FastQCFastQC Report
Thu 26 May 2016
SRR1511803_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511803_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310717
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT60160.260352089849168No Hit
CTGTAGGACGTGGAATATGGCAAGA54050.2339100807238619No Hit
GTCCTAAAGTGTGTATTTCTCATTT53360.23092399458696156No Hit
GTATCAACGCAGAGTACTTTTTTTT50790.21980190564227464No Hit
CTTTAGGACGTGAAATATGGCGAGG43780.18946500155579415No Hit
GGTATCAACGCAGAGTACTTTTTTT33280.14402456034209296No Hit
TATCAACGCAGAGTACTTTTTTTTT31780.13753306874013566No Hit
GTCCTACAGTGTGCATTTCTCATTT28390.12286229771971212No Hit
CTGAAGGACCTGGAATATGGCGAGA25380.10983603790511777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1650.013.16617219
TCCAACG2300.012.730618518
GGTATCA11550.012.630421
TAGGACC8250.012.5937314
CGTTAGG751.2969914E-411.9902491
CGTTTCC2000.011.806622514
GGCGAGG13550.011.50165519
CTAATAC2050.011.057913
ATCCCGT2150.010.98290410
GTCCTAC27900.010.8155771
CCGTTTC1951.8189894E-1210.65623513
GACGTGG16250.010.6368587
ATAGGAC3200.010.625963
GTCCTAA15700.010.4373711
AGGACGT32100.010.357465
TGTAGGA30450.010.2707932
TCCTACA30950.010.2662872
GGACGTG31750.010.1741486
TGGCGAG31700.010.10080818
GATATAC5350.010.0852561
Produced by FastQC (version 0.11.5)