##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511802_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2658373 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27011183156013 31.0 31.0 34.0 28.0 34.0 2 31.46245842852 33.0 31.0 34.0 28.0 34.0 3 31.581073084928263 34.0 31.0 34.0 28.0 34.0 4 35.142399129091366 37.0 35.0 37.0 32.0 37.0 5 34.832332031659966 37.0 35.0 37.0 32.0 37.0 6 34.78392836520684 37.0 35.0 37.0 32.0 37.0 7 34.64576077172015 37.0 35.0 37.0 32.0 37.0 8 34.571974662697826 37.0 35.0 37.0 31.0 37.0 9 36.14484649069186 39.0 37.0 39.0 32.0 39.0 10 35.972026498914936 39.0 37.0 39.0 31.0 39.0 11 35.958124762777835 39.0 37.0 39.0 31.0 39.0 12 35.80628226362516 39.0 35.0 39.0 30.0 39.0 13 35.7975246513563 39.0 35.0 39.0 30.0 39.0 14 36.905034395098056 40.0 37.0 41.0 31.0 41.0 15 36.853983996978606 40.0 37.0 41.0 31.0 41.0 16 36.764634609213985 40.0 37.0 41.0 30.0 41.0 17 36.712255202712335 40.0 37.0 41.0 30.0 41.0 18 36.64839847530802 40.0 36.0 41.0 30.0 41.0 19 36.66706177048894 40.0 37.0 41.0 30.0 41.0 20 36.544917887745626 40.0 36.0 41.0 30.0 41.0 21 36.4477603406294 40.0 36.0 41.0 30.0 41.0 22 36.53402174939333 40.0 37.0 41.0 30.0 41.0 23 36.44894264273674 40.0 37.0 41.0 30.0 41.0 24 36.34501516529095 40.0 37.0 41.0 30.0 41.0 25 35.92561465227039 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19689076997268984 1101 2 0.11418711694860306 1101 3 0.11773471681178549 1101 4 0.1219152246081805 1101 5 0.10616401984960788 1101 6 0.21967797804481393 1101 7 0.22736320912167685 1101 8 0.15521270932414666 1101 9 0.14080356332917177 1101 10 0.07555595515012214 1101 11 -0.054570470715084696 1101 12 0.18409288407407587 1101 13 0.14433425837741254 1101 14 0.09994692461034305 1101 15 -0.043688232232732105 1101 16 -0.015155028773115475 1101 17 0.017115047440285025 1101 18 0.09739770140680548 1101 19 0.01563162549821584 1101 20 0.18082742586235412 1101 21 0.18050191415502326 1101 22 0.05599556925947269 1101 23 0.00730371815487274 1101 24 -0.030083369801033655 1101 25 -0.07357086299659699 1102 1 0.3893923314127825 1102 2 0.4343069489659257 1102 3 0.45990777360002966 1102 4 0.4225009086551026 1102 5 0.5181469771979437 1102 6 0.5108550472715123 1102 7 0.5631052972724291 1102 8 0.49788972112057195 1102 9 0.5562344317691625 1102 10 0.6254780489775769 1102 11 0.6967478944388148 1102 12 0.7940301367005915 1102 13 0.8744130233856566 1102 14 0.9073432455118535 1102 15 0.9462375552864302 1102 16 1.013177646468641 1102 17 1.0147611044942053 1102 18 1.14023806654464 1102 19 1.0308944623861223 1102 20 1.010362505983693 1102 21 1.016115526072312 1102 22 0.8187991161810473 1102 23 1.0216111876164575 1102 24 0.8895582373668276 1102 25 0.961178292394365 1103 1 0.3373548374821702 1103 2 0.35890124845393956 1103 3 0.4008069923290236 1103 4 0.3747513713236472 1103 5 0.48503134221662947 1103 6 0.4593253914002702 1103 7 0.43467092543153285 1103 8 0.4026922869532257 1103 9 0.6090745990545869 1103 10 0.5200255184809208 1103 11 0.4745603220272301 1103 12 0.5538441709967614 1103 13 0.5344007263890305 1103 14 0.7593971740007746 1103 15 0.9185910895160703 1103 16 0.4463012435341085 1103 