##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511806_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2329005 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.307661426231373 31.0 30.0 34.0 25.0 34.0 2 26.5506347131071 31.0 26.0 34.0 10.0 34.0 3 28.303592735953767 31.0 28.0 34.0 16.0 34.0 4 32.65626909345407 35.0 32.0 37.0 26.0 37.0 5 33.029503586295434 35.0 35.0 37.0 27.0 37.0 6 33.23433826891741 37.0 35.0 37.0 27.0 37.0 7 33.214926545885476 37.0 35.0 37.0 27.0 37.0 8 33.23167232358883 37.0 35.0 37.0 27.0 37.0 9 34.77017954018991 39.0 35.0 39.0 27.0 39.0 10 34.63294196448698 39.0 35.0 39.0 27.0 39.0 11 34.62959675913105 39.0 35.0 39.0 27.0 39.0 12 34.509375033544366 39.0 35.0 39.0 27.0 39.0 13 34.50659230014534 39.0 35.0 39.0 27.0 39.0 14 35.58509792808517 40.0 36.0 41.0 27.0 41.0 15 35.538827095691076 40.0 36.0 41.0 27.0 41.0 16 35.486731458283685 40.0 36.0 41.0 26.0 41.0 17 35.44366628667607 40.0 36.0 41.0 26.0 41.0 18 35.411019297940534 40.0 36.0 41.0 26.0 41.0 19 35.4335834401386 40.0 36.0 41.0 26.0 41.0 20 35.370263696299496 40.0 36.0 41.0 25.0 41.0 21 35.25412912381038 40.0 36.0 41.0 25.0 41.0 22 35.16093095549387 40.0 36.0 41.0 25.0 41.0 23 35.04825451211998 40.0 36.0 41.0 24.0 41.0 24 34.941307124716346 40.0 35.0 41.0 23.0 41.0 25 34.8301411976359 39.0 35.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9909204757242733 1101 2 3.6219102597152464 1101 3 2.1430396447373283 1101 4 1.2276716317115586 1101 5 0.6816999607315779 1101 6 0.6158971152105011 1101 7 0.47807459549743925 1101 8 0.4965671367772231 1101 9 0.5484271588665379 1101 10 0.5956090566428998 1101 11 0.5857949230553174 1101 12 0.49492240091583994 1101 13 0.40094905617544896 1101 14 0.4343885926641775 1101 15 0.7042127340687969 1101 16 0.5593546445514122 1101 17 0.7078757205557054 1101 18 0.8065915028936388 1101 19 0.7901293327008361 1101 20 0.703153974024417 1101 21 0.6525406540535883 1101 22 0.6846969849333036 1101 23 0.4596541194145729 1101 24 0.5807731443489459 1101 25 0.7537402559482018 1102 1 1.2452871161648709 1102 2 3.8765867712446287 1102 3 2.1621657114322375 1102 4 1.3053935950354116 1102 5 0.8920624856967763 1102 6 0.8186907967453294 1102 7 0.7072956812566815 1102 8 0.5499714822350654 1102 9 0.6786032770048891 1102 10 0.7428438203056729 1102 11 0.705814289998365 1102 12 0.8269795333978394 1102 13 0.757072036263807 1102 14 0.9499329853289495 1102 15 0.9081986930350823 1102 16 0.8836504746064904 1102 17 0.9133698802119383 1102 18 0.9405199662477486 1102 19 1.0015425143264451 1102 20 0.8850325675001827 1102 21 0.8121678403998942 1102 22 1.0162336308208495 1102 23 1.010326515468563 1102 24 0.8026698943338104 1102 25 0.8771524692266652 1103 1 1.3176979214059052 1103 2 3.825927895092736 1103 3 2.38912940123236 1103 4 1.4886611617485244 1103 5 1.071557117032917 1103 6 0.942492536731045 1103 7 1.155153250662913 1103 8 0.9255578199515426 1103 9 0.9567469019360999 1103 10 1.1497620784464857 1103 11 1.2054328045704565 1103 12 1.0636318758214074 1103 13 1.111991418486916 1103 14 1.400287890894326 1103 15 1.154533599303015 1103 16 1.158927221324987 1103 17 1.1754826865536359 1103 18 1.3739274577878575 1103 19 1.2963497225041465 1103 20 1.296074568366663 1103 21 1.3268158035862 1103 22 1.3477819370864381 1103 23 1.5052440242211844 1103 24 1.4362893222227626 1103 25 1.359770878416036 