##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511803_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2310717 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.91411540227557 31.0 31.0 34.0 28.0 34.0 2 31.115902985956307 31.0 31.0 34.0 27.0 34.0 3 31.231691288894314 33.0 31.0 34.0 27.0 34.0 4 34.819865002940645 37.0 35.0 37.0 32.0 37.0 5 34.46873978942467 37.0 35.0 37.0 31.0 37.0 6 34.40802357017324 37.0 35.0 37.0 30.0 37.0 7 34.2495177903655 37.0 35.0 37.0 30.0 37.0 8 34.16277674851572 37.0 35.0 37.0 30.0 37.0 9 35.67688816934311 39.0 35.0 39.0 30.0 39.0 10 35.47943949864912 39.0 35.0 39.0 30.0 39.0 11 35.46461379736246 39.0 35.0 39.0 30.0 39.0 12 35.2951932235752 39.0 35.0 39.0 30.0 39.0 13 35.28661060614519 39.0 35.0 39.0 30.0 39.0 14 36.311896264233134 40.0 36.0 41.0 29.0 41.0 15 36.256784798830836 40.0 36.0 41.0 29.0 41.0 16 36.15493286283002 40.0 36.0 41.0 29.0 41.0 17 36.097470611935606 39.0 36.0 41.0 28.0 41.0 18 36.025119043136826 39.0 36.0 41.0 28.0 41.0 19 36.045089467901086 39.0 36.0 41.0 28.0 41.0 20 35.915666003236225 39.0 36.0 41.0 27.0 41.0 21 35.800297483421815 39.0 36.0 41.0 27.0 41.0 22 35.89028340554036 39.0 36.0 41.0 27.0 41.0 23 35.78467549249865 39.0 36.0 41.0 27.0 41.0 24 35.66584441106375 39.0 36.0 41.0 27.0 41.0 25 35.22035454796065 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38741779499505213 1101 2 0.31937161373311085 1101 3 0.2993633112848144 1101 4 0.3261644729401425 1101 5 0.410947781981406 1101 6 0.3579932012977096 1101 7 0.3344763360289704 1101 8 0.2774521341957481 1101 9 0.310852161628965 1101 10 0.3962387405308263 1101 11 0.3113432717934401 1101 12 0.4584134739597516 1101 13 0.3688199536398713 1101 14 0.35569121567988304 1101 15 0.09019111690400905 1101 16 0.4167567588775114 1101 17 0.3297843610064106 1101 18 0.42742812876005587 1101 19 0.4682294060511083 1101 20 0.6168702395436299 1101 21 0.5361223397109782 1101 22 0.4176829082669826 1101 23 0.30468986562405576 1101 24 0.2538106956609738 1101 25 0.3989873474156056 1102 1 0.5289297449751729 1102 2 0.5167817055991826 1102 3 0.5074361986167517 1102 4 0.5176426515760824 1102 5 0.4677860450701985 1102 6 0.5350371913119005 1102 7 0.6248696018849174 1102 8 0.5200114188220581 1102 9 0.5359033306695693 1102 10 0.6248836525450088 1102 11 0.5412577815390449 1102 12 0.7091669288996059 1102 13 0.8427661519037102 1102 14 0.9297704512230993 1102 15 0.8987841764364148 1102 16 0.9549508802148168 1102 17 1.0063089802495355 1102 18 0.8925663953662308 1102 19 0.9993099510409067 1102 20 0.9248034555417632 1102 21 0.9822210927313648 1102 22 0.7953053076842522 1102 23 1.0429935601690374 1102 24 1.0508313373654872 1102 25 1.1470406531560187 1103 1 0.5065833799044483 1103 2 0.4545716710338219 1103 3 0.4394003268373581 1103 4 0.5128272900836635 1103 5 0.6609234645496116 1103 6 0.6658417187302135 1103 7 0.6367004525545639 1103 8 0.5219749642849862 1103 9 0.9016874963606227 1103 10 0.7632157565785604 1103 11 0.7272535926420574 1103 12 0.7608299192641041 1103 13 0.6641087386171804 1103 14 0.9848782353000729 1103 15 0.9112634537473099 1103 16 0.8436824341947045 1103 17 0.855961413132377 1103 18 0.9173381631051143 1103 19 1.0659585116294394 1103 20 0.9617011493869754 1103 21 0.919694455486713 1103 22 1.066567047642124 1103 23 1.0846653005965905 1103 24 1.1275713909096154 1103 25 1.0184708274788505 1104 1 0.5032844352673393 1104 2 0.4413798428192912 1104 3 