17 0.5004364984897691 1103 18 0.6909725347811957 1103 19 0.699705107352365 1103 20 0.556208628840011 1103 21 0.4365666495938356 1103 22 0.7901737223726286 1103 23 0.7949954714831264 1103 24 0.7936379208161384 1103 25 0.6779576021361109 1104 1 0.37026999973041796 1104 2 0.2849790918270756 1104 3 0.3414962788673357 1104 4 0.29421640373335123 1104 5 0.3260345371556497 1104 6 0.3236063239126423 1104 7 0.3642353229288986 1104 8 0.42046181132346305 1104 9 0.5614895407242599 1104 10 0.4745366466985317 1104 11 0.5538242634132402 1104 12 0.5576155207216971 1104 13 0.5987528415646537 1104 14 0.62415758280099 1104 15 0.680018269440481 1104 16 0.7081068249108782 1104 17 0.8649071594282702 1104 18 0.6752691586543804 1104 19 0.7986722280737126 1104 20 0.9302220532018595 1104 21 0.7627802011557279 1104 22 0.8535004759641893 1104 23 0.8164957532749 1104 24 0.7632855942357892 1104 25 0.975614468251166 1105 1 0.24003657903737619 1105 2 0.18758152789657245 1105 3 0.2967279723392622 1105 4 0.3451559092564267 1105 5 0.3358103715220224 1105 6 0.40767669714576016 1105 7 0.2884205132844784 1105 8 0.3453530675373102 1105 9 0.5695068505627532 1105 10 0.49572984740763104 1105 11 0.5390686569009304 1105 12 0.6186748819518542 1105 13 0.5667319802117348 1105 14 0.6976708011411858 1105 15 0.6913211728003645 1105 16 0.5593138007024265 1105 17 0.6126520884730766 1105 18 0.6593181728419353 1105 19 0.7315736797141312 1105 20 0.709281145349145 1105 21 0.7422118039109975 1105 22 0.7461204435256334 1105 23 0.7914765799347805 1105 24 0.8850411654922397 1105 25 0.8762996493580033 1106 1 0.1746771430989824 1106 2 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0.5222873666903922 1107 15 0.5272506582678034 1107 16 0.7029371156841151 1107 17 0.6838896386380355 1107 18 0.6952532586141018 1107 19 0.7626637409842019 1107 20 0.7361082790081781 1107 21 0.5219566887250551 1107 22 0.5134475656949107 1107 23 0.6402759613445994 1107 24 0.6192260495088675 1107 25 0.48973328901887214 1108 1 0.15402630534162398 1108 2 0.1356645214161425 1108 3 0.18238857073880155 1108 4 0.12103694667690235 1108 5 0.06330111826878948 1108 6 0.23215346355557642 1108 7 0.2433574333384172 1108 8 0.33723209833718215 1108 9 0.24660424597573183 1108 10 0.21126779245785343 1108 11 0.28120366977531575 1108 12 0.21228437085840568 1108 13 0.32510492655169543 1108 14 0.4626449181383805 1108 15 0.45195750055413697 1108 16 0.4046519751612436 1108 17 0.37144243800449317 1108 18 0.40698637379413327 1108 19 0.3829714093314962 1108 20 0.30978297849674163 1108 21 0.3526764312154853 1108 22 0.5713127582636588 1108 23 0.5457581146791455 1108 24 0.5532476059315172 1108 25 0.41048688420722357 1109 1 0.08133837190978355 1109 2 -0.006320352155583464 1109 3 -0.05158152364715818 1109 4 -0.05177489105163602 1109 5 -0.028028290815704793 1109 6 0.016565706591009643 1109 7 0.05878660260770374 1109 8 0.06892326057964482 1109 9 0.1798432655208586 1109 10 0.05043514033260976 1109 11 0.04695224087240035 1109 12 0.23019867985058795 1109 13 0.23102971436949815 1109 14 0.18525439518980136 1109 15 0.1340948421826127 1109 16 0.1241392654727278 1109 17 0.2191116441713561 1109 18 