##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511807_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2062716 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.47753980674024 31.0 30.0 34.0 25.0 34.0 2 26.72817052856525 31.0 26.0 34.0 10.0 34.0 3 28.475467296515856 31.0 28.0 34.0 16.0 34.0 4 32.84044580058525 35.0 33.0 37.0 27.0 37.0 5 33.21859577372746 35.0 35.0 37.0 27.0 37.0 6 33.42326573314019 37.0 35.0 37.0 28.0 37.0 7 33.406513063359185 37.0 35.0 37.0 28.0 37.0 8 33.42183945826764 37.0 35.0 37.0 28.0 37.0 9 34.97209116524039 39.0 35.0 39.0 28.0 39.0 10 34.84189728493889 39.0 35.0 39.0 27.0 39.0 11 34.84023539837767 39.0 35.0 39.0 27.0 39.0 12 34.73243335485835 39.0 35.0 39.0 27.0 39.0 13 34.72717426926441 39.0 35.0 39.0 27.0 39.0 14 35.8314154735795 40.0 36.0 41.0 27.0 41.0 15 35.790808817112975 40.0 36.0 41.0 27.0 41.0 16 35.74535030513168 40.0 36.0 41.0 27.0 41.0 17 35.70509367261417 40.0 36.0 41.0 27.0 41.0 18 35.678388105778986 40.0 36.0 41.0 27.0 41.0 19 35.700945258581406 40.0 36.0 41.0 27.0 41.0 20 35.63705667673107 40.0 36.0 41.0 27.0 41.0 21 35.5286263353753 40.0 36.0 41.0 26.0 41.0 22 35.43288315017676 40.0 36.0 41.0 26.0 41.0 23 35.32442323616048 40.0 36.0 41.0 25.0 41.0 24 35.22397605874973 40.0 36.0 41.0 25.0 41.0 25 35.135336614444256 40.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9497266957417168 1101 2 3.600288463232644 1101 3 2.210201901793546 1101 4 1.2708433350311878 1101 5 0.6389476726485626 1101 6 0.8396645911617213 1101 7 0.6394198785535465 1101 8 0.6602547203443407 1101 9 0.8194646886126407 1101 10 0.8044032755974015 1101 11 0.6737766178201454 1101 12 0.5278380520350012 1101 13 0.5581500652177525 1101 14 0.6655333259219987 1101 15 0.7847781574318446 1101 16 0.7035327653935965 1101 17 0.8088097865694408 1101 18 0.9164939038427207 1101 19 0.9297275352292758 1101 20 0.8136239225160438 1101 21 0.958890192927953 1101 22 0.8603874346809519 1101 23 0.6227692875303603 1101 24 0.8328479015409727 1101 25 0.8735134271609439 1102 1 1.3249406290951846 1102 2 3.9070814764210056 1102 3 2.3498122914039357 1102 4 1.4212888197789937 1102 5 1.1347696800984792 1102 6 0.9913810917154322 1102 7 0.9615280029872579 1102 8 1.0124393574127026 1102 9 1.0013332076904646 1102 10 1.0451663884535378 1102 11 0.9658082296191992 1102 12 1.0236978124296456 1102 13 1.052449370563565 1102 14 1.264219523445405 1102 15 1.2280606501329387 1102 16 1.2580606018981797 1102 17 1.3651120114763131 1102 18 1.2379098909563808 1102 19 1.3199671405851632 1102 20 1.363998935100362 1102 21 1.2654818570777593 1102 22 1.4365854614603748 1102 23 1.3416105238134577 1102 24 1.1933885237095012 1102 25 1.3602747278757334 1103 1 1.6577337196370507 1103 2 4.211431490191902 1103 3 2.7422845554096433 1103 4 1.932043358877742 1103 5 1.5795625164084441 1103 6 1.3336866925854736 1103 7 1.392811238511925 1103 8 1.1166832646745561 1103 9 1.223606040893145 1103 10 1.3562261823844608 1103 11 1.4939591162529595 1103 12 1.3830325369764438 1103 13 1.3868658377288128 1103 14 1.7156029687113943 1103 15 1.464807929498491 1103 16 1.4185736206901538 1103 17 1.3878322222063133 1103 18 1.687244474263231 1103 19 1.6099804171400365 1103 20 1.7289126374936714 1103 21 1.741396032624884 1103 22 1.688824997345911 1103 23 1.822862686550124 1103 24 2.0041005142746755 1103 25 2.0114720895135534 1104 1 1.2226534453517424 