##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511805_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2332475 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21815153431441 31.0 31.0 34.0 28.0 34.0 2 31.408213164128234 33.0 31.0 34.0 28.0 34.0 3 31.528078971907522 34.0 31.0 34.0 28.0 34.0 4 35.086070375888276 37.0 35.0 37.0 32.0 37.0 5 34.76885668656684 37.0 35.0 37.0 32.0 37.0 6 34.71652729396885 37.0 35.0 37.0 32.0 37.0 7 34.574431880298825 37.0 35.0 37.0 31.0 37.0 8 34.50437625269296 37.0 35.0 37.0 31.0 37.0 9 36.066093312897245 39.0 37.0 39.0 31.0 39.0 10 35.888750361740215 39.0 37.0 39.0 30.0 39.0 11 35.870897651636135 39.0 37.0 39.0 30.0 39.0 12 35.72200345126957 39.0 35.0 39.0 30.0 39.0 13 35.71402437325159 39.0 35.0 39.0 30.0 39.0 14 36.80807125478301 40.0 37.0 41.0 30.0 41.0 15 36.75962100344055 40.0 37.0 41.0 30.0 41.0 16 36.67621860898831 40.0 36.0 41.0 30.0 41.0 17 36.619433005712814 40.0 36.0 41.0 30.0 41.0 18 36.5579519608999 40.0 36.0 41.0 30.0 41.0 19 36.578187118833 40.0 37.0 41.0 30.0 41.0 20 36.45560531195404 40.0 36.0 41.0 30.0 41.0 21 36.35036988606523 40.0 36.0 41.0 30.0 41.0 22 36.440443305930394 40.0 37.0 41.0 30.0 41.0 23 36.34752569695281 40.0 37.0 41.0 30.0 41.0 24 36.24707703190817 40.0 36.0 41.0 30.0 41.0 25 35.831767971789624 39.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2144114212786974 1101 2 0.16093889468655576 1101 3 0.22812380622602646 1101 4 0.22440940847078394 1101 5 0.24328338640143699 1101 6 0.2766475834955173 1101 7 0.2821510015326467 1101 8 0.3410660362201057 1101 9 0.26320838661445123 1101 10 0.21247021585105585 1101 11 0.06316785190064422 1101 12 0.31188848009134773 1101 13 0.42945574427265854 1101 14 0.5041545261851823 1101 15 0.16778129663101993 1101 16 0.1817678983014659 1101 17 0.18574869861168963 1101 18 0.1575943988620594 1101 19 0.19209278315773304 1101 20 0.23818281458910917 1101 21 0.16704449935551224 1101 22 0.09566477452575839 1101 23 -0.0055117603725349795 1101 24 -0.012939633305421694 1101 25 0.0458017714830774 1102 1 0.539738616313727 1102 2 0.5116880958071235 1102 3 0.6265164255007072 1102 4 0.5057114187004075 1102 5 0.5573015544045887 1102 6 0.5668143923364539 1102 7 0.727231775517545 1102 8 0.7277548323874186 1102 9 0.7461337447747951 1102 10 0.9717585953481489 1102 11 0.907237246482822 1102 12 0.8790304633890003 1102 13 1.003100902667562 1102 14 1.265187299797205 1102 15 1.1554514760059647 1102 16 1.1765760184099179 1102 17 1.2468735605426602 1102 18 1.1446870361113781 1102 19 1.296994322954724 1102 20 1.2412959581603573 1102 21 1.1469811981542506 1102 22 0.9444830282718755 1102 23 1.0205357817507732 1102 24 1.1733905006645102 1102 25 1.3271289323685878 1103 1 0.41244855544308834 1103 2 0.5064289799807362 1103 3 0.5111694571623495 1103 4 0.5057401882403596 1103 5 0.4905841127941244 1103 6 0.5708491556037742 1103 7 0.5756046603798737 1103 8 0.5141289194212106 1103 9 0.7757606609414509 1103 10 0.6732091731217693 1103 11 0.6610797918887314 1103 12 0.7414971286062126 1103 13 0.732385680923592 1103 14 0.9203470289418689 1103 15 0.9081379690583375 1103 16 0.7488722252009836 1103 17 0.6185784324995396 1103 18 0.8001876074393053 1103 19 0.9107513898847515 1103 20 0.7739003020973527 1103 21 0.6507720791763916 1103 22 0.770204402524719 1103 23 0.8952490471660326 1103 24 0.8407177686760505 1103 25 0.8567276280323171 