FastQCFastQC Report
Thu 26 May 2016
SRR1511801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511801_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313724
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79410.34321293291680427No Hit
GGTATCAACGCAGAGTACTTTTTTT53450.2310128606523509No Hit
TATCAACGCAGAGTACTTTTTTTTT51410.22219590582109186No Hit
GTCCTAAAGTGTGTATTTCTCATTT27430.11855346618697823No Hit
GTCCTACAGTGGACATTTCTAAATT26360.11392888693724922No Hit
CTGTAGGACGTGGAATATGGCAAGA26180.11315092033449108No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24870.10748905228108452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.013.20307351
ACCGTCT907.488714E-611.6077838
TAGGACC4500.010.55481154
CGCCCTA1451.45082595E-810.479753516
GACGTGA7550.010.4407157
AATCCCG1856.002665E-1110.26843619
TCCTACA13800.010.0500152
GTATCAA32350.09.995961
CTTAGAC2002.5465852E-119.9742973
ACGTGAA8500.09.9442078
GTCCTAC11900.09.9397131
AGGACGT14850.09.9151255
GGACTGT4050.09.8490296
GGCGAGG7550.09.81281819
GGACGTG14900.09.7522376
GGTCTAC2650.09.7188971
AGGACCT10850.09.7182095
TTAGGAC9300.09.6014743
TGGCGAG16250.09.52752918
CCAACGA900.00111576729.49830319
Produced by FastQC (version 0.11.5)