1104 2 3.8708391043352606 1104 3 2.233861758612125 1104 4 1.5186094971808686 1104 5 1.1966161625840428 1104 6 0.9266875348793917 1104 7 0.8824335336361102 1104 8 0.8977791354973732 1104 9 0.982920766281687 1104 10 1.1136503752193505 1104 11 1.0076894756649324 1104 12 0.9362285111703983 1104 13 1.11731725542883 1104 14 1.2656895480951107 1104 15 1.2132144216277254 1104 16 1.217714292890271 1104 17 1.0937232378612407 1104 18 1.1765104252221832 1104 19 1.176413252531539 1104 20 1.0951173670438337 1104 21 1.109054623581379 1104 22 1.1096557777339413 1104 23 1.0720461001107395 1104 24 1.1330392780538148 1104 25 1.0603114643189642 1105 1 1.260965942650337 1105 2 3.8823871126633307 1105 3 2.292281126841118 1105 4 1.4494309984790377 1105 5 1.0554323075592364 1105 6 0.9682882012120615 1105 7 0.908465687340609 1105 8 0.8249482030040625 1105 9 1.004618713051734 1105 10 0.9557310011458142 1105 11 0.9893745698695042 1105 12 0.849534858103226 1105 13 0.8785256684224265 1105 14 1.13480487132707 1105 15 0.9856642081386795 1105 16 0.9786688079111627 1105 17 1.0435520198332924 1105 18 1.102536680012335 1105 19 1.0232815134636226 1105 20 1.0778300140715515 1105 21 1.1165291189319717 1105 22 1.1149218500328786 1105 23 1.145635464449711 1105 24 1.2177759594877031 1105 25 1.374943245657903 1106 1 1.1033234174268074 1106 2 3.7337677946508165 1106 3 2.1000067151344624 1106 4 1.1543256569232838 1106 5 0.839316980438845 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2.272162158107527 2314 3 1.0585392902769222 2314 4 0.36935788372397127 2314 5 -0.020495168597030045 2314 6 -0.09499360414831415 2314 7 -0.1502256124159942 2314 8 -0.13478102155950467 2314 9 -0.18973282279162618 2314 10 -0.25599959884383594 2314 11 -0.18457200652746053 2314 12 -0.23531962998195866 2314 13 -0.3712229627803012 2314 14 -1.0118545646724755 2314 15 -0.8415417871702289 2314 16 -0.5883558567445561 2314 17 -0.67438356999007 2314 18 -0.5380048876956209 2314 19 -0.5168657345688814 2314 20 -0.5276903772093888 2314 21 -0.4532895377885282 2314 22 -0.4693232081344263 2314 23 -0.3948968860516189 2314 24 -0.4077558712305134 2314 25 -0.42814123572271257 2315 1 -4.927947932304015 2315 2 -13.865695687313604 2315 3 -7.430460036740733 2315 4 -3.676143200525363 2315 5 -2.5384672484576 2315 6 -1.909722139175031 2315 7 -1.6963220272032018 2315 8 -1.6718324385612213 2315 9 -1.6977395871270176 2315 10 -1.7176338530701045 2315 11 -1.6602727422064518 2315 12 -1.8168338977829137 2315 13 -1.8599663264266013 2315 14 -1.8581413701920795 2315 15 -1.8632181459330894 2315 16 -1.86240059569068 2315 17 -1.8920415914219717 2315 18 -1.710875071126452 2315 19 -1.964629074862117 2315 20 -1.765440085296106 2315 21 -1.874053056393322 2315 22 -1.8096394523177324 2315 23 -1.892987226741738 2315 24 -2.0275007131371794 2315 25 -2.0355681998820856 2316 1 -1.4775083942018945 2316 2 -1.23308661030185 2316 3 -0.8806893428079441 2316 4 -0.9676236517306478 2316 5 -1.0802477327987958 2316 6 -1.2930712051043542 2316 7 -1.3049632599704424 2316 8 -1.623754739028744 2316 9 -1.4721675749975986 2316 10 -1.6517421211758148 2316 11 -1.6723005206803663 2316 12 -1.4781027525334665 2316 13 -1.3573279840017705 2316 14 -1.6802822841306053 2316 15 -2.3200523577857197 2316 16 -1.9613795084429881 2316 17 -1.8647420567169775 2316 18 -1.7736105379668743 2316 19 -1.9021135859125238 2316 20 -2.0528022162414246 