##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511810_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1815017 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.816355439095062 31.0 28.0 34.0 20.0 34.0 2 26.143398105913057 31.0 25.0 34.0 2.0 34.0 3 27.852879614901678 31.0 27.0 34.0 16.0 34.0 4 32.14839365140933 35.0 32.0 37.0 20.0 37.0 5 32.48369960171172 35.0 33.0 37.0 25.0 37.0 6 32.67709283163739 35.0 33.0 37.0 25.0 37.0 7 32.62957151365524 37.0 33.0 37.0 25.0 37.0 8 32.64958675318193 37.0 33.0 37.0 25.0 37.0 9 34.13048803399638 39.0 35.0 39.0 25.0 39.0 10 33.99509756657927 38.0 35.0 39.0 25.0 39.0 11 33.989414424217514 39.0 35.0 39.0 25.0 39.0 12 33.83897946961378 38.0 34.0 39.0 23.0 39.0 13 33.826033585360356 38.0 34.0 39.0 23.0 39.0 14 34.84489291284875 39.0 35.0 41.0 22.0 41.0 15 34.75896975069655 39.0 34.0 41.0 22.0 41.0 16 34.70093888927762 39.0 34.0 41.0 21.0 41.0 17 34.64202649341576 39.0 34.0 41.0 19.0 41.0 18 34.60346266729182 39.0 34.0 41.0 19.0 41.0 19 34.620411819834196 39.0 34.0 41.0 18.0 41.0 20 34.54169740558904 39.0 34.0 41.0 18.0 41.0 21 34.41939441889525 39.0 34.0 41.0 18.0 41.0 22 34.30105723527659 39.0 34.0 41.0 17.0 41.0 23 34.1626877324014 39.0 34.0 41.0 11.0 41.0 24 34.02469839125474 39.0 34.0 41.0 8.0 41.0 25 33.89304232412148 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9080830284149215 1101 2 3.478346517382242 1101 3 2.1569098603435606 1101 4 1.1909773059127744 1101 5 0.5446020252085617 1101 6 0.6006796152329557 1101 7 0.44520196669832046 1101 8 0.5006061659419387 1101 9 0.5578939991491154 1101 10 0.5882205292300569 1101 11 0.5410117088876163 1101 12 0.3898053912540007 1101 13 0.314106594616149 1101 14 0.32962454600097857 1101 15 0.543852376264887 1101 16 0.43247747944986514 1101 17 0.4440733175346452 1101 18 0.5371848978913079 1101 19 0.6846565773451516 1101 20 0.46089317932889884 1101 21 0.6493370784044004 1101 22 0.5308348127272708 1101 23 0.4858566499079444 1101 24 0.6818667760343331 1101 25 0.8660191112104627 1102 1 1.6567008438472897 1102 2 4.368668655974123 1102 3 2.659867108376229 1102 4 1.9394543163241238 1102 5 1.5444947941341596 1102 6 1.5579344997282973 1102 7 1.324679720532771 1102 8 1.24368496536502 1102 9 1.2671155289562677 1102 10 1.3313329025997547 1102 11 1.392405541559178 1102 12 1.4154377291312343 1102 13 1.4827968338476438 1102 14 1.5907544806003031 1102 15 1.4255902048527602 1102 16 1.5685057500832826 1102 17 1.665678152182963 1102 18 1.5052207877550643 1102 19 1.552377968402837 1102 20 1.5408430239637454 1102 21 1.6254296876824554 1102 22 1.5985760740798867 1102 23 1.861109910520561 1102 24 1.4088612568886205 1102 25 1.4713388687928628 1103 1 1.169448493043138 1103 2 3.6652181150744276 1103 3 2.151025255220013 1103 4 1.4885155947102007 1103 5 1.0959359301330025 1103 6 1.0393798632166025 1103 7 1.0865753874402913 1103 8 0.8294078407989218 1103 9 0.6268684818829229 1103 10 0.8972323894061134 1103 11 1.1282255145099782 1103 12 1.0148697970809692 1103 13 1.1454094897047398 1103 14 1.204809956714989 1103 15 1.0309976859932704 1103 16 1.021126372484261 1103 17 1.0949428216556 1103 18 1.1799998551136213 1103 19 1.0221281759877243 1103 20 1.2280933536573997 1103 21 1.3665732656880536 1103 22 1.4179659345053608 1103 23 1.3812581926608516 1103 24 1.45150062508948 1103 25 1.4169935654325982 1104 1 1.3035245014023538 