1104 1 1.2650690546905778 1104 2 3.8461388611893206 1104 3 2.3087533440371892 1104 4 1.5423983098780951 1104 5 1.2562129073125163 1104 6 0.9363778037269199 1104 7 0.9092713816828351 1104 8 0.9581657881558243 1104 9 1.0287506158388737 1104 10 1.083193806705161 1104 11 0.9798926289230181 1104 12 0.9782634213296504 1104 13 1.1292036891788513 1104 14 1.2670179431673745 1104 15 1.1747976593964964 1104 16 1.2771385514946871 1104 17 1.1199579661671493 1104 18 1.2546372932946284 1104 19 1.277853603273769 1104 20 1.2681779115930283 1104 21 1.2673215261374935 1104 22 1.2337021229778955 1104 23 1.406366047611094 1104 24 1.3267994618376662 1104 25 1.4182559237556305 1105 1 1.1918438204150412 1105 2 3.9546157005051157 1105 3 2.3080256152987673 1105 4 1.551229855867156 1105 5 1.006617673223964 1105 6 0.9631314493823098 1105 7 0.8195646319297296 1105 8 0.8668639844934205 1105 9 0.9176622396109764 1105 10 0.8905855759125245 1105 11 0.9562283776783431 1105 12 0.7687527610066525 1105 13 0.9429042822405904 1105 14 1.0762195790590852 1105 15 1.098757761588736 1105 16 1.213893930950512 1105 17 1.035294110976885 1105 18 1.251377918448668 1105 19 1.1321410622872392 1105 20 1.2499224377980624 1105 21 1.26536377647448 1105 22 1.2462466790994853 1105 23 1.2581006129626218 1105 24 1.3248686965687497 1105 25 1.4478567417014858 1106 1 0.9384850561123983 1106 2 3.6188139600503817 1106 3 2.0519830275336837 1106 4 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1107 17 0.7409727731598394 1107 18 0.6736672416207696 1107 19 0.7912069584330439 1107 20 0.8961269792457216 1107 21 0.6026545593365427 1107 22 0.8213358179068351 1107 23 0.8134375203987361 1107 24 0.7816026311233344 1107 25 0.9013659218687593 1108 1 0.9636188359737474 1108 2 3.6674134162588388 1108 3 2.1012783770787387 1108 4 1.3723975403629538 1108 5 0.9876662040899191 1108 6 0.7825878226554082 1108 7 0.7811324342208863 1108 8 0.7145426490734934 1108 9 0.7197877080553496 1108 10 0.7101344524949624 1108 11 0.7041416376764573 1108 12 0.6555006081092216 1108 13 0.6427281161280689 1108 14 0.8493517001306969 1108 15 0.771283751798606 1108 16 0.6986329340341513 1108 17 0.6554310275839796 1108 18 0.8534239979769254 1108 19 0.8056067221900349 1108 20 0.7609329661968687 1108 21 0.715638271262975 1108 22 0.6836146203797853 1108 23 0.7648442798523476 1108 24 0.8103003035444374 1108 25 0.7686951027067721 1109 1 0.29407066678815497 1109 2 2.3985230850405834 1109 3 1.1839887009471894 1109 4 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-1.3260273345186135 2315 10 -1.3360815950354379 2315 11 -1.2932807966046695 2315 12 -1.226657794279788 2315 13 -1.3094744465923256 2315 14 -1.3751359436212098 2315 15 -1.3947988325923433 2315 16 -1.420068153884145 2315 17 -1.3840991901309394 2315 18 -1.383304706781587 2315 19 -1.2925331307778265 2315 20 -1.24453264989112 2315 21 -1.397927599684941 2315 22 -1.3098674649727116 2315 23 -1.4237774081793688 2315 24 -1.5147567581935846 2315 25 -1.686445975453438 2316 1 -1.721158655561723 2316 2 -1.5339005172556384 2316 3 -1.1673070668523557 2316 4 -1.3514664837982657 2316 5 -1.6247607581884473 2316 6 -1.7087984023527305 2316 7 -1.7403673650279785 2316 8 -1.8387869734511142 2316 9 -1.851308766646241 2316 10 -2.0735329289240667 2316 11 -2.132437599083687 2316 12 -1.953849425068178 2316 13 -2.0957978193225344 