0.5129994136864902 1104 4 0.4755683644081614 1104 5 0.5546438890233816 1104 6 0.5091804162011684 1104 7 0.4524036535725031 1104 8 0.6303500879928094 1104 9 0.6933803599784625 1104 10 0.6288713456773039 1104 11 0.6652778435339997 1104 12 0.7269005013354075 1104 13 0.7066846058194329 1104 14 0.853350204562318 1104 15 0.9043570226037048 1104 16 1.009087488958805 1104 17 0.9635140357382639 1104 18 0.9297108168146835 1104 19 0.9745795956010639 1104 20 0.9888623978588171 1104 21 0.9701221311102444 1104 22 0.9418531349819261 1104 23 0.946331468273705 1104 24 0.9978311981333192 1104 25 1.1879097968991061 1105 1 0.14975829410049357 1105 2 0.2154926894365694 1105 3 0.205247205587348 1105 4 0.19038042979516234 1105 5 0.08913366536023659 1105 6 0.11452025389515086 1105 7 0.1637373000039446 1105 8 0.2292542446226662 1105 9 0.3102018082087028 1105 10 0.2605457640470732 1105 11 0.4352386052713939 1105 12 0.2751847742155533 1105 13 0.44238334484205666 1105 14 0.5618599080786097 1105 15 0.5320338851586897 1105 16 0.5103737161771278 1105 17 0.40213317209249055 1105 18 0.4422756457974728 1105 19 0.4881695683817284 1105 20 0.6310336827225456 1105 21 0.5834409205315652 1105 22 0.6285005107652992 1105 23 0.8157806681060507 1105 24 0.7837215834964866 1105 25 0.9426398507822782 1106 1 0.30106322381049466 1106 2 0.32547130472416086 1106 3 0.4120854709052644 1106 4 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0.6195669209408621 1107 17 0.7166867943219302 1107 18 0.6606700579872822 1107 19 0.7375510699349235 1107 20 0.7499101089287237 1107 21 0.7360735524608231 1107 22 0.6980557481662331 1107 23 0.6758010260983198 1107 24 0.6270944519336936 1107 25 0.49965403794112717 1108 1 0.010675571627722036 1108 2 0.01787035287686578 1108 3 0.09069292122072525 1108 4 0.08976964944987031 1108 5 0.11544486973821932 1108 6 0.2360236687781665 1108 7 0.20962235393454876 1108 8 0.2665335045628865 1108 9 0.20236658894866366 1108 10 0.10760328521260476 1108 11 0.2055563169387895 1108 12 0.17680533956038857 1108 13 0.3198403038393991 1108 14 0.34634830132682026 1108 15 0.4836007846181758 1108 16 0.4786314279407904 1108 17 0.3059542793905692 1108 18 0.35409417328258286 1108 19 0.46463936387379334 1108 20 0.2960045394996058 1108 21 0.2580725992870967 1108 22 0.32881191729280346 1108 23 0.34459804526411375 1108 24 0.4063132366464117 1108 25 0.4132834082288426 1109 1 0.10386401880716534 1109 2 0.10994653552521783 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2315 5 -0.5538408725290722 2315 6 -0.7760386294047095 2315 7 -0.7753604933587965 2315 8 -0.830081284260153 2315 9 -0.9621897071859635 2315 10 -0.9692654644026462 2315 11 -0.8944531967973646 2315 12 -0.9147615727845206 2315 13 -0.9038780000293372 2315 14 -1.0645119083111538 2315 15 -1.156374815446739 2315 16 -1.1757412530860236 2315 17 -1.2396708010564623 2315 18 -1.3080663402353139 2315 19 -1.256616001593585 2315 20 -1.2823198117475343 2315 21 -1.2666323349841804 2315 22 -1.3465447079051671 2315 23 -1.3138588267313622 2315 24 -1.1129822991728062 2315 25 -1.0910945892975832 2316 1 -1.6528617828243526 2316 2 -1.1657597533147062 2316 3 -1.034280959175213 2316 4 -0.9892454705635743 2316 5 -1.0710117033536406 2316 6 -1.0044435001463015 2316 7 -0.9813057988564893 2316 8 -1.056451988299365 2316 9 -1.419708196363878 2316 10 -1.2491761092951563 2316 11 -1.2366581107342398 2316 12 -1.321818103015488 2316 13 -1.262727806195116 2316 