FastQCFastQC Report
Thu 26 May 2016
SRR1511807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062716
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71330.34580620890127384No Hit
CTGTAGGACGTGGAATATGGCAAGA64340.31191884874117426No Hit
GTATCAACGCAGAGTACTTTTTTTT60510.2933510963215489No Hit
GTCCTAAAGTGTGTATTTCTCATTT59170.2868548069632465No Hit
CTTTAGGACGTGAAATATGGCGAGG52570.2548581578850409No Hit
GGTATCAACGCAGAGTACTTTTTTT39280.1904285417866541No Hit
GTCCTACAGTGTGCATTTCTCATTT36300.17598156993013095No Hit
TATCAACGCAGAGTACTTTTTTTTT36280.17588461038746972No Hit
CTGAAGGACCTGGAATATGGCGAGA30540.14805722164369695No Hit
CTGTAGGACCTGGAATATGGCGAGA25520.12372037643572843No Hit
GTCCTTCAGTGTGCATTTCTCATTT25440.1233325382650835No Hit
TTTCTAAATTTTCCACCTTTTTCAG21880.10607373967138473No Hit
ATTTAGAAATGTCCACTGTAGGACG21780.10558894195807857No Hit
GAATATGGCAAGAAAACTGAAAATC20850.10108032322433141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC350.002175005216.2806858
CGTGCGC601.47211E-615.828444510
GGCGTGC755.8707883E-815.1953088
GCGTGCG759.710875E-713.9290319
GGTATCA11950.013.8074631
TAGGACC10450.013.0000834
ACTGTTC7550.011.82416258
GGCGAGG14800.011.8083119
GATATAC7700.011.6432131
AATCCCG3300.011.512712519
GTGGCGA600.005889222511.0801817
ACCTTTT7950.010.99058215
CCACCTT7750.010.90657313
TGGCGAA1053.475203E-610.85510618
CACCTTT8050.010.85405414
CCGCTCC700.001495608410.8537919
AAATGTC8000.010.8029157
TGTAGGA37100.010.7547862
TCCAACG2300.010.73711618
TAGAAAT8600.010.7151844
Produced by FastQC (version 0.11.5)