FastQCFastQC Report
Thu 26 May 2016
SRR1511806_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511806_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329005
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69340.2977237060461442No Hit
CTGTAGGACGTGGAATATGGCAAGA65940.2831251972408818No Hit
GTCCTAAAGTGTGTATTTCTCATTT53980.2317727956788414No Hit
GTATCAACGCAGAGTACTTTTTTTT53450.22949714577684463No Hit
CTTTAGGACGTGAAATATGGCGAGG47740.20498023834212464No Hit
GTCCTACAGTGTGCATTTCTCATTT39310.1687845238631948No Hit
GGTATCAACGCAGAGTACTTTTTTT32980.14160553541104462No Hit
TATCAACGCAGAGTACTTTTTTTTT31660.13593787905135454No Hit
CTGTAGGACCTGGAATATGGCGAGA31120.13361929235875405No Hit
CTGAAGGACCTGGAATATGGCGAGA27830.11949308825013255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11000.014.1756961
CTGATCG400.00548813314.167249
TAGGACC12950.013.8572734
TACGGCG552.059726E-413.73822316
CGGTAGG955.6227145E-712.6261251
CGTTAGG904.2330794E-612.2169461
CCGGACA550.003206053712.0206884
TGTAGAA9800.011.0156512
ACTGTTC8000.010.9796118
GGCGAGG15150.010.97267919
ATACGGC1303.5610356E-810.89787615
GAAATGT9850.010.8351816
AGGACCT24000.010.8221975
TTTAGAA10600.010.7500742
TGTAGGA40300.010.6901732
ACCGTGA803.9807212E-410.625438
GTAGGAC42100.010.5889743
GATATAC7300.010.5433911
AAATGTC9450.010.4942517
GGACCTG24050.010.4855246
Produced by FastQC (version 0.11.5)