FastQCFastQC Report
Thu 26 May 2016
SRR1511803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310717
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62720.27143090218317517No Hit
GTCCTACAGTGGACATTTCTAAATT61170.2647230275278193No Hit
CTGTAGGACGTGGAATATGGCAAGA59790.25875085525401853No Hit
GTCCTAAAGTGTGTATTTCTCATTT53770.23269833562482986No Hit
CTTTAGGACGTGAAATATGGCGAGG50110.21685909611605403No Hit
GGTATCAACGCAGAGTACTTTTTTT43540.18842636289948098No Hit
TATCAACGCAGAGTACTTTTTTTTT41410.17920844482470158No Hit
CTGAAGGACCTGGAATATGGCGAGA29070.12580510724593277No Hit
GTCCTACAGTGTGCATTTCTCATTT27940.12091485023912492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA903.653804E-813.71878319
TCCAACG1500.013.29666818
GGCGAGG12050.013.00503819
GGTATCA16650.012.7163261
TAGGACC8950.012.6316734
TGTCGAG1600.012.46562718
GTCGAGG1700.012.29103819
TTATCCG752.0740554E-411.4003515
ATAGGAC3600.011.3475713
CCAACGT855.3388372E-511.17367119
GGACCTG17900.010.9297496
GCGACTA700.001495539410.85395316
AGGACCT18300.010.6943185
TGGCGAG29850.010.69078818
TGTAGGA33650.010.5025522
AGGACGT35050.010.4354135
CTGTAGG33100.010.3728341
GTATAGG2300.010.366571
GTAGGAC33400.010.3536123
GGACGTG34900.010.2047386
Produced by FastQC (version 0.11.5)