##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511805_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2332475 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.651775474549567 31.0 30.0 34.0 25.0 34.0 2 26.89132230784896 31.0 26.0 34.0 10.0 34.0 3 28.64167761712344 31.0 28.0 34.0 16.0 34.0 4 33.03664133592 35.0 33.0 37.0 27.0 37.0 5 33.41143935090408 35.0 35.0 37.0 28.0 37.0 6 33.6145364902089 37.0 35.0 37.0 28.0 37.0 7 33.60009260549416 37.0 35.0 37.0 28.0 37.0 8 33.616286562556944 37.0 35.0 37.0 28.0 37.0 9 35.18712140537412 39.0 35.0 39.0 29.0 39.0 10 35.057715088050244 39.0 35.0 39.0 28.0 39.0 11 35.065370475567796 39.0 35.0 39.0 28.0 39.0 12 34.95006634583436 39.0 35.0 39.0 27.0 39.0 13 34.945773052229924 39.0 35.0 39.0 27.0 39.0 14 36.05174632096807 40.0 36.0 41.0 27.0 41.0 15 36.00414966934265 40.0 36.0 41.0 27.0 41.0 16 35.95773330903868 40.0 36.0 41.0 27.0 41.0 17 35.91359350046624 40.0 36.0 41.0 27.0 41.0 18 35.88572439147258 40.0 36.0 41.0 27.0 41.0 19 35.91052036999325 40.0 36.0 41.0 27.0 41.0 20 35.84266455160291 40.0 36.0 41.0 27.0 41.0 21 35.73514271321236 40.0 36.0 41.0 27.0 41.0 22 35.640799151116305 40.0 36.0 41.0 27.0 41.0 23 35.54185618281011 40.0 36.0 41.0 27.0 41.0 24 35.43155232103238 40.0 36.0 41.0 26.0 41.0 25 35.33294547637166 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8353749552183416 1101 2 3.5029095504082264 1101 3 2.08154835727575 1101 4 1.0513663789699308 1101 5 0.5684054466951736 1101 6 0.5582909930053717 1101 7 0.3273614044977933 1101 8 0.2918792501675327 1101 9 0.5125798794716161 1101 10 0.5050408546960696 1101 11 0.43300545818455305 1101 12 0.3202757582144855 1101 13 0.28584061337966915 1101 14 0.2471973173454387 1101 15 0.5801636003711792 1101 16 0.4370486680464154 1101 17 0.32824322454571586 1101 18 0.4448586480126622 1101 19 0.5850893331386473 1101 20 0.36544271346262036 1101 21 0.5316100053731034 1101 22 0.47660332285370544 1101 23 0.372163459766206 1101 24 0.40582418962674893 1101 25 0.3142205211701139 1102 1 1.4789541658246286 1102 2 4.139080623785151 1102 3 2.4287695443728623 1102 4 1.5180416445458604 1102 5 1.3044716949072281 1102 6 1.1750810632495359 1102 7 1.0078753916082022 1102 8 0.9147158611235753 1102 9 1.0407983385301591 1102 10 1.1413349124728924 1102 11 1.1422348028622977 1102 12 1.0984986590172952 1102 13 1.0577153963398942 1102 14 1.2741950466334728 1102 15 1.0186371355358332 1102 16 1.1616912114653317 1102 17 1.2661944798057192 1102 18 1.162213731202499 1102 19 1.2722283260463882 1102 20 1.228216120128188 1102 21 1.1867273677938286 1102 22 1.3399844985799518 1102 23 1.402499534828685 1102 24 0.9851626258512596 1102 25 1.0926591638376735 1103 1 1.2117754013467952 1103 2 3.7921805856380573 1103 3 2.3231381155611146 1103 4 1.4962191798396063 1103 5 1.0011019472424465 1103 6 0.9096934771522953 1103 7 1.1002001395398153 1103 8 0.8288482278003642 1103 9 0.8654901585593322 1103 10 0.9872123142627984 1103 11 1.0530160671715691 1103 12 0.9437484016836493 1103 13 1.0403566026225448 1103 14 1.1816144474496042 1103 15 0.9790432208864956 1103 16 0.9712590380715298 1103 17 0.9744917688585559 1103 18 1.1257932505403474 1103 19 1.1850065478121081 1103 20 1.241793366452768 1103 21 1.2767468190774451 1103 22 1.2372715309623814 1103 23 1.3263950974455838 1103 24 1.2890614255904467 1103 25 1.285665003175822 1104 1 1.2408851648400763 1104 2 3.736987632126251 1104 3 2.245505707697987 1104 4 1.4311558324888338 1104 5 1.0666377240591203 1104 6 0.9615279592097465 1104 7 0.8223773734810464 1104 8 0.9075569934150636 1104 9 1.0324512271363915 1104 10 1.031698827126263 1104 11 0.9777315224211165 