1104 2 3.911904090128612 1104 3 2.2439071289865815 1104 4 1.6263960920756517 1104 5 1.4283649097738973 1104 6 1.0674031961234434 1104 7 0.960740338452041 1104 8 1.053552824301498 1104 9 1.2653489738203092 1104 10 1.3093375464515375 1104 11 1.243475845040109 1104 12 1.3200460345818925 1104 13 1.2979787071722413 1104 14 1.5220636293796588 1104 15 1.492463301167028 1104 16 1.5018635419056636 1104 17 1.494473294400919 1104 18 1.4294415402938512 1104 19 1.5016944351621149 1104 20 1.4413957858163329 1104 21 1.4523356562060101 1104 22 1.5860816995323717 1104 23 1.576766278532773 1104 24 1.6390779264903017 1104 25 1.6216382406988643 1105 1 1.6545317088508753 1105 2 4.224894858840447 1105 3 2.622981470370629 1105 4 2.0490313495447623 1105 5 1.4102954444544693 1105 6 1.4089943408329404 1105 7 1.2521286031583614 1105 8 1.2436461788653048 1105 9 1.331735130360336 1105 10 1.2998718797997526 1105 11 1.420353477147728 1105 12 1.015801675786868 1105 13 1.3650183952628367 1105 14 1.4877116796978669 1105 15 1.6077018711854336 1105 16 1.6135889148030813 1105 17 1.5653138823064978 1105 18 1.559294663108183 1105 19 1.5604242277681593 1105 20 1.5230041430835541 1105 21 1.4338705996823506 1105 22 1.590828793983178 1105 23 1.5689648231539763 1105 24 1.675732737821825 1105 25 1.7128375144975791 1106 1 0.8646781644499981 1106 2 3.621261724409461 1106 3 1.9472274439551747 1106 4 1.0767009071853764 1106 5 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1107 18 0.6310818437472605 1107 19 0.7596477530141286 1107 20 0.749609333312037 1107 21 0.7702511578240134 1107 22 0.880958098046456 1107 23 1.0985984094508225 1107 24 1.0609491860309461 1107 25 1.1108338194075458 1108 1 0.8526145164246692 1108 2 3.385993294594236 1108 3 1.8006623084438331 1108 4 1.1478182047826593 1108 5 0.9006609827167935 1108 6 0.5489612301812343 1108 7 0.4833125410407817 1108 8 0.5025138707481958 1108 9 0.46559880540464604 1108 10 0.6225519621122189 1108 11 0.45494738369285415 1108 12 0.5094830399171499 1108 13 0.4593605442179225 1108 14 0.6317382539117915 1108 15 0.5252323069201807 1108 16 0.40640067801074053 1108 17 0.3951620874639943 1108 18 0.5173752331541124 1108 19 0.4559060877250971 1108 20 0.3803923886831768 1108 21 0.40414986220268645 1108 22 0.32233645930137556 1108 23 0.23093506165302102 1108 24 0.42884563895522376 1108 25 0.3256526578005321 1109 1 0.6221085087447484 1109 2 2.6337713820462376 1109 3 1.4717186072175217 1109 4 0.8618547050510443 1109 5 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-0.8365003441081384 2314 14 -1.5145895710286723 2314 15 -1.206985016636672 2314 16 -1.1406637707070075 2314 17 -1.1339057590514372 2314 18 -0.9645703187013908 2314 19 -0.9589243611281475 2314 20 -1.0315337769646717 2314 21 -0.9779004377102822 2314 22 -0.9019843720125564 2314 23 -1.045687621252199 2314 24 -1.1530920958810569 2314 25 -1.1424882986899405 2315 1 -4.52992505389857 2315 2 -13.256435518099687 2315 3 -6.849058440937572 2315 4 -3.3936737502335106 2315 5 -2.2035404757229102 2315 6 -1.6487440183688342 2315 7 -1.5271840354225716 2315 8 -1.5204336437511046 2315 9 -1.583697242145206 2315 10 -1.567001734390935 2315 11 -1.555278451898836 2315 12 -1.4951517610202245 2315 13 -1.6411013386617768 2315 14 -1.6584967460227489 2315 15 -1.8073757341360803 2315 16 -1.7531738346425954 2315 17 -1.6047748094895127 2315 18 -1.562545691659004 