14 -1.5278112667325487 2316 15 -1.3764911145645726 2316 16 -1.4690185315627673 2316 17 -1.5965994497811096 2316 18 -1.635316267642068 2316 19 -1.4404778264852354 2316 20 -1.5149910296182156 2316 21 -2.0435418697268943 2316 22 -1.826090243777685 2316 23 -1.7572760514077785 2316 24 -1.5667639864958431 2316 25 -1.3980287329030787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33894.0 3 3.0 4 627.0 5 5584.0 6 7173.0 7 5577.0 8 4519.0 9 4181.0 10 3990.0 11 3694.0 12 3575.0 13 3476.0 14 3361.0 15 3391.0 16 3591.0 17 3534.0 18 4014.0 19 4420.0 20 5480.0 21 6887.0 22 8957.0 23 11258.0 24 13874.0 25 16514.0 26 18955.0 27 21928.0 28 25941.0 29 31451.0 30 40370.0 31 51778.0 32 66371.0 33 87181.0 34 114236.0 35 155177.0 36 228645.0 37 412457.0 38 894653.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.7385889700112 20.02688884582193 13.286774668169569 24.9477475159973 2 14.635543859330241 21.166763389891535 41.47600073916451 22.72169201161371 3 18.624652001954374 25.582968403313778 30.443667485027376 25.348712109704476 4 12.345908218098538 17.794087289789275 39.05510713774123 30.804897354370958 5 12.446901120611997 38.58213384229184 36.538479020396295 12.432486016699876 6 30.830170895007914 38.43993011692907 18.332491603255612 12.3974073848074 7 27.27066966660132 31.86456844347447 23.02938871354649 17.835373176377722 8 24.15025293015112 38.444863650546566 20.888797719495724 16.516085699806595 9 26.25514072039112 14.960940695031022 20.641818102346587 38.14210048223127 10 14.31053651312558 29.66122636393812 35.61305863071939 20.415178492216917 11 33.91189834151045 22.15636964630459 25.731839944052 18.199892068132964 12 23.042112037086323 24.9040449349704 32.87157189738077 19.182271130562505 13 28.985288981731642 22.38560585307504 26.16304809286468 22.46605707232863 14 19.99011562212075 23.05076735922227 28.434853770496343 28.524263248160636 15 22.166409819982285 32.658953909111325 23.553381915656484 21.62125435524991 16 21.12283091842215 28.725938703768055 29.094959001831906 21.056271375977893 17 20.91208053604141 29.41757904581132 28.614365151595806 21.055975266551464 18 20.615099921496128 27.810148724593354 31.3048694040024 20.26988194990812 19 22.98351550622599 27.18346729608169 28.26720018072313 21.56581701696919 20 23.11403776403601 27.81768602559292 28.994203963531668 20.0740722468394 21 22.17753190892697 27.21306849778662 27.82149436733274 22.787905225953676 22 22.50310185107047 28.991607366890882 28.124517195312105 20.380773586726544 23 21.371851247902708 28.611335788848223 28.769425247661225 21.247387715587845 24 22.550764755092708 27.224337826088274 29.611197740509105 20.613699678309917 25 21.994187946155193 28.087063960722112 29.041833371921676 20.876914721201015 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 232.0 1 232.0 2 404.5 3 577.0 4 577.0 5 577.0 6 1498.5 7 2420.0 8 2420.0 9 2420.0 10 3604.0 11 4788.0 12 4788.0 13 4788.0 14 7971.5 15 11155.0 16 11155.0 17 11155.0 18 20618.0 19 30081.0 20 30081.0 21 30081.0 22 48471.0 23 66861.0 24 66861.0 25 66861.0 26 97141.5 27 127422.0 28 127422.0 29 127422.0 30 164728.0 31 202034.0 32 202034.0 33 202034.0 34 226336.5 35 250639.0 36 250639.0 37 250639.0 38 263806.0 39 276973.0 40 276973.0 41 276973.0 42 286130.5 43 295288.0 44 295288.0 45 295288.0 46 297344.0 47 299400.0 48 299400.0 49 299400.0 50 281783.5 51 264167.0 52 264167.0 53 264167.0 54 229136.5 55 194106.0 56 194106.0 57 194106.0 58 163611.5 59 133117.0 60 133117.0 61 133117.0 62 106825.0 63 80533.0 64 80533.0 65 80533.0 66 61185.5 67 41838.0 68 41838.0 69 41838.0 70 30183.5 71 18529.0 72 18529.0 73 18529.0 74 12752.5 75 6976.0 76 6976.0 77 6976.0 78 4742.5 79 2509.0 80 2509.0 81 2509.0 82 1653.5 83 798.0 84 798.0 85 798.0 86 513.0 87 228.0 88 228.0 89 228.0 90 133.5 91 39.0 92 39.0 93 39.0 94 22.5 95 6.0 96 6.0 97 6.0 98 3.