0.08618686085961969 1109 19 0.12341471681773442 1109 20 0.3354006241898233 1109 21 0.2322427893265413 1109 22 0.23562934211639686 1109 23 0.19897832593603937 1109 24 0.18593584439206978 1109 25 0.10484811527587112 1110 1 -0.06522299750982086 1110 2 -0.09030280120388312 1110 3 0.048267316857291576 1110 4 -0.012992847017159193 1110 5 0.06408471687477402 1110 6 0.12172066722355623 1110 7 0.016203818287081617 1110 8 0.1568841757141186 1110 9 0.06533257545944338 1110 10 0.12521908910103008 1110 11 0.08904191543979323 1110 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2313 12 -0.4885950187570174 2313 13 -0.334448704723016 2313 14 -0.3502363197867808 2313 15 -0.2184371597348047 2313 16 -0.25765608960460895 2313 17 -0.41924754313736656 2313 18 -0.3197489169011405 2313 19 -0.26871584646706737 2313 20 -0.2749102617094792 2313 21 -0.2157805090337206 2313 22 -0.31301846518852017 2313 23 -0.288975395022554 2313 24 -0.24416269286573566 2313 25 -0.18631473215022254 2314 1 -0.2590972813398764 2314 2 -0.2983021675122828 2314 3 -0.4524923877254494 2314 4 -0.44839965134979565 2314 5 -0.3783839954659669 2314 6 -0.402589015927191 2314 7 -0.41633272231172214 2314 8 -0.3697377218997886 2314 9 -0.45904077619690753 2314 10 -0.5735511646507732 2314 11 -0.4934444329618657 2314 12 -0.6684736071255699 2314 13 -0.611974279038968 2314 14 -0.6043866153515438 2314 15 -0.5633674276899256 2314 16 -0.6104444864755152 2314 17 -0.5316329454240076 2314 18 -0.5529619005123365 2314 19 -0.7000412862745122 2314 20 -0.6871567308610125 2314 21 -0.5958643998694484 2314 22 -0.49837916038515573 2314 23 -0.6080614450738793 2314 24 -0.6129372495185947 2314 25 -0.6009018264123682 2315 1 -0.4997322734241294 2315 2 -0.5260571239099079 2315 3 -0.5813397006833476 2315 4 -0.5861607282310857 2315 5 -0.5837702437268675 2315 6 -0.5972884018143745 2315 7 -0.6261676554579836 2315 8 -0.7383216880946364 2315 9 -0.7607090735083091 2315 10 -0.8159900195212373 2315 11 -0.8256579714555343 2315 12 -0.7303743856656624 2315 13 -0.8211064899867822 2315 14 -0.8377178100238041 2315 15 -1.0437768200416642 2315 16 -0.9386343736716043 2315 17 -1.0854767063154966 2315 18 -1.1081334865717238 2315 19 -1.008649572486462 2315 20 -1.019570338736358 2315 21 -1.0362448373140154 2315 22 -1.1867593088068844 2315 23 -1.202200312857265 2315 24 -1.0524155025738793 2315 25 -1.2983845751086278 2316 1 -1.5747197161192936 2316 2 -1.0948363127468426 2316 3 -1.009786972059679 2316 4 -0.8552058376666167 2316 5 -0.8601510057315096 2316 6 -0.7891938548112236 2316 7 -1.0250382336825368 2316 8 -0.9819363649989654 2316 9 -1.1923400150343824 2316 10 -1.3281372961697997 2316 11 -1.1039213074358827 2316 12 -1.168887833731958 2316 13 -1.2202639755504805 2316 14 -1.4449284821594262 2316 15 -1.4800300554123567 2316 16 -1.5081160154845463 2316 17 -1.5373152020055514 2316 18 -1.5936178961004757 2316 19 -1.4428322319213223 2316 20 -1.3014613973222637 2316 21 -1.623936001589719 2316 22 -1.60760966619452 2316 23 -1.3684890683374178 2316 24 -1.415470174769979 2316 25 -1.4071892790811518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29318.0 3 0.0 4 553.0 5 4924.0 6 6361.0 