FastQCFastQC Report
Thu 26 May 2016
SRR1511802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511802_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658373
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72800.2738517130590779No Hit
GTCCTACAGTGGACATTTCTAAATT53270.20038572465188292No Hit
CTGTAGGACGTGGAATATGGCAAGA49140.18484990631487758No Hit
GGTATCAACGCAGAGTACTTTTTTT48300.18169007885650357No Hit
TATCAACGCAGAGTACTTTTTTTTT47100.17717603963025505No Hit
GTCCTAAAGTGTGTATTTCTCATTT46590.1752575729590994No Hit
CTTTAGGACGTGAAATATGGCGAGG41950.15780328795093843No Hit
GTCCTACAGTGTGCATTTCTCATTT26930.10130256363572758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.013.0619191
TAGGACC8300.012.2471584
TCCAACG2650.011.46862118
GGCGAGG12350.011.22796719
GTATACG855.117637E-511.2203241
TGTAGGA29150.010.5267322
CTAAGAC2650.010.3963713
GTAGGAC28100.010.3791483
GTCCTAC27300.010.3407821
ACGAAAT1951.4551915E-1110.22783312
AATGTCC6700.010.2060248
CTGTAGG29250.010.1404881
ACCGCCC750.00265494510.13042458
TGGCGAG28700.010.0931118
AGGACGT29850.010.0889185
GTATCAA28250.010.0605591
CTAGGAC4350.010.0461163
GGACGTG29200.010.0176726
TACACCG951.64459E-410.0001395
CCAACGA1901.0004442E-109.99750519
Produced by FastQC (version 0.11.5)