1104 12 0.9983189414526947 1104 13 1.0743963114849961 1104 14 1.201236970214552 1104 15 1.1644105531766584 1104 16 1.1474758590392469 1104 17 1.0353532457751555 1104 18 1.1306377006704693 1104 19 1.1202889625337562 1104 20 1.0002602834713414 1104 21 1.176882288069244 1104 22 1.1599153546455696 1104 23 0.9940255286251727 1104 24 1.0313686921185976 1104 25 0.9986707195933704 1105 1 1.1631987459619992 1105 2 3.697171062020942 1105 3 2.208754118184082 1105 4 1.3536557558746054 1105 5 1.0222487755128284 1105 6 0.8001913463074146 1105 7 0.6548517704447789 1105 8 0.7029895923281515 1105 9 0.8609165916422157 1105 10 0.7283140823873993 1105 11 0.7705191307017074 1105 12 0.6965047370302884 1105 13 0.76453072266915 1105 14 0.8672986758303836 1105 15 0.9538127379882866 1105 16 0.91088576615401 1105 17 0.9891152718955709 1105 18 0.9011524722027175 1105 19 1.0478639359861575 1105 20 1.069147686466927 1105 21 1.1746274644064059 1105 22 0.8814716786515717 1105 23 0.9016758657997812 1105 24 1.0396426177145557 1105 25 1.0643464951607697 1106 1 0.9207314927170067 1106 2 3.6131120935364684 1106 3 2.084034586268409 1106 4 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0.6772569720325947 1107 17 0.6429034226750758 1107 18 0.4516830284128943 1107 19 0.5763165844968157 1107 20 0.5897042683215759 1107 21 0.5376288593999305 1107 22 0.7322821034603137 1107 23 0.7606545945209859 1107 24 0.7009850063799945 1107 25 0.8455944687033465 1108 1 0.8325639553607083 1108 2 3.615246620579274 1108 3 1.9365701613735276 1108 4 1.1781146768697681 1108 5 0.6585158343699149 1108 6 0.5880175784325061 1108 7 0.565698775018312 1108 8 0.4483167438888529 1108 9 0.5016721810545306 1108 10 0.5106614163495777 1108 11 0.4944100788413266 1108 12 0.411969041007481 1108 13 0.4691620553118625 1108 14 0.4645472037282872 1108 15 0.5869438561119722 1108 16 0.4808908567928398 1108 17 0.509141737856929 1108 18 0.45488666351003815 1108 19 0.5646776900154435 1108 20 0.5118094607086761 1108 21 0.5516871964194081 1108 22 0.568548061016557 1108 23 0.6154083187467876 1108 24 0.7056302772708563 1108 25 0.5962948964915 1109 1 0.6482991717612698 1109 2 2.7599086862911015 1109 3 1.5864228251585857 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0.029784147894439172 2313 24 -0.05604128440239009 2313 25 -0.1837172196083472 2314 1 0.0384145074077864 2314 2 2.1882674513782767 2314 3 0.9003633745107109 2314 4 0.22476731297899022 2314 5 -0.1013405516367456 2314 6 -0.30970618601431 2314 7 -0.4916319746453439 2314 8 -0.48454270175049174 2314 9 -0.46949893729433256 2314 10 -0.5533737173671156 2314 11 -0.4477864826495548 2314 12 -0.5513345583639406 2314 13 -0.5312832816534794 2314 14 -1.1917242877092846 2314 15 -1.0114188694107042 2314 16 -0.8921643074159107 2314 17 -0.8482406479251736 2314 18 -0.6682981954562663 2314 19 -0.6569805864745888 2314 20 -0.6746972674760414 2314 21 -0.927654804991711 2314 22 -0.7909742693757167 2314 23 -0.4799404864822847 2314 24 -0.5778068294986412 2314 25 -0.44017740005849504 2315 1 -4.403262739055521 2315 2 -13.687165827607776 2315 3 -7.0850357416281895 2315 4 -3.255876118738378 2315 5 -2.0604731883938854 2315 6 -1.3459171221007864 2315 7 -1.1518725722173997 2315 8 -1.1008444397592214 2315 9 -1.1868824138520324 2315 10 -1.1743165925812349 2315 11 -1.1546667822102705 2315 12 -1.024307403920652 2315 13 -1.0093070351019975 2315 14 -1.19732563383657 2315 15 -1.290521252451498 2315 16 -1.344982831497326 2315 17 -1.2935349871266197 2315 18 -1.3544836737743466 2315 19 -1.2988822894183585 2315 20 -1.3139434881824101 2315 21 -1.3910175341052522 2315 22 -1.2567891243785638 2315 23 -1.2887788936159694 