FastQCFastQC Report
Thu 26 May 2016
SRR1511801_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511801_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313724
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61170.2643789838373116No Hit
GGTATCAACGCAGAGTACTTTTTTT40230.17387553571644673No Hit
TATCAACGCAGAGTACTTTTTTTTT36200.15645772788802811No Hit
GTCCTAAAGTGTGTATTTCTCATTT24320.10511193210599017No Hit
CTGTAGGACGTGGAATATGGCAAGA24160.10442040623687182No Hit
GTCCTACAGTGGACATTTCTAAATT23800.10286447303135551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14050.013.0908741
TAGGACC4050.012.3597864
ATAATAC2300.011.4979473
GTATTAG2300.011.2998491
TCCAACG2000.010.86215918
GTATCAA28100.010.5296151
GTCCTAA9050.010.38260651
GTATAAT3100.010.3184731
GATTAAG2250.010.2181541
GTATAAG2750.010.1777661
CTAGGAC2900.010.0961043
TAGACCG856.958789E-410.0003165
GACGGTG856.958789E-410.0003167
GATCTAT1501.2625787E-79.996021
TAATACC1804.3837645E-109.9694514
TAGGACT3050.09.9092384
CCCGTTT1252.769968E-69.82253212
TAATACA3850.09.8127194
GTAGGAC14700.09.6374923
GTCCTAG2500.09.5961791
Produced by FastQC (version 0.11.5)