FastQCFastQC Report
Thu 26 May 2016
SRR1511804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511804_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343720
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80190.3421483794992576No Hit
GTATCAACGCAGAGTACTTTTTTTT77850.33216425170242175No Hit
CTGTAGGACGTGGAATATGGCAAGA76460.3262335091222501No Hit
GTCCTAAAGTGTGTATTTCTCATTT66510.28377963237929443No Hit
CTTTAGGACGTGAAATATGGCGAGG59060.25199255883808647No Hit
GGTATCAACGCAGAGTACTTTTTTT51020.21768811974126603No Hit
TATCAACGCAGAGTACTTTTTTTTT49050.20928267881828885No Hit
GTCCTACAGTGTGCATTTCTCATTT46610.1988718788933832No Hit
CTGTAGGACCTGGAATATGGCGAGA35940.15334596282832422No Hit
CTGAAGGACCTGGAATATGGCGAGA34490.14715921697131057No Hit
GTCCTTCAGTGTGCATTTCTCATTT25810.11012407625484273No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25390.10833205331694912No Hit
TTTCTAAATTTTCCACCTTTTTCAG24990.1066253648046695No Hit
ATTTAGAAATGTCCACTGTAGGACG24450.104321335313092No Hit
GAATATGGCAAGAAAACTGAAAATC23860.10180396975747956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAA957.413473E-812.9972419
TAGGACC13150.012.9315744
GGTATCA15000.012.52666951
TGGCGAA1701.8189894E-1211.73280318
ACTGTTC8650.011.3087738
TGTAGGA41300.011.2942012
GACGTGT855.34147E-511.1731457
TCCAACG3150.011.15636218
TCGAACT1803.6379788E-1211.08098119
CCCGCCC1201.2814598E-711.08003614
AGGACCT24850.010.9718435
CTGTAGG40050.010.8836441
TATGTCG1752.0008883E-1110.85391216
GGCGAGG17500.010.80056519
GTAGGAC41700.010.7757913
CCAACGA2500.010.63774219
GACGTGG20050.010.5629417
AGGACGT39600.010.5555685
GTCCCGC1355.7159923E-810.55241412
GTCCTAC40150.010.5477081
Produced by FastQC (version 0.11.5)