2316 21 -1.9761144441475196 2316 22 -2.0097385815951156 2316 23 -2.049607308217098 2316 24 -1.9332374023863892 2316 25 -2.004154521712529 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80132.0 3 0.0 4 763.0 5 3534.0 6 4366.0 7 3867.0 8 3438.0 9 3377.0 10 3274.0 11 3016.0 12 3006.0 13 3010.0 14 3016.0 15 3104.0 16 3241.0 17 3732.0 18 4102.0 19 4940.0 20 6582.0 21 8330.0 22 10323.0 23 12380.0 24 14160.0 25 16035.0 26 18268.0 27 20430.0 28 23460.0 29 27953.0 30 35171.0 31 44550.0 32 56542.0 33 74361.0 34 101492.0 35 144736.0 36 229985.0 37 308714.0 38 775326.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.594714929248624 19.529881961452762 12.651232646665852 25.22417046263276 2 15.317243325040398 21.477750134653366 41.65605683654364 21.5489497037626 3 18.694817900282928 25.352254018488246 30.07685013351329 25.876077947715537 4 11.560243872641701 17.41301274630148 40.155018916806775 30.871724464250043 5 12.077599150241213 38.751579844505 36.90482827629572 12.265992728958071 6 30.010335937356075 38.73896716255249 18.94855592605054 12.302140974040896 7 26.732473108270842 31.932946658677203 23.416456749256806 17.91812348379515 8 23.317703455056343 39.215044630477486 21.360720525753425 16.106531388712746 9 26.093170363734032 15.22497522683685 21.099511517824073 37.582342891605045 10 14.047789419386866 29.686878852929826 36.17807783524247 20.08725389244084 11 33.33149110202277 22.33821815509261 26.454926417403072 17.87536432548155 12 23.16824032004406 24.807874666216776 33.437516361922825 18.586368651816343 13 28.976575002508838 22.802564198280805 26.30000033935922 21.920860459851138 14 19.542486701998723 23.70001493176957 28.696728003273353 28.060770362958355 15 21.67724495277101 33.45700522999773 23.72066731435641 21.14508250287485 16 20.371151433352917 28.989885180509578 30.208569672218573 20.430393713918928 17 20.27763530042575 29.919435925429994 29.233249915402386 20.569678858741867 18 19.938033156285208 28.125587817227384 32.34773958218194 19.58863944430547 19 22.70020114286379 27.263511695519444 28.546821588849248 21.489465572767514 20 22.672428671435803 28.361551901674886 29.545674908906154 19.420344517983157 21 21.63498900715323 27.60879524701787 28.06853137021532 22.687684375613575 22 21.98892874076789 29.86200196059609 28.46102903175448 19.688040266881536 23 20.754472752376483 29.343844414537756 29.223371949162075 20.67831088392369 24 22.31762673687956 27.480233230667494 30.221668034628753 19.98047199782419 25 21.491006932661076 28.6364958549474 29.616909875406023 20.255587336985503 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 202.0 1 202.0 2 354.0 3 506.0 4 506.0 5 506.0 6 1352.5 7 2199.0 8 2199.0 9 2199.0 10 3406.5 11 4614.0 12 4614.0 13 4614.0 14 7699.5 15 10785.0 16 10785.0 17 10785.0 18 19488.5 19 28192.0 20 28192.0 21 28192.0 22 45889.0 23 63586.0 24 63586.0 25 63586.0 26 92162.0 27 120738.0 28 120738.0 29 120738.0 30 157881.0 31 195024.0 32 195024.0 33 195024.0 34 216564.5 35 238105.0 36 238105.0 37 238105.0 38 248559.5 39 259014.0 40 259014.0 41 259014.0 42 264564.5 43 270115.0 44 270115.0 45 270115.0 46 268910.5 47 267706.0 48 267706.0 49 267706.0 50 247840.5 51 227975.0 52 227975.0 53 227975.0 54 193233.0 55 158491.0 56 158491.0 57 158491.0 58 131699.5 59 104908.0 60 104908.0 61 104908.0 62 82511.5 63 60115.0 64 60115.0 65 60115.0 66 45186.0 67 30257.0 68 30257.0 69 30257.0 70 21631.0 71 13005.0 72 13005.0 73 13005.0 74 8888.5 75 4772.0 76 4772.0 77 4772.0 78 3212.0 79 1652.0 80 1652.0 81 1652.0 82 1089.0 83 526.0 84 526.0 85 526.0 86 340.5 87 155.0 88 155.0 89 155.0 90 101.5 91 48.0 92 48.0 93 48.0 94 33.0 95 18.0 96 18.0 97 18.0 98 13.