##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511809_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2012659 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.74896840448382 31.0 28.0 34.0 16.0 34.0 2 26.065110383825576 31.0 25.0 34.0 2.0 34.0 3 27.785573711194992 31.0 27.0 34.0 16.0 34.0 4 32.08078218913388 35.0 32.0 37.0 19.0 37.0 5 32.429749401165324 35.0 33.0 37.0 24.0 37.0 6 32.62011796335097 36.0 33.0 37.0 25.0 37.0 7 32.57952837514949 37.0 33.0 37.0 24.0 37.0 8 32.591865288655455 37.0 33.0 37.0 24.0 37.0 9 34.06881891070469 39.0 35.0 39.0 25.0 39.0 10 33.921756243854524 38.0 34.0 39.0 23.0 39.0 11 33.919005156859654 39.0 35.0 39.0 23.0 39.0 12 33.78307800774995 38.0 34.0 39.0 22.0 39.0 13 33.7702497044954 38.0 34.0 39.0 22.0 39.0 14 34.78724165395131 39.0 34.0 41.0 22.0 41.0 15 34.72080317629564 39.0 34.0 41.0 19.0 41.0 16 34.67090103191847 39.0 34.0 41.0 19.0 41.0 17 34.61449356299303 39.0 34.0 41.0 19.0 41.0 18 34.58049426157138 39.0 34.0 41.0 18.0 41.0 19 34.59912732360524 39.0 34.0 41.0 18.0 41.0 20 34.533643304702885 39.0 34.0 41.0 18.0 41.0 21 34.41212247082094 39.0 34.0 41.0 18.0 41.0 22 34.29927970908137 39.0 34.0 41.0 16.0 41.0 23 34.170837186031015 39.0 34.0 41.0 10.0 41.0 24 34.04787149735748 39.0 34.0 41.0 2.0 41.0 25 33.9302797940436 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1271653355568496 1101 2 3.5953396930025647 1101 3 2.2392917227871685 1101 4 1.4551842435285636 1101 5 0.8225154442767533 1101 6 0.9733426536550667 1101 7 0.6757066140575105 1101 8 0.77307960450905 1101 9 0.8475973589894679 1101 10 0.7479434460500798 1101 11 0.7780749698267613 1101 12 0.7438319547966898 1101 13 0.6256815033672041 1101 14 0.6377590017166384 1101 15 0.875952557384089 1101 16 0.8468210408591759 1101 17 0.9391350636713724 1101 18 1.0448768595070916 1101 19 1.0216113382926792 1101 20 0.8392808446536293 1101 21 0.9316570111226525 1101 22 0.9458438208181619 1101 23 0.7297719598764161 1101 24 0.875741202989758 1101 25 0.982489990506167 1102 1 1.4953975490506366 1102 2 3.987299403765551 1102 3 2.391144868990974 1102 4 1.6942089549584267 1102 5 1.2055765416283464 1102 6 1.273665284948784 1102 7 1.219367863017105 1102 8 1.0424333458333734 1102 9 1.0749469032368921 1102 10 1.022249603057226 1102 11 1.0708931735926512 1102 12 1.1077600541083825 1102 13 1.2458403034691798 1102 14 1.45789889302484 1102 15 1.31134205321149 1102 16 1.3719280359310702 1102 17 1.5563363258333567 1102 18 1.3511385403300267 1102 19 1.5133041311721627 1102 20 1.4938090786904752 1102 21 1.3491213081122737 1102 22 1.6534652465074515 1102 23 1.6211828091345026 1102 24 1.4320082019109464 1102 25 1.4837702171415685 1103 1 1.6285628229758409 1103 2 3.9745873668738945 1103 3 2.4418767124689325 1103 4 1.818212374298632 1103 5 1.4658592768679028 1103 6 1.3076028180227581 1103 7 1.2510985260085619 1103 8 1.1860950637610657 1103 9 1.170702967041315 1103 10 1.4073004570199927 1103 11 1.388031162578656 1103 12 1.205795678050741 1103 13 1.2581278304371892 1103 14 1.5721531221322635 1103 15 1.2796055750156015 1103 16 1.2737208634579176 1103 17 1.3854042067147034 1103 18 1.456882217749012 1103 19 1.3405896518946463 1103 20 