1104 1 0.4381034290183159 1104 2 0.3800415991620447 1104 3 0.4364972431780636 1104 4 0.454332972316152 1104 5 0.5408586743474402 1104 6 0.4938990291569425 1104 7 0.5344289348939526 1104 8 0.5076848470666135 1104 9 0.5741011290310354 1104 10 0.7100101471373108 1104 11 0.7172457526420075 1104 12 0.6982489313058906 1104 13 0.723993493465926 1104 14 0.7580581596447686 1104 15 0.7286897641537706 1104 16 0.8721742151814453 1104 17 0.9069440008989886 1104 18 0.8137815605101935 1104 19 0.7668225706580287 1104 20 0.8600135435873781 1104 21 0.9444732226909736 1104 22 1.029368830455489 1104 23 1.026767192637756 1104 24 0.9970818389132248 1104 25 1.0723768698449945 1105 1 0.3158941666560189 1105 2 0.3715665226455087 1105 3 0.4192505056588267 1105 4 0.3605112878766974 1105 5 0.3894309038791519 1105 6 0.38497416025983 1105 7 0.4861426358425902 1105 8 0.5205000940595781 1105 9 0.6144090585936581 1105 10 0.6749169215013069 1105 11 0.7969679504687974 1105 12 0.8112507056709077 1105 13 0.8034343268148589 1105 14 0.8114238279543002 1105 15 0.875419488274666 1105 16 0.7797924669331309 1105 17 0.6928721159770603 1105 18 0.8436563800553998 1105 19 0.8543016251499722 1105 20 0.9320154950392521 1105 21 0.9460524013905243 1105 22 0.8188189377256947 1105 23 0.9635739238304026 1105 24 0.9758643246326457 1105 25 1.0251265597373234 1106 1 0.26304543218246934 1106 2 0.3043271587852878 1106 3 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0.5144196401106953 1107 16 0.6434081256563502 1107 17 0.7290729491772723 1107 18 0.70952783187672 1107 19 0.5933953301230801 1107 20 0.525060732686363 1107 21 0.6387649441537206 1107 22 0.6351926346641434 1107 23 0.6078990126782102 1107 24 0.5815825327776949 1107 25 0.6233597261037431 1108 1 -0.11216767838370956 1108 2 -0.015363573253395657 1108 3 -0.01614844516031866 1108 4 -0.001063061583124636 1108 5 0.007079894089002892 1108 6 -0.011617236809421172 1108 7 -0.01117908315070082 1108 8 0.10595074578196062 1108 9 0.11111794814326004 1108 10 0.030855958660289673 1108 11 0.1580943083952704 1108 12 0.1349173134349826 1108 13 0.14603719208447075 1108 14 0.24133641408498363 1108 15 0.3005713028413979 1108 16 0.284319574537065 1108 17 0.30829991055877315 1108 18 0.23032170147113362 1108 19 0.2196396778379892 1108 20 0.24363553389198955 1108 21 0.29975109798404986 1108 22 0.3374658195036062 1108 23 0.2835388579632081 1108 24 0.31864638651558863 1108 25 0.387345136338034 1109 1 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-0.4759945947159139 2315 1 -0.6879333761554456 2315 2 -0.596691232684897 2315 3 -0.7348478433531547 2315 4 -0.6948536967653496 2315 5 -0.7622882053918616 2315 6 -0.8610352579071687 2315 7 -0.7858321186141879 2315 8 -0.7914219648940417 2315 9 -0.9597727276554053 2315 10 -0.9414143260087187 2315 11 -1.001632367602582 2315 12 -0.9627983571291381 2315 13 -0.9761251320634727 2315 14 -1.011817658987404 2315 15 -1.221041499554552 2315 16 -1.1817216070471943 2315 17 -1.202211466542316 2315 18 -1.1828051759890243 2315 19 -1.1412851829592299 2315 20 -1.1332546751476613 2315 21 -1.232158450357609 2315 22 -1.19768884391695 2315 23 -1.1137078637746285 2315 24 -1.0527918012115265 2315 25 -1.1228647672324286 2316 1 -1.7738234858658792 2316 2 -1.3179709288965356 2316 3 -1.1377787347708903 2316 4 -1.1215783456429023 2316 5 -1.2898226023114674 2316 6 -1.2362754027707297 2316 7 -1.1757711729134925 2316 8 -1.2949245032763415 2316 9 -1.6089680890785303 2316 10 -1.6642636499242869 2316 11 -1.6125276889675035 2316 12 -1.6351794496554248 2316 13 -1.664539726331057 2316 14 -1.8098077997633837 2316 15 -1.8564126919810988 2316 16 -1.7575578221188906 2316 17 -1.9203360680040333 2316 18 -1.9483241545864942 2316 19 -1.7603270356104304 2316 20 -1.7785382300274009 2316 21 -2.091566917354548 2316 22 -2.092892691139852 2316 23 -1.9213359887235342 2316 24 -1.8597572285263198 2316 25 -1.8818855280113596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28064.0 3 2.0 4 510.0 5 4795.0 6 6059.0 7 4845.0 8 3955.0 9 3515.0 10 3406.0 11 3224.0 12 3027.0 13 2986.0 14 2927.0 15 2861.0 16 3027.0 17 3234.0 18 3405.0 19 3812.0 20 4803.0 21 6202.0 22 7938.0 23 9898.0 24 12296.0 25 14742.0 26 17131.0 27 19661.0 28 23013.0 29 27990.0 30 36369.0 31 46886.0 32 61170.0 33 80571.0 34 106124.0 35 145269.0 36 213506.0 37 390401.0 38 1024851.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13145918246109 19.778843653733237 12.888188062533684 25.20150910127199 2 14.715827608012946 21.078082294558357 41.28065681304194 22.925433284386756 3 18.536747446382062 25.476114427807374 30.320582214171644 25.666555911638923 4 12.292607637809622 17.594014941210517 39.04037556672633 31.07300185425353 5 12.545918949355569 38.349559529741924 36.513359058523235 12.591162462379268 6 30.774949356370378 38.22926290742666 18.45863299713823 12.537154739064727 7 27.058339317677575 31.7289145650007 23.253625440787147 17.95912067653458 8 24.180452094877758 38.05378407056882 21.092058864510875 16.673704970042554 9 26.25918820137408 14.89902356938445 20.585815496414753 38.25597273282672 10 14.39852517175961 29.372190484356747 35.57860212864018 20.650682215243464 11 33.92542256615826 22.24559748764724 25.554357495793095 18.2746224504014 12 22.91818776192671 24.79272017920878 32.907576715720424 19.381515343144084 13 28.954822666909614 22.239638152606137 26.168469115424603 22.637070065059646 14 20.25329317570392 22.639085092015993 28.39760340410937 28.710018328170722 15 22.371901092187482 32.034083966602 23.633093602289414 21.960921338921104 16 21.3844276010912 28.442429987399642 28.823484773457185 21.349657638051973 17 21.08987234589867 28.980589288202445 28.57080997652708 21.3587283893718 18 20.850368193058692 27.623697090468823 30.921228636399494 20.604706080072987 19 23.085263507647454 27.005176904361246 28.122830898509093 21.786728689482203 20 23.10369886065231 27.731872796064266 28.811970117578966 20.352458225704456 21 22.361311482438182 27.077246272736044 27.794381504624916 22.767060740200858 22 22.66022143860063 28.594347206293747 28.049732580199144 20.695698774906486 23 21.611850073419866 28.284933386209925 28.55846257730522 21.544753963064984 24 22.63754958996766 27.062949085846753 29.337109738262722 20.96239158592286 25 22.193430666322538 27.772356733665625 28.78017075431495 21.254041845696886 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 280.0 1 280.0 2 455.0 3 630.0 4 630.0 5 630.0 6 1616.5 7 2603.0 8 2603.0 9 2603.0 10 3811.5 11 5020.0 12 5020.0 13 5020.0 14 8275.0 15 11530.0 16 11530.0 17 11530.0 18 20809.5 19 30089.0 20 30089.0 21 30089.0 22 48500.5 23 66912.0 24 66912.0 25 66912.0 26 95743.5 27 124575.0 28 124575.0 29 124575.0 30 161558.5 31 198542.0 32 198542.0 33 198542.0 34 221760.5 35 244979.0 36 244979.0 37 