021777506 >1k 0.001450627948014709 2.418633562966308 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4913 0.2113170086320396 No Hit GTCCTACAGTGGACATTTCTAAATT 4890 0.21032773706710228 No Hit CTGTAGGACGTGGAATATGGCAAGA 4758 0.20465017852050568 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4502 0.19363915588468192 No Hit CTTTAGGACGTGAAATATGGCGAGG 4003 0.1721762641062598 No Hit GGTATCAACGCAGAGTACTTTTTTT 3204 0.13780983017648174 No Hit TATCAACGCAGAGTACTTTTTTTTT 3060 0.13161612994383087 No Hit GTCCTACAGTGTGCATTTCTCATTT 2851 0.1226266622450529 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.3011807171186564E-5 0.0 7 0.0 0.0 0.0 8.602361434237313E-5 0.0 8 4.3011807171186564E-5 0.0 0.0 8.602361434237313E-5 0.0 9 4.3011807171186564E-5 0.0 0.0 8.602361434237313E-5 0.0 10 4.3011807171186564E-5 0.0 0.0 8.602361434237313E-5 0.0 11 4.3011807171186564E-5 0.0 0.0 8.602361434237313E-5 0.0 12 4.3011807171186564E-5 0.0 0.0 8.602361434237313E-5 4.3011807171186564E-5 13 4.3011807171186564E-5 0.0 0.0 8.602361434237313E-5 4.3011807171186564E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCGC 25 0.0062382347 18.890232 8 GGTATCA 1190 0.0 13.940026 1 TAGGACC 975 0.0 12.69036 4 CGTTAGG 65 5.245501E-4 12.299262 1 GTATTAG 215 0.0 11.155146 1 GGCGAGG 1290 0.0 11.129369 19 GTCCTAA 1530 0.0 11.103501 1 CTACACT 360 0.0 11.019301 4 GCACCGT 70 0.001565743 10.794419 6 CGTAGGA 65 0.007314624 10.761854 2 GTATCAA 2430 0.0 10.692261 1 TGTAGGA 2830 0.0 10.628757 2 GTCCTAT 305 0.0 10.484617 1 TCCTATA 365 0.0 10.403829 2 CGGTAGG 135 3.220539E-7 10.363268 1 GTAGGAC 2915 0.0 10.336164 3 AGGACGT 2930 0.0 10.315486 5 AGGACCT 1815 0.0 10.251723 5 TACACCG 120 1.6420399E-6 10.232209 5 GTCTTAG 305 0.0 10.156973 1 >>END_MODULE