7 5158.0 8 4175.0 9 3910.0 10 3656.0 11 3634.0 12 3378.0 13 3309.0 14 3316.0 15 3268.0 16 3370.0 17 3567.0 18 3826.0 19 4405.0 20 5154.0 21 6774.0 22 8832.0 23 11075.0 24 13776.0 25 16250.0 26 19350.0 27 22110.0 28 26079.0 29 32066.0 30 41014.0 31 53479.0 32 69535.0 33 91283.0 34 121072.0 35 164675.0 36 243601.0 37 446899.0 38 1175221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.207953112641015 19.423834860763492 13.26114334021061 25.10706868638488 2 14.823089160174288 20.833795708879077 41.0099335194873 23.333181611459338 3 18.594079912788764 25.20537938054592 30.085770506998077 26.11477019966724 4 12.369295053779135 17.47907460691182 38.66086512314111 31.490765216167933 5 12.87956180622109 38.08830927624803 36.11881532353741 12.913313593993461 6 31.177340425892076 37.92770991881124 18.20624870926691 12.68870094602977 7 27.450211087759318 31.65778466753913 22.75177335911853 18.140230885583026 8 24.583194307194663 37.52750272441076 20.867876705037254 17.021426263357327 9 26.27607186801852 15.037581257408197 20.41515618763808 38.2711906869352 10 14.538065200030244 29.3461075627837 35.09928817363102 21.01653906355504 11 34.16382877797811 22.2971343750482 25.030911764451414 18.50812508252228 12 23.03074098330069 24.74257750887479 32.39522820913393 19.83145329869059 13 28.923142087284216 22.10419681511962 26.01941112101274 22.95324997658342 14 20.58394363770622 22.4751379885366 28.070552928426522 28.87036544533066 15 22.642947396772385 31.35101808512199 23.59635009834963 22.409684419755994 16 21.768218205811003 28.308802646432724 28.228189368602052 21.694789779154227 17 21.38330475068773 28.763119396713705 28.181598293392234 21.671977559206326 18 21.16993540023977 27.634312617999985 30.328633835257634 20.867118146502612 19 23.246474441321816 26.90122868386039 27.89980939469367 21.952487480124123 20 23.191929800671314 27.483727829014214 28.570144219791576 20.754198150522896 21 22.715435343347227 26.828703120291998 27.574986655371536 22.880874880989236 22 22.843445972404925 28.203152830697576 27.744789764265587 21.208611432631912 23 22.051495407153173 27.85312670569555 28.197322196697 21.898055690454274 24 22.77946017116524 26.978688987115586 28.82324837769209 21.418602464027085 25 22.510034344353873 27.470743350248462 28.4999830723338 21.51923923306387 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 262.0 1 262.0 2 443.5 3 625.0 4 625.0 5 625.0 6 1779.5 7 2934.0 8 2934.0 9 2934.0 10 4156.0 11 5378.0 12 5378.0 13 5378.0 14 8747.0 15 12116.0 16 12116.0 17 12116.0 18 22396.5 19 32677.0 20 32677.0 21 32677.0 22 52012.5 23 71348.0 24 71348.0 25 71348.0 26 103138.5 27 134929.0 28 134929.0 29 134929.0 30 174392.5 31 213856.0 32 213856.0 33 213856.0 34 240496.5 35 267137.0 36 267137.0 37 267137.0 38 285427.0 39 303717.0 40 303717.0 41 303717.0 42 318435.0 43 333153.0 44 333153.0 45 333153.0 46 337903.0 47 342653.0 48 342653.0 49 342653.0 50 328941.5 51 315230.0 52 315230.0 53 315230.0 54 279059.5 55 242889.0 56 242889.0 57 242889.0 58 207549.5 59 172210.0 60 172210.0 61 172210.0 62 140034.5 63 107859.0 64 107859.0 65 107859.0 66 82878.0 67 57897.0 68 57897.0 69 57897.0 70 42062.0 71 26227.0 72 26227.0 73 26227.0 74 18223.0 75 10219.0 76 10219.0 77 10219.0 78 6882.5 79 3546.0 80 3546.0 81 3546.0 82 2329.5 83 1113.0 84 1113.0 85 1113.0 86 720.5 87 328.0 88 328.0 89 328.0 90 191.5 91 55.0 92 55.0 93 55.0 94 33.0 95 11.0 96 11.0 97 11.0 98 7.