FastQCFastQC Report
Thu 26 May 2016
SRR1511807_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511807_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062716
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT63390.30731327046476586No Hit
CTGTAGGACGTGGAATATGGCAAGA58880.2854488935946587No Hit
GTCCTAAAGTGTGTATTTCTCATTT53500.25936677661878804No Hit
GTATCAACGCAGAGTACTTTTTTTT49460.23978094900121974No Hit
CTTTAGGACGTGAAATATGGCGAGG46160.22378262446211694No Hit
GTCCTACAGTGTGCATTTCTCATTT31640.15338999649006455No Hit
GGTATCAACGCAGAGTACTTTTTTT30750.14907529684163986No Hit
TATCAACGCAGAGTACTTTTTTTTT29260.14185181091337828No Hit
CTGAAGGACCTGGAATATGGCGAGA26470.1283259547121368No Hit
CTGTAGGACCTGGAATATGGCGAGA23290.11290938742900138No Hit
GTCCTTCAGTGTGCATTTCTCATTT21450.10398910950416829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9600.013.7739764
GGTATCA10200.012.7367471
CGTGCGC604.298403E-412.59334910
TCCAACG2200.012.45084618
CCAACGA1600.012.39717919
GATATAC6450.011.9301611
TGTAGGA34550.011.5119892
GGACCTG20250.011.4739416
AGGACCT21050.011.4417015
CTGTAGG33700.011.3278851
ACTGTTC7100.011.3074088
TTAGGAC19850.011.134173
TATACTG3000.011.0191795
GTCCTAA15600.010.8902951
AGGACGT35650.010.8889195
TACGACT700.001565758510.7942984
GTAGGAC35900.010.7867813
GGCGAGG14550.010.71135719
GGACGTG35300.010.6758066
GTCGAGG1951.8189894E-1210.65642619
Produced by FastQC (version 0.11.5)