FastQCFastQC Report
Thu 26 May 2016
SRR1511810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815017
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61100.3366359653931616No Hit
GGTATCAACGCAGAGTACTTTTTTT38600.21267018435639998No Hit
TATCAACGCAGAGTACTTTTTTTTT38180.21035615644371375No Hit
GTCCTACAGTGGACATTTCTAAATT24510.13504005747604567No Hit
CTGTAGGACGTGGAATATGGCAAGA23240.12804287783530402No Hit
GTCCTAAAGTGTGTATTTCTCATTT19950.10991632585259532No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18970.10451692738966081No Hit
CTTTAGGACGTGAAATATGGCGAGG18460.10170703635282756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.013.8255361
TAGGACC4900.012.99043754
TAGGACT3150.012.3656884
CCGGTTT1001.4487523E-712.34618712
GCCGGTT1001.93716E-611.3964811
TGTAGGA14550.011.2960892
GTGGTAT3650.011.2375471
GTAGGAC14400.011.1498563
AGCGTAC600.005700458311.1286561
TCCAACG1201.2804776E-711.08021618
TATGTCG951.3650975E-510.99660216
CGGTTTC1158.852858E-710.73581313
ATAGGAC1951.8189894E-1210.7184773
AGGACGT14450.010.7167925
ACCGTGT1252.249817E-710.6367148
TAGGACG15500.010.5424554
TGGTATC3700.010.5275462
TTAGGAC9400.010.5111573
GATATAC4450.010.5034491
GTCCTAC14450.010.430021
Produced by FastQC (version 0.11.5)