2315 24 -1.3878713632851003 2315 25 -1.312732090939008 2316 1 -1.7513368794390978 2316 2 -1.5553007501891614 2316 3 -1.191358091655168 2316 4 -1.3034212250970079 2316 5 -1.5163212778626658 2316 6 -1.5695451285101 2316 7 -1.60255192670801 2316 8 -1.6737140993462134 2316 9 -1.7926212629552225 2316 10 -1.928721973461208 2316 11 -2.102270749607797 2316 12 -1.9025079729106977 2316 13 -1.7998174297979617 2316 14 -2.1329657885103046 2316 15 -2.6114145709060637 2316 16 -2.299093702804207 2316 17 -2.1671860883185587 2316 18 -2.088131341405685 2316 19 -2.2114552525811604 2316 20 -2.3590171462324747 2316 21 -2.3585724301991675 2316 22 -2.3536094020064624 2316 23 -2.2586218082184075 2316 24 -2.1691739907764784 2316 25 -2.280739685648051 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80647.0 3 2.0 4 763.0 5 3647.0 6 4528.0 7 4067.0 8 3625.0 9 3592.0 10 3408.0 11 3281.0 12 3286.0 13 3202.0 14 3263.0 15 3344.0 16 3542.0 17 3807.0 18 4381.0 19 5457.0 20 6848.0 21 8703.0 22 10964.0 23 12946.0 24 15130.0 25 17365.0 26 19697.0 27 22259.0 28 26214.0 29 31821.0 30 39611.0 31 50458.0 32 65460.0 33 86376.0 34 118386.0 35 170436.0 36 266149.0 37 358727.0 38 867083.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02271192617284 19.12432073226937 12.357731594122123 25.495235747435665 2 15.912127079715042 21.39069394122155 40.38005106043361 22.317127918629804 3 18.578634283325652 25.181448890127438 29.93228223239263 26.307634594154276 4 11.88368578441355 17.17643275919356 39.30803116860845 31.631850287784435 5 12.572927170580126 38.177052250324124 36.51846287874111 12.731557700354646 6 30.70188676656709 38.033772126891144 18.68403948456048 12.580301621981294 7 27.101126485814426 31.702633468740288 23.229830973536693 17.966409071908597 8 24.14645387410369 37.93086742623179 21.19795496200388 16.72472373766064 9 26.339532042144075 14.960246090526159 20.703244407764284 37.996977459565485 10 14.419404280860459 29.238512738614563 35.62220388214236 20.719879098382616 11 33.81052315673265 22.245468868905345 25.65412276658914 18.289885207772862 12 22.966977138018628 24.716877994405085 32.88841252317817 19.42773234439812 13 29.029317809895332 22.289990066337317 26.003044836960598 22.67764728680675 14 20.269756374666397 22.670939667091822 28.36321932710962 28.69608463113217 15 22.329671271932174 32.042487057739095 23.644154814092328 21.9836868562364 16 21.412190913085887 28.321332490166025 28.89261406874672 21.37386252800137 17 21.09792142380026 28.94958202963221 28.582802206423942 21.369694340143592 18 20.843953311396692 27.620274601013943 30.96491923814832 20.570852849441042 19 23.085718646515193 27.038870415371186 28.050288364084736 21.82512257402888 20 23.098264022420953 27.690066414829655 28.858446079055554 20.35322348369383 21 22.333359200191044 27.129288985820533 27.686812661498156 22.85053915249027 22 22.561088809170705 28.80418867741705 27.89147408465934 20.74324842875291 23 21.687104951877174 28.322820153236762 28.447066177514042 21.543008717372018 24 22.5728960783389 27.10700857046085 29.270203178659166 21.049892172541085 25 22.206201947213692 27.794848445483115 28.78599041956114 21.212959187742044 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 292.0 1 292.0 2 474.5 3 657.0 4 657.0 5 657.0 6 1607.5 7 2558.0 8 2558.0 9 2558.0 10 3670.0 11 4782.0 12 4782.0 13 4782.0 14 8136.5 15 11491.0 16 11491.0 17 11491.0 18 20869.5 19 30248.0 20 30248.0 21 30248.0 22 48127.5 23 66007.0 24 66007.0 25 66007.0 26 95173.5 27 124340.0 28 124340.0 29 124340.0 30 161031.0 31 197722.0 32 197722.0 33 197722.0 34 221064.5 35 244407.0 36 244407.0 37 244407.0 38 259085.5 39 