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41405024802764695 2 0.0 3 0.0 4 0.0 5 0.027686107254324355 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 7.523398710414227E-5 17 0.0 18 1.5046797420828454E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004626890206904749 25 1.1285098065621341E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2658373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.54689515882583 #Duplication Level Percentage of deduplicated Percentage of total 1 80.7031045170037 55.319472443188154 2 12.96297642225197 17.77143571524874 3 3.4683507957525372 7.13234035111438 4 1.2713222117102339 3.485807614367519 5 0.5537207929023532 1.897792056916976 6 0.29835273072009505 1.227069201181185 7 0.17510163047209368 0.8401871174277048 8 0.10548167741120894 0.578435318614658 9 0.07935597781621344 0.4895645302434682 >10 0.3391421297609808 4.014440275375405 >50 0.02176564605288037 1.029849108367363 >100 0.01759780607522314 2.4331553052284556 >500 0.001918649595134688 0.9386589904538043 >1k 0.001699375355569091 2.3680660358415953 >5k 1.0963711971413491E-4 0.4737259364305775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7280 0.2738517130590779 No Hit GTCCTACAGTGGACATTTCTAAATT 5327 0.20038572465188292 No Hit CTGTAGGACGTGGAATATGGCAAGA 4914 0.18484990631487758 No Hit GGTATCAACGCAGAGTACTTTTTTT 4830 0.18169007885650357 No Hit TATCAACGCAGAGTACTTTTTTTTT 4710 0.17717603963025505 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4659 0.1752575729590994 No Hit CTTTAGGACGTGAAATATGGCGAGG 4195 0.15780328795093843 No Hit GTCCTACAGTGTGCATTTCTCATTT 2693 0.10130256363572758 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.7616993552071135E-5 0.0 4 0.0 0.0 0.0 7.523398710414227E-5 0.0 5 0.0 0.0 0.0 7.523398710414227E-5 0.0 6 0.0 0.0 0.0 7.523398710414227E-5 0.0 7 0.0 0.0 0.0 7.523398710414227E-5 0.0 8 0.0 0.0 0.0 1.5046797420828454E-4 0.0 9 0.0 0.0 0.0 2.2570196131242682E-4 3.7616993552071135E-5 10 0.0 0.0 0.0 2.2570196131242682E-4 3.7616993552071135E-5 11 0.0 0.0 0.0 2.2570196131242682E-4 3.7616993552071135E-5 12 0.0 0.0 0.0 2.2570196131242682E-4 3.7616993552071135E-5 13 0.0 0.0 0.0 2.2570196131242682E-4 3.7616993552071135E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1380 0.0 13.061919 1 TAGGACC 830 0.0 12.247158 4 TCCAACG 265 0.0 11.468621 18 GGCGAGG 1235 0.0 11.227967 19 GTATACG 85 5.117637E-5 11.220324 1 TGTAGGA 2915 0.0 10.526732 2 CTAAGAC 265 0.0 10.396371 3 GTAGGAC 2810 0.0 10.379148 3 GTCCTAC 2730 0.0 10.340782 1 ACGAAAT 195 1.4551915E-11 10.227833 12 AATGTCC 670 0.0 10.206024 8 CTGTAGG 2925 0.0 10.140488 1 ACCGCCC 75 0.002654945 10.1304245 8 TGGCGAG 2870 0.0 10.09311 18 AGGACGT 2985 0.0 10.088918 5 GTATCAA 2825 0.0 10.060559 1 CTAGGAC 435 0.0 10.046116 3 GGACGTG 2920 0.0 10.017672 6 TACACCG 95 1.64459E-4 10.000139 5 CCAACGA 190 1.0004442E-10 9.997505 19 >>END_MODULE