FastQCFastQC Report
Thu 26 May 2016
SRR1511802_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511802_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658373
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59950.22551387634466646No Hit
GTCCTACAGTGGACATTTCTAAATT48120.1810129729725663No Hit
GTCCTAAAGTGTGTATTTCTCATTT44360.16686898339698755No Hit
CTGTAGGACGTGGAATATGGCAAGA44360.16686898339698755No Hit
GGTATCAACGCAGAGTACTTTTTTT37820.14226746961393302No Hit
CTTTAGGACGTGAAATATGGCGAGG37120.13963428006528805No Hit
TATCAACGCAGAGTACTTTTTTTTT36340.1367001545682265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.013.2355951
TAGGCGT500.001567034313.22308354
TCCAACG1700.012.77909118
TAGGACC8700.012.7019774
CGTTAGG801.7064265E-512.4919291
GGCGAGG11100.011.913136519
CGAGTAA803.0434896E-511.80632610
TTGCGAG658.424138E-411.62512718
GGCGAGT855.6499463E-511.1118358
ACTGCGC600.006136738711.0192378
GTCCTAA14600.010.6095821
GCGAGTA901.0096372E-410.4945119
TGGCGAG25500.010.40847718
GTAGGAC26150.010.3660893
AGGACCT17450.010.284025
GGACGTG27800.010.2604616
CCAACGA1201.6417052E-610.23253319
AGGACGT28200.010.2154035
GTCCTAC28750.010.1499621
TGTCGAG1401.0548683E-710.12008918
Produced by FastQC (version 0.11.5)