1.405103950261683 1103 21 1.6669164151992675 1103 22 1.3899841488294626 1103 23 1.5729311503474364 1103 24 1.663482414386884 1103 25 1.6036622869472836 1104 1 1.811203787030184 1104 2 4.2647572588105085 1104 3 2.6800489522636717 1104 4 1.9237275950112576 1104 5 1.7712429009823722 1104 6 1.5731110947082527 1104 7 1.4204487192455417 1104 8 1.5347905396424082 1104 9 1.5953408487731195 1104 10 1.6860797771730276 1104 11 1.604366743615877 1104 12 1.7268735925845746 1104 13 1.6965150679951506 1104 14 1.8868793156217478 1104 15 1.8357805693553715 1104 16 1.8219420938496924 1104 17 1.7002782035789679 1104 18 1.8128024561282317 1104 19 1.894146068169647 1104 20 1.8915360860538328 1104 21 1.6948315256815292 1104 22 1.7374191988064283 1104 23 1.83805368146961 1104 24 1.822231736828293 1104 25 2.043202208729859 1105 1 1.5765993299393521 1105 2 4.155142848396835 1105 3 2.543251053948815 1105 4 1.8833723291683455 1105 5 1.496883088718448 1105 6 1.2294356286858275 1105 7 1.0734394965465412 1105 8 1.0353951594112019 1105 9 1.2583851242925164 1105 10 1.301691153785363 1105 11 1.2753505600123916 1105 12 0.9449399794641664 1105 13 1.2717403555712607 1105 14 1.329889086288631 1105 15 1.3224168599552968 1105 16 1.268441449559333 1105 17 1.2519802574596213 1105 18 1.3089194472708456 1105 19 1.2848925459776623 1105 20 1.4200188571730692 1105 21 1.384291277868762 1105 22 1.3144833181835125 1105 23 1.4280952214305955 1105 24 1.3683664941170477 1105 25 1.5568553136671355 1106 1 1.076033068249334 1106 2 3.713702169790036 1106 3 2.307034498543622 1106 4 1.325661266274743 1106 5 1.0894045030874366 1106 6 0.9076266153722514 1106 7 0.8078982970394648 1106 8 0.6443313585780643 1106 9 0.7363553520824269 1106 10 0.9550134995754433 1106 11 0.8303852992976459 1106 12 0.5496651270777804 1106 13 0.6160939931789002 1106 14 0.7238499696266274 1106 15 0.6762903469630288 1106 16 0.9905967216900322 1106 17 0.7199936541981344 1106 18 0.7829689365768928 1106 19 0.7311173420402923 1106 20 0.9160111759731109 1106 21 0.7359087903998542 1106 22 0.7172896778541897 1106 23 0.6462337400779177 1106 24 0.7873714158308047 1106 25 0.8411746278025092 1107 1 1.199151753213897 1107 2 3.605987366178951 1107 3 2.0884299642853428 1107 4 1.468831048137119 1107 5 1.1453493965024961 1107 6 1.0489985434226128 1107 7 0.9746637273859378 1107 8 1.085307344810289 1107 9 1.002559544511925 1107 10 1.0365860894016024 1107 11 0.8989417823644033 1107 12 0.845182320533354 1107 13 0.9972354529297434 1107 14 1.2152525025897418 1107 15 1.1785885245661447 1107 16 1.0609081821209898 1107 17 1.1617278143076675 1107 18 1.1228330921622174 1107 19 1.0601884607607417 1107 20 1.1885507331924714 1107 21 1.1817951648561333 1107 22 1.2583478685863696 1107 23 1.184101331693057 1107 24 1.307193345507514 1107 25 1.2372409354898082 1108 1 0.9574382884104473 1108 2 3.5365082019130085 1108 3 1.9408482307952895 1108 4 1.0960594973070457 1108 5 0.8508484016419757 1108 6 0.7605747763710653 1108 7 0.5852486848899048 1108 8 0.494712810013219 1108 9 0.492803765486201 1108 10 0.4270320993322798 1108 11 0.6600510582544672 1108 12 0.41064900871052146 1108 13 0.4813097460922222 1108 14 0.6403757398172445 1108 15 0.6823139379390142 