FastQCFastQC Report
Thu 26 May 2016
SRR1511805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332475
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63670.2729718432137536No Hit
GTCCTACAGTGGACATTTCTAAATT54810.23498644144095862No Hit
CTGTAGGACGTGGAATATGGCAAGA49950.2141502052540756No Hit
GTCCTAAAGTGTGTATTTCTCATTT48730.20891970975037244No Hit
CTTTAGGACGTGAAATATGGCGAGG43290.1855968445535322No Hit
GGTATCAACGCAGAGTACTTTTTTT42040.1802377303079347No Hit
TATCAACGCAGAGTACTTTTTTTTT40480.17354955572942904No Hit
GTCCTACAGTGTGCATTTCTCATTT28540.12235929645548183No Hit
CTGAAGGACCTGGAATATGGCGAGA24740.10606758914886547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1900.013.99579918
GGTATCA13150.013.0564371
TAGGACC9000.012.1394094
ATTGCGA802.8766251E-511.87143617
TTGCGAG752.0804565E-411.39657918
CCAACGT855.3415817E-511.17311719
TGGACCG951.3602461E-511.0004725
GGCGAGG12250.010.69882919
GTCCTAC26000.010.4922971
GCGTGCG1002.4077473E-510.4468649
GGACCTG17200.010.159746
GTCCTAA14400.010.1346051
TCCTACA28300.010.0711042
AGGACCT18650.010.0352835
AAATGTC8150.010.0215317
TATGTCG951.6496127E-49.99699916
TAGGACT3900.09.9876094
ATTTAGA9400.09.94434451
GAAATGT8900.09.9239866
TAAGCTG2400.09.8962575
Produced by FastQC (version 0.11.5)