2315 19 -1.4699970582628836 2315 20 -1.7657985176703264 2315 21 -1.9421604424462515 2315 22 -1.6820980352851151 2315 23 -1.9462014739635407 2315 24 -2.0162628275883776 2315 25 -1.9823509555689363 2316 1 -2.1655935816681833 2316 2 -1.948487139218308 2316 3 -1.4720371851828489 2316 4 -1.8921613592059856 2316 5 -2.1609251363165036 2316 6 -2.3511771655918743 2316 7 -2.1842287998461174 2316 8 -2.4649776735287503 2316 9 -2.431589787938133 2316 10 -2.7606774127139815 2316 11 -2.7533301709702442 2316 12 -2.4566479898379363 2316 13 -2.5368707578017506 2316 14 -2.795646809692066 2316 15 -3.317851784679714 2316 16 -2.8172653225454276 2316 17 -2.793099816835973 2316 18 -2.7629521957240435 2316 19 -2.9440415288280697 2316 20 -3.1102739804972472 2316 21 -3.0307038845071794 2316 22 -3.1251693449138713 2316 23 -3.1399462090012555 2316 24 -3.049377236443309 2316 25 -3.324559301146369 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91549.0 3 5.0 4 832.0 5 3999.0 6 4834.0 7 4194.0 8 3926.0 9 3699.0 10 3590.0 11 3466.0 12 3414.0 13 3392.0 14 3338.0 15 3401.0 16 3706.0 17 4030.0 18 4375.0 19 5540.0 20 7119.0 21 8893.0 22 10920.0 23 13062.0 24 14989.0 25 17126.0 26 19096.0 27 21290.0 28 24487.0 29 29011.0 30 35869.0 31 44806.0 32 56359.0 33 73598.0 34 98336.0 35 137288.0 36 201430.0 37 273650.0 38 576398.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.474404371970074 18.10545025198111 12.227929545563484 25.19221583048533 2 16.848989437009504 20.71499734905644 38.82851599596824 23.607497217965822 3 19.239103545586627 24.21475942098614 29.266557833893568 27.279579199533664 4 12.63613508854187 15.995111891513963 37.44190825760861 33.92684476233556 5 13.44453596546566 37.69912479579946 35.20954487728684 13.64679436144804 6 32.693371474663415 36.403170461332316 17.488060632373262 13.415397431631003 7 28.573120802725267 31.41254324339662 21.63483868195174 18.37949727192638 8 25.909399195710016 36.23866883891446 19.97656220299865 17.87536976237688 9 26.905312732608017 14.86933731199212 19.472324501643786 38.75302545375608 10 15.403657376211905 28.401111394548924 34.0505350638589 22.14469616538027 11 35.228871134540334 21.954890780637314 23.919445382605232 18.896792702217112 12 23.644131156898254 24.139002554797006 31.17260058721213 21.044265701092606 13 29.41647378509402 21.350819303620845 25.548135361817547 23.68457154946758 14 21.560514309232364 21.283877781860998 27.032033308778924 30.12357460012771 15 23.477466051282164 30.039608444438816 23.208542950286414 23.274382553992606 16 22.99647882085953 27.57368112805555 26.75032795836072 22.6795120927242 17 22.337875280921782 27.978624875826924 27.148159759163466 22.535340084087828 18 22.262876876635314 26.96062901890175 28.864302648404944 21.912191456057986 19 23.85616395713304 26.379729930011443 27.252947778277925 22.511158334577587 20 24.040418510090852 26.53566647015719 27.67566018920006 21.748254830551897 21 23.622673694023476 26.078208178282996 26.9485653459335 23.35055278176002 22 23.880962967738167 26.847356731468032 27.03887916400128 22.232801136792528 23 23.16297054174387 26.821299508707668 27.323060106038042 22.69266984351042 24 23.66769000885966 26.11368354703774 27.71696250292016 22.501663941182436 25 23.312736398722862 26.43395711833917 27.662665737371178 22.59064074556679 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 