1108 16 0.6238117920434689 1108 17 0.6714608024487205 1108 18 0.6102727539352415 1108 19 0.4693442505417167 1108 20 0.5346564368034166 1108 21 0.426446093008515 1108 22 0.5568070511362322 1108 23 0.7109133990823722 1108 24 0.7015025384285138 1108 25 0.7545179625341376 1109 1 0.8992806159371938 1109 2 3.022356895520364 1109 3 1.7291598494880276 1109 4 1.384197073264609 1109 5 1.0949673415656633 1109 6 0.9896297874557121 1109 7 0.8000819862578226 1109 8 0.8955276302107578 1109 9 0.9181165599501853 1109 10 0.9890557600907073 1109 11 1.0239993147894069 1109 12 0.9715998228755254 1109 13 1.010486861080743 1109 14 1.0277069535062253 1109 15 0.9871000535934513 1109 16 0.8911066485505401 1109 17 1.0524588142397917 1109 18 1.0113784792383882 1109 19 1.0837003341155835 1109 20 1.0053885479858735 1109 21 0.9563391538138717 1109 22 1.0432442259973627 1109 23 0.8630815474749483 1109 24 0.7702011728899265 1109 25 0.8321896257322621 1110 1 0.7925633397341514 1110 2 3.32514974525402 1110 3 1.8512849434598984 1110 4 0.9107395646675513 1110 5 0.7442676612714862 1110 6 0.5961687329675698 1110 7 0.352640726591531 1110 8 0.31800811917531746 1110 9 0.3190053352477591 1110 10 0.49936014260485706 1110 11 0.3841538603800103 1110 12 0.34392759675819917 1110 13 0.42355346972373553 1110 14 0.5352040954949828 1110 15 0.6040579323216733 1110 16 0.7216130547553874 1110 17 0.7010934734831125 1110 18 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-1.1973436283143144 2314 11 -1.1418384271375501 2314 12 -1.073080456618328 2314 13 -1.2706138915392629 2314 14 -2.171929261090085 2314 15 -1.7726844207145476 2314 16 -1.5212483668714825 2314 17 -1.6267634849928356 2314 18 -1.4733172479201926 2314 19 -1.542951779031064 2314 20 -1.3683707769243583 2314 21 -1.354140958501219 2314 22 -1.367723024243368 2314 23 -1.3951703891044147 2314 24 -1.4854712877029428 2314 25 -1.4448208093309418 2315 1 -4.698737060460161 2315 2 -13.351065102803226 2315 3 -7.06877373628441 2315 4 -3.6691302372401218 2315 5 -2.7264911657504953 2315 6 -2.1171529569473755 2315 7 -2.0034923091021035 2315 8 -1.7835143927187005 2315 9 -1.8165088257706898 2315 10 -1.994509713278923 2315 11 -1.8685846562022235 2315 12 -1.9522586054054756 2315 13 -1.9057569083403685 2315 14 -1.9800755778724266 2315 15 -2.0405010828456156 2315 16 -2.0507398025086374 2315 17 -1.9352176062704487 2315 18 -2.0064993651395397 2315 19 -1.9613178655809307 2315 20 -2.0295160078041476 2315 21 -2.3588237481346823 2315 22 -2.3050557463712096 2315 23 -2.238939792016538 2315 24 -2.236062787543098 2315 25 -2.2165566945793103 2316 1 -1.8229699903260048 2316 2 -1.7396219460240303 2316 3 -1.3388098534444524 2316 4 -1.648888718254085 2316 5 -1.8057159247220191 2316 6 -1.9247519532052877 2316 7 -2.015921781892253 2316 8 -2.1458809702757193 2316 9 -2.21555197273965 2316 10 -2.3433946379143613 2316 11 -2.4075003152758505 2316 12 -2.345182456026553 2316 13 -2.329637056939635 2316 14 -2.6379556825837938 2316 15 -3.195210609488793 2316 16 -2.855342642830262 2316 17 -2.867948266228293 2316 18 -2.9322713726624414 2316 19 -2.875526863633084 2316 20 -2.960427901554123 2316 21 -2.992112429832524 2316 22 -2.9073677996666056 