2316 14 -2.166082643705252 2316 15 -2.733258525065679 2316 16 -2.57315383542673 2316 17 -2.5283434612686904 2316 18 -2.363786624454008 2316 19 -2.4005939126835543 2316 20 -2.6860075894786632 2316 21 -2.5534875290684056 2316 22 -2.6817063660808813 2316 23 -2.486445090638469 2316 24 -2.4084339492381446 2316 25 -2.668883642792892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96675.0 3 0.0 4 878.0 5 4180.0 6 5124.0 7 4590.0 8 4120.0 9 4025.0 10 3862.0 11 3687.0 12 3641.0 13 3568.0 14 3647.0 15 3699.0 16 3905.0 17 4216.0 18 4749.0 19 6090.0 20 7709.0 21 9963.0 22 12163.0 23 14662.0 24 16795.0 25 19403.0 26 21483.0 27 24194.0 28 27949.0 29 33305.0 30 41491.0 31 52622.0 32 66783.0 33 87518.0 34 118354.0 35 168935.0 36 261284.0 37 349732.0 38 834004.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.965214759092405 19.12095508597019 12.597783173501131 25.31604698143628 2 15.551425481446476 21.0634082502522 41.33809857042887 22.047067697872457 3 18.97857668832828 24.68934158578449 29.705217464110213 26.626864261777023 4 11.770734712892414 16.812716159905193 39.54989362410128 31.86665550310111 5 12.380892678034103 38.46655548838296 36.585913562605626 12.566638270977315 6 31.037637903599087 38.05126016431213 18.467253074519256 12.44384885756953 7 27.47508056015337 31.578764322103215 22.65375128005307 18.292403837690344 8 23.79960541089435 39.06685472980951 20.784927469026474 16.348612390269665 9 26.049063870622863 15.190521274106326 20.75409885337301 38.00631600189781 10 14.19670631879279 29.529777737703437 35.816024439621216 20.45749150388256 11 33.904650269106334 22.027432315516712 25.92901260409488 18.13890481128207 12 23.367575423839796 24.52424962591321 33.06098527053398 19.04718967971301 13 29.218020945461166 22.464910921034384 26.018537561812984 22.29853057169147 14 19.722284838375188 23.2913196837276 28.332313584556495 28.654081893340717 15 21.942546280493172 33.217747493028135 23.347352195465447 21.492354031013246 16 20.754742905232064 28.679071105472083 29.86674567036138 20.69944031893448 17 20.667449267621187 29.662179162949027 28.867713245163344 20.80265832426644 18 20.252081897634397 27.827634547800457 32.023074231270435 19.897209323294714 19 23.007243032086343 27.106604259258383 28.165042720106552 21.72110998854872 20 22.89512745659168 28.05324542108185 29.304689717297933 19.746937405028532 21 21.809037760671607 27.353687273077014 27.79654132445736 23.040733641794017 22 22.148439177294 29.544205418867275 28.180342651036973 20.12701275280175 23 20.971808477293667 29.01170374555925 28.936392388486382 21.080095388660702 24 22.49730029133238 27.164243190615533 29.98104299997209 20.357413518079998 25 21.702400447921022 28.24908049954873 29.370901106148523 20.677617946381723 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 172.0 1 172.0 2 355.5 3 539.0 4 539.0 5 539.0 6 1467.5 7 2396.0 8 2396.0 9 2396.0 10 3665.0 11 4934.0 12 4934.0 13 4934.0 14 8285.5 15 11637.0 16 11637.0 17 11637.0 18 21065.0 19 30493.0 20 30493.0 21 30493.0 22 48981.5 23 67470.0 24 67470.0 25 67470.0 26 97845.0 27 128220.0 28 128220.0 29 128220.0 30 167686.0 31 207152.0 32 207152.0 33 207152.0 34 231138.5 35 255125.0 36 255125.0 37 255125.0 38 268407.0 39 281689.0 40 281689.0 41 281689.0 42 290790.0 43 299891.0 44 299891.0 45 299891.0 46 300048.0 47 300205.0 48 300205.0 49 