274.0 1 274.0 2 418.5 3 563.0 4 563.0 5 563.0 6 1424.5 7 2286.0 8 2286.0 9 2286.0 10 3139.5 11 3993.0 12 3993.0 13 3993.0 14 5834.0 15 7675.0 16 7675.0 17 7675.0 18 13449.5 19 19224.0 20 19224.0 21 19224.0 22 29844.5 23 40465.0 24 40465.0 25 40465.0 26 57812.0 27 75159.0 28 75159.0 29 75159.0 30 98071.0 31 120983.0 32 120983.0 33 120983.0 34 137920.0 35 154857.0 36 154857.0 37 154857.0 38 170455.5 39 186054.0 40 186054.0 41 186054.0 42 199512.5 43 212971.0 44 212971.0 45 212971.0 46 223301.0 47 233631.0 48 233631.0 49 233631.0 50 230979.0 51 228327.0 52 228327.0 53 228327.0 54 209447.5 55 190568.0 56 190568.0 57 190568.0 58 166928.0 59 143288.0 60 143288.0 61 143288.0 62 119652.0 63 96016.0 64 96016.0 65 96016.0 66 75100.0 67 54184.0 68 54184.0 69 54184.0 70 40603.0 71 27022.0 72 27022.0 73 27022.0 74 19052.0 75 11082.0 76 11082.0 77 11082.0 78 7755.5 79 4429.0 80 4429.0 81 4429.0 82 2912.0 83 1395.0 84 1395.0 85 1395.0 86 907.5 87 420.0 88 420.0 89 420.0 90 268.5 91 117.0 92 117.0 93 117.0 94 71.0 95 25.0 96 25.0 97 25.0 98 17.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.4350455119704115 3 0.0 4 0.0 5 6.611508321960621E-4 6 7.162467348790672E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 5.509590268300518E-4 18 0.0 19 3.3057541609803103E-4 20 3.856713187810362E-4 21 8.264385402450777E-4 22 0.004738247630738445 23 4.407672214640414E-4 24 0.002699699231467253 25 0.0012121098590261138 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1815017.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.32843431833346 #Duplication Level Percentage of deduplicated Percentage of total 1 84.6787727147009 62.09361823167032 2 10.210086208132056 14.973792717950673 3 2.5526650807178157 5.615488011243591 4 0.9786668690544756 2.8705643690796068 5 0.48527897465373065 1.7792373709482148 6 0.2776347413494147 1.221511253731683 7 0.19147641321255124 0.9828465912836646 8 0.12307761701942689 0.7220071164532836 9 0.08636283995123205 0.5699566653217797 >10 0.3873553250577382 4.72053222939621 >50 0.016089300577178046 0.8154842204090292 >100 0.010331525416604214 1.5477967847952856 >500 0.0012151118107045003 0.5691406001618189 >1k 9.113338580283727E-4 1.219475622538501 >5k 7.594448816903107E-5 0.2985482150163898 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5361 0.29536913428359074 No Hit GGTATCAACGCAGAGTACTTTTTTT 3222 0.17751899844464267 No Hit TATCAACGCAGAGTACTTTTTTTTT 3122 0.17200940817634214 No Hit GTCCTACAGTGGACATTTCTAAATT 2231 0.12291895888578454 No Hit CTGTAGGACGTGGAATATGGCAAGA 2056 0.11327717591625863 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1888 0.10402106426551376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 970 0.0 14.313283 1 TAGGACC 495 0.0 11.830738 4 ATTAGAC 145 1.07320375E-10 11.72546 3 TAGGACA 525 0.0 11.694441 4 GCCTACG 70 9.809899E-4 11.41532 1 CGTGCGC 75 2.1879746E-4 11.334612 10 GTATCAA 2175 0.0 11.159461 1 GGATAAG 110 3.3194356E-6 10.896443 1 GCGTGCG 80 3.9771534E-4 10.626199 9 GCCGGTT 90 1.00874575E-4 10.49501 11 ATCGTGC 90 1.00874575E-4 10.49501 8 GTTACAC 235 0.0 10.450351 3 TCGAACT 100 2.553824E-5 10.39092 19 TATGTCG 100 2.5546002E-5 10.390633 16 GTCCTAC 1515 0.0 10.219161 1 TCCTACA 1575 0.0 10.146952 2 ACTATTC 140 1.0537951E-7 10.120189 8 GGCGAGG 600 0.0 10.076044 19 CTATGCG 75 0.0027773161 10.075211 9 GTAGGAC 1485 0.0 10.049768 3 >>END_MODULE