FastQCFastQC Report
Thu 26 May 2016
SRR1511809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511809_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012659
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65120.32355207712781947No Hit
GTCCTACAGTGGACATTTCTAAATT51640.25657600219411236No Hit
CTGTAGGACGTGGAATATGGCAAGA49660.24673827011928No Hit
GTCCTAAAGTGTGTATTTCTCATTT43850.2178709855966659No Hit
GGTATCAACGCAGAGTACTTTTTTT41940.20838105213053976No Hit
CTTTAGGACGTGAAATATGGCGAGG40860.20301501645335848No Hit
TATCAACGCAGAGTACTTTTTTTTT40790.2026672178446523No Hit
GTCCTACAGTGTGCATTTCTCATTT28800.14309428472483415No Hit
CTGAAGGACCTGGAATATGGCGAGA22360.11109681272386429No Hit
CTGTAGGACCTGGAATATGGCGAGA20820.10344524333232802No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20540.10205404889750325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1250.014.436110518
GGTATCA12750.013.4677081
TAGGACC8750.013.1368684
CGAAATC1457.2759576E-1212.44461313
GCGCCAC1201.0062649E-811.87150813
GGCGAGG11300.011.59864419
ACGAAAT1604.5474735E-1111.27793112
AGGACCT17650.010.8722935
AGTGCGG700.001495399310.85394910
AATGTCC6750.010.833858
ACCTTTT7550.010.69221815
CGTGCGC909.545789E-510.5524510
AAATGTC6600.010.5044847
GTCCTAC26000.010.4202071
TAGAAAT6850.010.4012424
GGACCTG18050.010.365376
AGGACGT27450.010.3476895
GAAATGT7000.010.3112516
CGCCACC1201.5298283E-610.2886414
GGACGTG27400.010.2597556
Produced by FastQC (version 0.11.5)