2316 23 -2.930289422390061 2316 24 -2.912429702004289 2316 25 -3.0766620771720774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104571.0 3 0.0 4 906.0 5 4595.0 6 5482.0 7 4803.0 8 4343.0 9 4152.0 10 4093.0 11 3782.0 12 3743.0 13 3682.0 14 3721.0 15 3877.0 16 3895.0 17 4308.0 18 4974.0 19 6085.0 20 7836.0 21 9950.0 22 12184.0 23 14594.0 24 16785.0 25 18891.0 26 21172.0 27 23386.0 28 27107.0 29 31888.0 30 39558.0 31 49377.0 32 62096.0 33 80123.0 34 106547.0 35 149966.0 36 222059.0 37 298138.0 38 649990.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.72979178291007 19.371339109108895 12.721777509255169 25.177091598725866 2 15.827885501678123 21.23105834547517 40.65427612501032 22.286780027836393 3 18.611150721508213 24.93094955479294 29.957682846423562 26.500216877275285 4 11.899979082397962 16.932873377954238 39.32926541455855 31.837882125089244 5 12.598073484248868 38.33256568830432 36.385778146114895 12.683582681331917 6 31.024612400404646 38.00880831383991 18.36946822190114 12.597111063854314 7 27.395847980209265 31.859147525735853 22.832134007797645 17.912870486257233 8 24.154563689129656 38.18292120026294 20.937724671690535 16.724790438916877 9 26.200066677961843 15.161137579689358 20.85584294209799 37.78295280025081 10 14.542950395471859 29.28469253857708 35.542931018120804 20.629426047830258 11 33.74580592142037 22.310386409222822 25.61462224847826 18.32918542087855 12 23.100733904749884 24.833665315386263 32.63831578026879 19.427284999595063 13 28.867874092741136 22.434832380694964 26.0123935734671 22.684899953096803 14 20.037472815812315 23.056712537990787 28.32372498272186 28.582089663475035 15 22.21593424420133 32.364548589701485 23.636145020095306 21.78337214600188 16 21.117685608938224 28.63286826034614 29.09240959347808 21.157036537237556 17 20.998416513178913 29.409826959223352 28.54191519923762 21.049841328360113 18 20.705593943136915 27.682980574453993 31.24985404879813 20.36157143361096 19 22.915834431387044 27.232576719671357 28.355026104860652 21.496562744080943 20 23.1358044688324 27.68969881017623 29.062167260990602 20.112329460000765 21 22.17678726253264 27.168079815367342 27.8926487284146 22.762484193685424 22 22.485798058906568 29.00763548425725 28.092738811980862 20.413827644855328 23 21.302409007008155 28.610964124502214 28.812936146532813 21.273690721956818 24 22.567878979999264 27.097433899790623 29.601972746054162 20.732714374155954 25 21.998812507918682 27.998628670232456 29.03035557686527 20.972203244983593 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 170.0 1 170.0 2 367.5 3 565.0 4 565.0 5 565.0 6 1506.5 7 2448.0 8 2448.0 9 2448.0 10 3725.5 11 5003.0 12 5003.0 13 5003.0 14 8025.0 15 11047.0 16 11047.0 17 11047.0 18 19204.5 19 27362.0 20 27362.0 21 27362.0 22 42812.5 23 58263.0 24 58263.0 25 58263.0 26 83303.5 27 108344.0 28 108344.0 29 108344.0 30 139800.0 31 171256.0 32 171256.0 33 171256.0 34 191858.0 35 212460.0 36 212460.0 37 212460.0 38 224942.0 39 237424.0 40 237424.0 41 237424.0 42 246556.5 43 255689.0 44 255689.0 45 255689.0 46 257907.0 47 260125.0 48 260125.0 49 260125.0 50 245842.0 51 231559.0 52 231559.0 53 231559.0 54 201999.0 55 172439.0 56 172439.0 57 172439.0 58 145748.0 59 119057.0 60 119057.0 61 119057.0 62 95998.0 63 72939.0 64 72939.0 65 72939.0 66 55726.5 67 38514.0 68 38514.0 69 38514.0 70 27965.5 71 17417.0 72 17417.0 73 17417.0 74 12178.5 75 6940.0 76 6940.0 77 6940.0 78 4708.5 79 2477.0 80 2477.0 81 2477.0 82 1643.5 83 810.0 84 810.0 85 810.0 86 537.5 87 265.0 88 265.0 89 265.0 90 164.0 91 63.0 92 63.0 93 63.0 94 40.5 95 18.0 96 18.0 97 18.0 98 11.