FastQCFastQC Report
Thu 26 May 2016
SRR1511806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329005
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79700.34220622111159055No Hit
CTGTAGGACGTGGAATATGGCAAGA71590.30738448393197954No Hit
GTATCAACGCAGAGTACTTTTTTTT67560.2900809573186833No Hit
GTCCTAAAGTGTGTATTTCTCATTT61550.2642759461658519No Hit
CTTTAGGACGTGAAATATGGCGAGG53360.2291107146614112No Hit
GGTATCAACGCAGAGTACTTTTTTT44960.19304381055429248No Hit
GTCCTACAGTGTGCATTTCTCATTT43970.18879306828452494No Hit
TATCAACGCAGAGTACTTTTTTTTT41390.17771537630876705No Hit
CTGTAGGACCTGGAATATGGCGAGA34440.14787430683918668No Hit
CTGAAGGACCTGGAATATGGCGAGA31430.13495033286746916No Hit
GTCCTTCAGTGTGCATTTCTCATTT24580.10553863130392592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12350.013.9993354
GGTATCA13550.013.0190661
GGCGAGG14600.012.4906819
GGCGTGC1155.349648E-912.3880558
TGGCGAG34600.011.14519418
TGTAGGA39700.011.1266762
TCCAACG2500.011.01782118
AGGACGT37600.011.0154345
CTGTAGG38300.010.9298241
GGACGTG37650.010.9227686
GACGTGA19800.010.7926247
TTAGGAC22450.010.7901333
GTAGGAC38850.010.758823
GCACCGT803.7813466E-410.6846986
GCCTCGA1909.094947E-1210.49724916
AGGACCT23450.010.45152955
GACGTGG19100.010.4422887
GTCCTAA20200.010.4324981
ATAGGAC4200.010.4042673
ATGGCGA38450.010.3002917
Produced by FastQC (version 0.11.5)