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.491109779533391 3 0.0 4 0.0 5 6.302370272979897E-4 6 4.847977133061459E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.4239885665307295E-4 14 0.0 15 0.0 16 0.0 17 4.847977133061459E-4 18 0.0 19 1.4543931399184376E-4 20 3.8783817064491665E-4 21 5.332774846367604E-4 22 0.0037814221637879376 23 1.9391908532245832E-4 24 0.00179375153923274 25 7.756763412898333E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2062716.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.17596226305409 #Duplication Level Percentage of deduplicated Percentage of total 1 87.85953643996987 66.049251958606 2 9.059950123506585 13.621809371797664 3 1.657471211152255 3.7380597966504134 4 0.5372878969390185 1.615645386587334 5 0.2326153118109916 0.8743539951255831 6 0.13614274731344542 0.6140797220654447 7 0.0892187311772975 0.4694972777699458 8 0.0681011603969575 0.4095656211257496 9 0.047373201722460796 0.32051934224709855 >10 0.2691758812219208 3.6916262342905926 >50 0.02053969843837964 1.0910890914689786 >100 0.018542084190560117 2.8795907452333283 >500 0.001761755295555087 0.9394527679384505 >1k 0.002088006276213332 2.823263550097301 >5k 1.957505883949999E-4 0.8621951389961127 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6339 0.30731327046476586 No Hit CTGTAGGACGTGGAATATGGCAAGA 5888 0.2854488935946587 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5350 0.25936677661878804 No Hit GTATCAACGCAGAGTACTTTTTTTT 4946 0.23978094900121974 No Hit CTTTAGGACGTGAAATATGGCGAGG 4616 0.22378262446211694 No Hit GTCCTACAGTGTGCATTTCTCATTT 3164 0.15338999649006455 No Hit GGTATCAACGCAGAGTACTTTTTTT 3075 0.14907529684163986 No Hit TATCAACGCAGAGTACTTTTTTTTT 2926 0.14185181091337828 No Hit CTGAAGGACCTGGAATATGGCGAGA 2647 0.1283259547121368 No Hit CTGTAGGACCTGGAATATGGCGAGA 2329 0.11290938742900138 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2145 0.10398910950416829 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.847977133061459E-5 0.0 4 0.0 0.0 0.0 4.847977133061459E-5 0.0 5 0.0 0.0 0.0 4.847977133061459E-5 0.0 6 0.0 0.0 0.0 4.847977133061459E-5 0.0 7 0.0 0.0 0.0 9.695954266122918E-5 0.0 8 0.0 0.0 0.0 9.695954266122918E-5 0.0 9 0.0 0.0 0.0 9.695954266122918E-5 0.0 10 0.0 0.0 0.0 9.695954266122918E-5 0.0 11 0.0 0.0 0.0 9.695954266122918E-5 0.0 12 0.0 0.0 0.0 9.695954266122918E-5 0.0 13 0.0 0.0 0.0 9.695954266122918E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 960 0.0 13.773976 4 GGTATCA 1020 0.0 12.736747 1 CGTGCGC 60 4.298403E-4 12.593349 10 TCCAACG 220 0.0 12.450846 18 CCAACGA 160 0.0 12.397179 19 GATATAC 645 0.0 11.930161 1 TGTAGGA 3455 0.0 11.511989 2 GGACCTG 2025 0.0 11.473941 6 AGGACCT 2105 0.0 11.441701 5 CTGTAGG 3370 0.0 11.327885 1 ACTGTTC 710 0.0 11.307408 8 TTAGGAC 1985 0.0 11.13417 3 TATACTG 300 0.0 11.019179 5 GTCCTAA 1560 0.0 10.890295 1 AGGACGT 3565 0.0 10.888919 5 TACGACT 70 0.0015657585 10.794298 4 GTAGGAC 3590 0.0 10.786781 3 GGCGAGG 1455 0.0 10.711357 19 GGACGTG 3530 0.0 10.675806 6 GTCGAGG 195 1.8189894E-12 10.656426 19 >>END_MODULE