273764.0 40 273764.0 41 273764.0 42 283178.5 43 292593.0 44 292593.0 45 292593.0 46 296040.5 47 299488.0 48 299488.0 49 299488.0 50 285187.5 51 270887.0 52 270887.0 53 270887.0 54 237241.5 55 203596.0 56 203596.0 57 203596.0 58 172843.0 59 142090.0 60 142090.0 61 142090.0 62 114732.0 63 87374.0 64 87374.0 65 87374.0 66 66945.5 67 46517.0 68 46517.0 69 46517.0 70 33878.5 71 21240.0 72 21240.0 73 21240.0 74 14752.5 75 8265.0 76 8265.0 77 8265.0 78 5585.5 79 2906.0 80 2906.0 81 2906.0 82 1888.5 83 871.0 84 871.0 85 871.0 86 568.5 87 266.0 88 266.0 89 266.0 90 175.5 91 85.0 92 85.0 93 85.0 94 53.0 95 21.0 96 21.0 97 21.0 98 14.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.455363937448419 3 0.0 4 0.0 5 4.716020536125789E-4 6 8.145853653308182E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 8.57458279295598E-5 14 0.0 15 0.0 16 0.0 17 5.573478815421387E-4 18 0.0 19 3.001103977534593E-4 20 6.002207955069186E-4 21 7.717124513660383E-4 22 0.003944308084759751 23 2.572374837886794E-4 24 0.0019292811284150953 25 6.002207955069186E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2332475.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.71886242352682 #Duplication Level Percentage of deduplicated Percentage of total 1 85.1186058401245 62.74846793610544 2 10.559323992883021 15.568427054335787 3 2.4121555321887986 5.3346408546472555 4 0.8034597539663941 2.3692055626195723 5 0.3545999326330923 1.3070351824585404 6 0.19373036531713658 0.856894928884415 7 0.10879932503067705 0.5614393731596342 8 0.07642265047878026 0.4507032685349183 9 0.055224271831348266 0.3663963447817149 >10 0.28056533204847683 3.6682806002017196 >50 0.01846316169585296 0.9713122506437801 >100 0.015476692663227366 2.3661828450709574 >500 0.0016453365904250444 0.9322616024285633 >1k 0.0014690505271644045 2.2738415395687492 >5k 5.8762021086576174E-5 0.22491065655904718 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5192 0.22259616930513726 No Hit GTCCTACAGTGGACATTTCTAAATT 4994 0.2141073323401108 No Hit CTGTAGGACGTGGAATATGGCAAGA 4579 0.19631507304472715 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4532 0.1943000460883825 No Hit CTTTAGGACGTGAAATATGGCGAGG 3891 0.1668185082369586 No Hit GGTATCAACGCAGAGTACTTTTTTT 3360 0.14405299092166046 No Hit TATCAACGCAGAGTACTTTTTTTTT 3182 0.13642161223592963 No Hit GTCCTACAGTGTGCATTTCTCATTT 2661 0.11408482406027932 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.28729139647799E-5 0.0 2 0.0 0.0 0.0 4.28729139647799E-5 0.0 3 0.0 0.0 0.0 4.28729139647799E-5 0.0 4 0.0 0.0 0.0 4.28729139647799E-5 0.0 5 0.0 0.0 0.0 4.28729139647799E-5 0.0 6 0.0 0.0 0.0 4.28729139647799E-5 0.0 7 0.0 0.0 0.0 4.28729139647799E-5 0.0 8 0.0 0.0 0.0 4.28729139647799E-5 0.0 9 0.0 0.0 0.0 1.286187418943397E-4 0.0 10 0.0 0.0 0.0 1.286187418943397E-4 0.0 11 0.0 0.0 0.0 1.714916558591196E-4 0.0 12 0.0 0.0 0.0 1.714916558591196E-4 0.0 13 0.0 0.0 0.0 1.714916558591196E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1035 0.0 14.38249 1 GCGACTA 60 4.2970423E-4 12.593952 16 AATCCCG 200 0.0 12.279366 19 TAGGACC 870 0.0 12.1596775 4 CTACACG 80 3.0417867E-5 11.806829 4 GTATAAG 180 1.8189894E-12 11.655609 1 GGCGAGG 1285 0.0 11.614126 19 GGCGTGC 110 5.357124E-7 11.162581 8 TCCAACG 145 1.3405952E-9 11.07423 18 GTCCTAT 375 0.0 10.922971 1 TGGCGAG 2665 0.0 10.668465 18 ACCACCG 80 3.978247E-4 10.626146 5 AGCGGAC 80 3.979035E-4 10.625918 8 AATGTCC 760 0.0 10.563779 8 ATAGGAC 320 0.0 10.330976 3 TGTCCAC 915 0.0 10.219463 10 ATGTCGA 130 4.159374E-7 10.172038 17 AGGCGTG 130 4.1605927E-7 10.17182 7 AAATGTC 805 0.0 10.09059 7 AAGCGAC 75 0.0027779357 10.075161 14 >>END_MODULE