FastQCFastQC Report
Thu 26 May 2016
SRR1511804_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511804_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343720
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72870.3109159797245405No Hit
CTGTAGGACGTGGAATATGGCAAGA67910.28975304217227316No Hit
GTATCAACGCAGAGTACTTTTTTTT62280.26573140136193746No Hit
GTCCTAAAGTGTGTATTTCTCATTT62060.26479272268018367No Hit
CTTTAGGACGTGAAATATGGCGAGG53010.22617889508985714No Hit
GTCCTACAGTGTGCATTTCTCATTT41530.17719693478743193No Hit
GGTATCAACGCAGAGTACTTTTTTT38170.16286075128428312No Hit
TATCAACGCAGAGTACTTTTTTTTT37410.15961804311095182No Hit
CTGTAGGACCTGGAATATGGCGAGA33130.14135647602955984No Hit
CTGAAGGACCTGGAATATGGCGAGA31080.13260969740412676No Hit
GTCCTTCAGTGTGCATTTCTCATTT24890.1061986926765996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11950.013.5460511
TAGGACC12600.013.4932524
TCCAACG2550.012.96493518
CGGTAGG1101.8866558E-812.7174781
GGCGAGG17100.012.09735719
AATGTCC9150.011.0448258
CCAACGA1803.6379788E-1211.02019519
GTCCTAA18050.010.85036851
GTATCAA24950.010.7732651
GTCAACG650.00731926310.7609422
AAATGTC8700.010.7476147
AGGACCT25550.010.646735
GTGGTAT4250.010.5800861
TGTAGGA40150.010.5273842
GTCCTAC37700.010.469391
CTGTAGG39900.010.4681271
GGACCTG25100.010.3860426
CCGGTTT2500.010.20068112
TGGCGAG41100.010.18132718
GTAGGAC42300.010.1598133
Produced by FastQC (version 0.11.5)