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4064106508932076 2 0.0 3 0.0 4 0.0 5 0.027697030835017875 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.7310644271886173E-4 17 0.0 18 1.2982983203914627E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004673873953409267 25 8.655322135943086E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2310717.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.82578641566464 #Duplication Level Percentage of deduplicated Percentage of total 1 84.71159700853761 60.84477073665078 2 10.86237257194225 15.603969046393956 3 2.4486893550263718 5.276371158373074 4 0.8199937146167497 2.355867736330005 5 0.35883827011448083 1.2886920473504637 6 0.194111149894627 0.8365311595938325 7 0.11539687097513286 0.5801929705387131 8 0.08031481631345104 0.4614939874034618 9 0.05646363280758386 0.3649990347261029 >10 0.30779249119621255 4.018984138257842 >50 0.022209497391953215 1.1238702680345614 >100 0.017889411652887925 2.5983500144948346 >500 0.002165604765361664 1.0888238573628004 >1k 0.0018648263257248603 2.3146148428115065 >5k 3.0077843963304197E-4 1.242469001677948 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6272 0.27143090218317517 No Hit GTCCTACAGTGGACATTTCTAAATT 6117 0.2647230275278193 No Hit CTGTAGGACGTGGAATATGGCAAGA 5979 0.25875085525401853 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5377 0.23269833562482986 No Hit CTTTAGGACGTGAAATATGGCGAGG 5011 0.21685909611605403 No Hit GGTATCAACGCAGAGTACTTTTTTT 4354 0.18842636289948098 No Hit TATCAACGCAGAGTACTTTTTTTTT 4141 0.17920844482470158 No Hit CTGAAGGACCTGGAATATGGCGAGA 2907 0.12580510724593277 No Hit GTCCTACAGTGTGCATTTCTCATTT 2794 0.12091485023912492 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.327661067971543E-5 0.0 4 0.0 4.327661067971543E-5 0.0 8.655322135943086E-5 0.0 5 0.0 4.327661067971543E-5 0.0 8.655322135943086E-5 0.0 6 0.0 4.327661067971543E-5 0.0 8.655322135943086E-5 0.0 7 0.0 4.327661067971543E-5 0.0 1.7310644271886173E-4 0.0 8 0.0 4.327661067971543E-5 0.0 1.7310644271886173E-4 0.0 9 0.0 4.327661067971543E-5 0.0 2.596596640782926E-4 0.0 10 0.0 4.327661067971543E-5 0.0 3.02936274758008E-4 0.0 11 0.0 4.327661067971543E-5 0.0 3.02936274758008E-4 0.0 12 0.0 4.327661067971543E-5 0.0 3.02936274758008E-4 0.0 13 0.0 4.327661067971543E-5 0.0 3.02936274758008E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 90 3.653804E-8 13.718783 19 TCCAACG 150 0.0 13.296668 18 GGCGAGG 1205 0.0 13.005038 19 GGTATCA 1665 0.0 12.716326 1 TAGGACC 895 0.0 12.631673 4 TGTCGAG 160 0.0 12.465627 18 GTCGAGG 170 0.0 12.291038 19 TTATCCG 75 2.0740554E-4 11.400351 5 ATAGGAC 360 0.0 11.347571 3 CCAACGT 85 5.3388372E-5 11.173671 19 GGACCTG 1790 0.0 10.929749 6 GCGACTA 70 0.0014955394 10.853953 16 AGGACCT 1830 0.0 10.694318 5 TGGCGAG 2985 0.0 10.690788 18 TGTAGGA 3365 0.0 10.502552 2 AGGACGT 3505 0.0 10.435413 5 CTGTAGG 3310 0.0 10.372834 1 GTATAGG 230 0.0 10.36657 1 GTAGGAC 3340 0.0 10.353612 3 GGACGTG 3490 0.0 10.204738 6 >>END_MODULE