FastQCFastQC Report
Thu 26 May 2016
SRR1511811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324943
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59920.2577267485697499No Hit
GTCCTACAGTGGACATTTCTAAATT55350.23807035269251764No Hit
CTGTAGGACGTGGAATATGGCAAGA52490.22576897584155828No Hit
GTCCTAAAGTGTGTATTTCTCATTT48590.2089943710447955No Hit
CTTTAGGACGTGAAATATGGCGAGG47250.20323078888385648No Hit
GGTATCAACGCAGAGTACTTTTTTT37080.15948778099075978No Hit
TATCAACGCAGAGTACTTTTTTTTT36780.15819742677562418No Hit
GTCCTACAGTGTGCATTTCTCATTT33110.14241209354379872No Hit
CTGAAGGACCTGGAATATGGCGAGA25160.10821770684270539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGA601.4722427E-615.82851210
TTGGCGT456.7631825E-414.7780474
GGTATCA12350.013.516451
AATCCCG2000.012.34703619
TAGGACC9700.011.948674
ACACCGT907.4902473E-611.6075766
GGCGAGG13750.011.32819119
TCCAACG1201.280423E-711.08067318
GATATAC5200.010.8228151
AGGACCT18950.010.778565
AATGTCC8300.010.6413378
GGACCTG18300.010.586946
GTCTAGG2350.010.5535131
GTCCTAC27950.010.5113971
GTATCAA27050.010.4027051
TGGCGAG30550.010.3837618
ACTGTTC6200.010.2630038
TGGACTG3800.010.2501865
CCAACGA750.00265369310.13090219
ATTTAGA9700.010.12877751
Produced by FastQC (version 0.11.5)