FastQCFastQC Report
Thu 26 May 2016
SRR1511808_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511808_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350245
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT70530.3000963729313327No Hit
CTGTAGGACGTGGAATATGGCAAGA64430.2741416320426168No Hit
GTATCAACGCAGAGTACTTTTTTTT60720.258356043731611No Hit
GTCCTAAAGTGTGTATTTCTCATTT60510.2574625198649502No Hit
CTTTAGGACGTGAAATATGGCGAGG55730.2371242147095303No Hit
GGTATCAACGCAGAGTACTTTTTTT38630.16436584271001534No Hit
TATCAACGCAGAGTACTTTTTTTTT36910.15704745675450857No Hit
GTCCTACAGTGTGCATTTCTCATTT33650.14317656244348995No Hit
CTGAAGGACCTGGAATATGGCGAGA29510.12556137764360736No Hit
CTGTAGGACCTGGAATATGGCGAGA27040.11505183502145522No Hit
GTCCTTCAGTGTGCATTTCTCATTT24340.10356367102153179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.013.7685341
TAGGACC10600.013.4550384
CGATAAT450.00824388313.3226951
TCCAACG1550.012.79761918
CCAACGT803.0404983E-511.80732619
CCAACGA1002.0681327E-611.33503319
ATAGGAC4850.011.1006143
GGCGAGG16800.010.79527119
TCGGTCA1502.3483153E-910.70439815
CCACCTT9800.010.60159513
TGTAGGA37450.010.5923432
AATGCCG2600.010.53576918
CGGTAGG959.869315E-510.5179181
GTATTAG2400.010.4083551
GTATTAT2900.010.3365741
GTATCAA26100.010.298291
AGGACCT21200.010.2917685
TATAGGA5150.010.2830512
GCCTTAG1751.0750227E-910.2775081
TAGGACA9850.010.2603324
Produced by FastQC (version 0.11.5)