FastQCFastQC Report
Thu 26 May 2016
SRR1511810_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511810_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815017
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53610.29536913428359074No Hit
GGTATCAACGCAGAGTACTTTTTTT32220.17751899844464267No Hit
TATCAACGCAGAGTACTTTTTTTTT31220.17200940817634214No Hit
GTCCTACAGTGGACATTTCTAAATT22310.12291895888578454No Hit
CTGTAGGACGTGGAATATGGCAAGA20560.11327717591625863No Hit
GTCCTAAAGTGTGTATTTCTCATTT18880.10402106426551376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.014.3132831
TAGGACC4950.011.8307384
ATTAGAC1451.07320375E-1011.725463
TAGGACA5250.011.6944414
GCCTACG709.809899E-411.415321
CGTGCGC752.1879746E-411.33461210
GTATCAA21750.011.1594611
GGATAAG1103.3194356E-610.8964431
GCGTGCG803.9771534E-410.6261999
GCCGGTT901.00874575E-410.4950111
ATCGTGC901.00874575E-410.495018
GTTACAC2350.010.4503513
TCGAACT1002.553824E-510.3909219
TATGTCG1002.5546002E-510.39063316
GTCCTAC15150.010.2191611
TCCTACA15750.010.1469522
ACTATTC1401.0537951E-710.1201898
GGCGAGG6000.010.07604419
CTATGCG750.002777316110.0752119
GTAGGAC14850.010.0497683
Produced by FastQC (version 0.11.5)