FastQCFastQC Report
Thu 26 May 2016
SRR1511805_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511805_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332475
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51920.22259616930513726No Hit
GTCCTACAGTGGACATTTCTAAATT49940.2141073323401108No Hit
CTGTAGGACGTGGAATATGGCAAGA45790.19631507304472715No Hit
GTCCTAAAGTGTGTATTTCTCATTT45320.1943000460883825No Hit
CTTTAGGACGTGAAATATGGCGAGG38910.1668185082369586No Hit
GGTATCAACGCAGAGTACTTTTTTT33600.14405299092166046No Hit
TATCAACGCAGAGTACTTTTTTTTT31820.13642161223592963No Hit
GTCCTACAGTGTGCATTTCTCATTT26610.11408482406027932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.014.382491
GCGACTA604.2970423E-412.59395216
AATCCCG2000.012.27936619
TAGGACC8700.012.15967754
CTACACG803.0417867E-511.8068294
GTATAAG1801.8189894E-1211.6556091
GGCGAGG12850.011.61412619
GGCGTGC1105.357124E-711.1625818
TCCAACG1451.3405952E-911.0742318
GTCCTAT3750.010.9229711
TGGCGAG26650.010.66846518
ACCACCG803.978247E-410.6261465
AGCGGAC803.979035E-410.6259188
AATGTCC7600.010.5637798
ATAGGAC3200.010.3309763
TGTCCAC9150.010.21946310
ATGTCGA1304.159374E-710.17203817
AGGCGTG1304.1605927E-710.171827
AAATGTC8050.010.090597
AAGCGAC750.002777935710.07516114
Produced by FastQC (version 0.11.5)