FastQCFastQC Report
Thu 26 May 2016
SRR1511808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511808_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350245
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78130.33243342715333934No Hit
GTATCAACGCAGAGTACTTTTTTTT72410.3080955389757238No Hit
CTGTAGGACGTGGAATATGGCAAGA72100.30677652755351037No Hit
GTCCTAAAGTGTGTATTTCTCATTT66250.2818855055536763No Hit
CTTTAGGACGTGAAATATGGCGAGG63280.2692485251537606No Hit
GGTATCAACGCAGAGTACTTTTTTT47000.1999791511097779No Hit
TATCAACGCAGAGTACTTTTTTTTT45560.19385213030981877No Hit
GTCCTACAGTGTGCATTTCTCATTT40910.1740669589766173No Hit
CTGAAGGACCTGGAATATGGCGAGA34480.1467081091545775No Hit
CTGTAGGACCTGGAATATGGCGAGA30400.12934821688802658No Hit
TTTCTAAATTTTCCACCTTTTTCAG26500.11275420222147053No Hit
GTCCTTCAGTGTGCATTTCTCATTT26340.11207342213258617No Hit
ATTTAGAAATGTCCACTGTAGGACG25590.10888226546594078No Hit
GAATATGGCAAGAAAACTGAAAATC24650.10488268244374523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCG456.7793333E-414.7734099
GGTATCA16250.014.0871621
TCCAACG2450.012.79349618
TAGGACC11150.012.3540254
CGTGCGC701.0943751E-412.21067410
CCAACGA1052.7286478E-711.75967819
GCGTGCG658.046036E-411.6888519
GTATTAG2500.011.0642921
GTGCGCC951.3653716E-510.99674911
TAGAACG700.001492131610.85693554
CCAACGT1752.0008883E-1110.85508819
GGCGAGG16300.010.66362619
ATAGGAC3850.010.6101883
TGTAGGA42300.010.5104382
AGGACCT23600.010.425655
ACCTTTT9300.010.41627515
GGCGAAA1106.0619896E-610.36167419
CCACCTT9550.010.34249213
GGACCTG23750.010.1969846
CTGTAGG42350.010.0224111
Produced by FastQC (version 0.11.5)