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.5067450571607015 3 0.0 4 0.0 5 4.968551552945631E-4 6 7.452827329418446E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.4905654658836892E-4 14 0.0 15 0.0 16 0.0 17 5.962261863534757E-4 18 0.0 19 3.4779860870619414E-4 20 3.9748412423565044E-4 21 6.955972174123883E-4 22 0.004074212273415417 23 1.9874206211782522E-4 24 0.0016396220124720582 25 6.955972174123883E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2012659.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.96969846392831 #Duplication Level Percentage of deduplicated Percentage of total 1 87.5828649962679 67.41226709369681 2 9.098958922935793 14.006882492680756 3 1.8572360945705155 4.2885270652624925 4 0.5781425885547723 1.7799784284086313 5 0.2684185299558586 1.0330046656416672 6 0.14762962356466813 0.681780456606945 7 0.09269737073799784 0.4994422071871865 8 0.06060583942318354 0.3731850548444556 9 0.04741392910512415 0.32844922435903323 >10 0.23228630836478267 3.176443561136477 >50 0.01675288916545266 0.8969664723953354 >100 0.013922610231055987 2.150638265122982 >500 0.0016331367674869055 0.891529180722599 >1k 0.0013718348846889867 2.222361866716928 >5k 6.532547069947556E-5 0.2585439652177299 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5142 0.2554829208524643 No Hit GTCCTACAGTGGACATTTCTAAATT 4619 0.22949739623055868 No Hit CTGTAGGACGTGGAATATGGCAAGA 4417 0.2194609220936085 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4053 0.20137539444088642 No Hit CTTTAGGACGTGAAATATGGCGAGG 3541 0.17593641048980477 No Hit GGTATCAACGCAGAGTACTTTTTTT 3314 0.1646577984646182 No Hit TATCAACGCAGAGTACTTTTTTTTT 3041 0.15109365272507663 No Hit GTCCTACAGTGTGCATTTCTCATTT 2330 0.1157672511836332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.9685515529456306E-5 4.9685515529456306E-5 10 0.0 0.0 0.0 4.9685515529456306E-5 4.9685515529456306E-5 11 0.0 0.0 0.0 4.9685515529456306E-5 4.9685515529456306E-5 12 0.0 0.0 0.0 4.9685515529456306E-5 4.9685515529456306E-5 13 0.0 0.0 0.0 4.9685515529456306E-5 4.9685515529456306E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCTCG 40 0.0054889023 14.166807 7 TAGGACC 1000 0.0 13.600811 4 GGTATCA 835 0.0 13.526566 1 CGTGCGC 60 4.3001416E-4 12.592717 10 GTCCTAG 200 0.0 11.494588 1 GGCGAGG 1310 0.0 11.464643 19 TCCAACG 125 1.99434E-8 11.334854 18 GTATCAA 1925 0.0 11.2155 1 GTCCTAC 2390 0.0 10.999005 1 GGACCTG 1760 0.0 10.947622 6 AGGACCT 1850 0.0 10.874522 5 AACCGTG 70 0.0015663841 10.793757 7 TGGCGAG 2655 0.0 10.744275 18 CGGTAGG 75 0.0017356063 10.661647 1 GTAGGAC 2795 0.0 10.644642 3 TGTAGGA 2680 0.0 10.629324 2 GTCCTAA 1325 0.0 10.485629 1 TCCTACA 2645 0.0 10.165347 2 CTGTAGG 2655 0.0 10.164706 1 GAGACGA 75 0.002778765 10.074675 6 >>END_MODULE