300205.0 50 282744.5 51 265284.0 52 265284.0 53 265284.0 54 228683.0 55 192082.0 56 192082.0 57 192082.0 58 161814.5 59 131547.0 60 131547.0 61 131547.0 62 105450.0 63 79353.0 64 79353.0 65 79353.0 66 60462.0 67 41571.0 68 41571.0 69 41571.0 70 30063.5 71 18556.0 72 18556.0 73 18556.0 74 12822.0 75 7088.0 76 7088.0 77 7088.0 78 4806.0 79 2524.0 80 2524.0 81 2524.0 82 1634.0 83 744.0 84 744.0 85 744.0 86 489.0 87 234.0 88 234.0 89 234.0 90 148.5 91 63.0 92 63.0 93 63.0 94 46.0 95 29.0 96 29.0 97 29.0 98 18.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.5107223900335125 3 0.0 4 0.0 5 5.581782778482657E-4 6 9.875461838853932E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.7174716241485097E-4 14 0.0 15 0.0 16 0.0 17 8.157990214705422E-4 18 0.0 19 3.4349432482970194E-4 20 6.869886496594039E-4 21 9.016726026779676E-4 22 0.004508363013389838 23 3.005575342259892E-4 24 0.002146839530185637 25 9.875461838853932E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2329005.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.43011503037944 #Duplication Level Percentage of deduplicated Percentage of total 1 87.10720793955728 64.8339950691643 2 9.365019243512291 13.94078919112674 3 1.9169977703226544 4.280470936742882 4 0.6101348474463542 1.8164962751790061 5 0.2924405128445597 1.0883190505281868 6 0.15886410517806512 0.7094564173561011 7 0.09971892701722554 0.5195463846018676 8 0.07341325980910599 0.43713258978775127 9 0.051507194525561885 0.3450317772086731 >10 0.28342837171976665 3.798062396037452 >50 0.020101428272150623 1.047443746787907 >100 0.016949440898268824 2.5351217461572184 >500 0.0021670483219187607 1.105907771874758 >1k 0.0018156350805228108 2.484183857777496 >5k 2.3427549426100784E-4 1.0580427896697013 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6934 0.2977237060461442 No Hit CTGTAGGACGTGGAATATGGCAAGA 6594 0.2831251972408818 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5398 0.2317727956788414 No Hit GTATCAACGCAGAGTACTTTTTTTT 5345 0.22949714577684463 No Hit CTTTAGGACGTGAAATATGGCGAGG 4774 0.20498023834212464 No Hit GTCCTACAGTGTGCATTTCTCATTT 3931 0.1687845238631948 No Hit GGTATCAACGCAGAGTACTTTTTTT 3298 0.14160553541104462 No Hit TATCAACGCAGAGTACTTTTTTTTT 3166 0.13593787905135454 No Hit CTGTAGGACCTGGAATATGGCGAGA 3112 0.13361929235875405 No Hit CTGAAGGACCTGGAATATGGCGAGA 2783 0.11949308825013255 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.2881037181113823E-4 0.0 10 0.0 0.0 0.0 1.2881037181113823E-4 0.0 11 0.0 0.0 0.0 1.2881037181113823E-4 0.0 12 0.0 0.0 0.0 1.2881037181113823E-4 8.587358120742549E-5 13 0.0 0.0 0.0 1.2881037181113823E-4 1.7174716241485097E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1100 0.0 14.175696 1 CTGATCG 40 0.005488133 14.16724 9 TAGGACC 1295 0.0 13.857273 4 TACGGCG 55 2.059726E-4 13.738223 16 CGGTAGG 95 5.6227145E-7 12.626125 1 CGTTAGG 90 4.2330794E-6 12.216946 1 CCGGACA 55 0.0032060537 12.020688 4 TGTAGAA 980 0.0 11.015651 2 ACTGTTC 800 0.0 10.979611 8 GGCGAGG 1515 0.0 10.972679 19 ATACGGC 130 3.5610356E-8 10.897876 15 GAAATGT 985 0.0 10.835181 6 AGGACCT 2400 0.0 10.822197 5 TTTAGAA 1060 0.0 10.750074 2 TGTAGGA 4030 0.0 10.690173 2 ACCGTGA 80 3.9807212E-4 10.62543 8 GTAGGAC 4210 0.0 10.588974 3 GATATAC 730 0.0 10.543391 1 AAATGTC 945 0.0 10.494251 7 GGACCTG 2405 0.0 10.485524 6 >>END_MODULE