244979.0 38 259208.0 39 273437.0 40 273437.0 41 273437.0 42 283954.5 43 294472.0 44 294472.0 45 294472.0 46 296649.5 47 298827.0 48 298827.0 49 298827.0 50 284383.0 51 269939.0 52 269939.0 53 269939.0 54 236375.0 55 202811.0 56 202811.0 57 202811.0 58 172194.5 59 141578.0 60 141578.0 61 141578.0 62 114415.5 63 87253.0 64 87253.0 65 87253.0 66 66731.5 67 46210.0 68 46210.0 69 46210.0 70 33595.5 71 20981.0 72 20981.0 73 20981.0 74 14462.0 75 7943.0 76 7943.0 77 7943.0 78 5344.5 79 2746.0 80 2746.0 81 2746.0 82 1794.0 83 842.0 84 842.0 85 842.0 86 530.5 87 219.0 88 219.0 89 219.0 90 132.5 91 46.0 92 46.0 93 46.0 94 28.0 95 10.0 96 10.0 97 10.0 98 5.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40742130140730337 2 0.0 3 0.0 4 0.0 5 0.027824521163142157 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 8.57458279295598E-5 17 0.0 18 1.286187418943397E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004201545568548431 25 4.28729139647799E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2332475.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.37273195627608 #Duplication Level Percentage of deduplicated Percentage of total 1 83.70983127656669 59.74599349807491 2 11.464648503408341 16.365265692133708 3 2.6983421313699223 5.777641490057765 4 0.8777728581233303 2.505961876853232 5 0.38985526524064384 1.3912517673881692 6 0.2185144649086439 0.9357584599496243 7 0.13470295896230472 0.6729882728613683 8 0.09118447649889623 0.5206468159783261 9 0.06294265224500832 0.4043150142570069 >10 0.3094342211590436 3.924393432727861 >50 0.021342345213531812 1.0795253050309999 >100 0.01765192809609566 2.593315395777027 >500 0.001918434645094842 1.0259880607875587 >1k 0.0017385813971136461 2.549994098029403 >5k 1.1990216531818249E-4 0.5069608200931683 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6367 0.2729718432137536 No Hit GTCCTACAGTGGACATTTCTAAATT 5481 0.23498644144095862 No Hit CTGTAGGACGTGGAATATGGCAAGA 4995 0.2141502052540756 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4873 0.20891970975037244 No Hit CTTTAGGACGTGAAATATGGCGAGG 4329 0.1855968445535322 No Hit GGTATCAACGCAGAGTACTTTTTTT 4204 0.1802377303079347 No Hit TATCAACGCAGAGTACTTTTTTTTT 4048 0.17354955572942904 No Hit GTCCTACAGTGTGCATTTCTCATTT 2854 0.12235929645548183 No Hit CTGAAGGACCTGGAATATGGCGAGA 2474 0.10606758914886547 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 8.57458279295598E-5 0.0 7 0.0 0.0 0.0 8.57458279295598E-5 0.0 8 0.0 0.0 0.0 8.57458279295598E-5 0.0 9 0.0 0.0 0.0 8.57458279295598E-5 0.0 10 0.0 0.0 0.0 1.286187418943397E-4 0.0 11 0.0 0.0 0.0 2.572374837886794E-4 0.0 12 0.0 0.0 0.0 2.572374837886794E-4 0.0 13 0.0 0.0 0.0 2.572374837886794E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 190 0.0 13.995799 18 GGTATCA 1315 0.0 13.056437 1 TAGGACC 900 0.0 12.139409 4 ATTGCGA 80 2.8766251E-5 11.871436 17 TTGCGAG 75 2.0804565E-4 11.396579 18 CCAACGT 85 5.3415817E-5 11.173117 19 TGGACCG 95 1.3602461E-5 11.000472 5 GGCGAGG 1225 0.0 10.698829 19 GTCCTAC 2600 0.0 10.492297 1 GCGTGCG 100 2.4077473E-5 10.446864 9 GGACCTG 1720 0.0 10.15974 6 GTCCTAA 1440 0.0 10.134605 1 TCCTACA 2830 0.0 10.071104 2 AGGACCT 1865 0.0 10.035283 5 AAATGTC 815 0.0 10.021531 7 TATGTCG 95 1.6496127E-4 9.996999 16 TAGGACT 390 0.0 9.987609 4 ATTTAGA 940 0.0 9.9443445 1 GAAATGT 890 0.0 9.923986 6 TAAGCTG 240 0.0 9.896257 5 >>END_MODULE