FastQCFastQC Report
Thu 26 May 2016
SRR1511809_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511809_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012659
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51420.2554829208524643No Hit
GTCCTACAGTGGACATTTCTAAATT46190.22949739623055868No Hit
CTGTAGGACGTGGAATATGGCAAGA44170.2194609220936085No Hit
GTCCTAAAGTGTGTATTTCTCATTT40530.20137539444088642No Hit
CTTTAGGACGTGAAATATGGCGAGG35410.17593641048980477No Hit
GGTATCAACGCAGAGTACTTTTTTT33140.1646577984646182No Hit
TATCAACGCAGAGTACTTTTTTTTT30410.15109365272507663No Hit
GTCCTACAGTGTGCATTTCTCATTT23300.1157672511836332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTCG400.005488902314.1668077
TAGGACC10000.013.6008114
GGTATCA8350.013.5265661
CGTGCGC604.3001416E-412.59271710
GTCCTAG2000.011.4945881
GGCGAGG13100.011.46464319
TCCAACG1251.99434E-811.33485418
GTATCAA19250.011.21551
GTCCTAC23900.010.9990051
GGACCTG17600.010.9476226
AGGACCT18500.010.8745225
AACCGTG700.001566384110.7937577
TGGCGAG26550.010.74427518
CGGTAGG750.001735606310.6616471
GTAGGAC27950.010.6446423
TGTAGGA26800.010.6293242
GTCCTAA13250.010.4856291
TCCTACA26450.010.1653472
CTGTAGG26550.010.1647061
GAGACGA750.00277876510.0746756
Produced by FastQC (version 0.11.5)