FastQCFastQC Report
Thu 26 May 2016
SRR1511800_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511800_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240365
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT61380.18942310511315855No Hit
GTATCAACGCAGAGTACTTTTTTTT60370.18630617229849106No Hit
CTGTAGGACGTGGAATATGGCAAGA55820.17226454427201873No Hit
GTCCTAAAGTGTGTATTTCTCATTT50440.15566147640775035No Hit
CTTTAGGACGTGAAATATGGCGAGG46870.1446441990331336No Hit
GGTATCAACGCAGAGTACTTTTTTT38310.11822742191080327No Hit
TATCAACGCAGAGTACTTTTTTTTT37600.11603631072425484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.013.0336411
TAGGACC11050.012.9070654
CGCTAAG600.004032352511.65786651
CGACTGT752.1898869E-411.3341086
GGCGAGG14050.011.22724219
TCCAACG3500.011.06493218
GATATAC7050.010.9137491
GCGACTA1002.5577961E-510.39008116
ATAGGAC3550.010.3764573
GTCCTAA17050.010.3734031
CCTACAC5100.010.3712413
CTTATAC2750.010.3038943
TTAGGAC20100.010.2910793
GTCCTAC29650.010.278921
CCAACGA2500.010.20132819
GACGTGA18150.010.19965657
TCCTACA32100.010.1481042
CGTGCGC1502.6780981E-810.07476310
GTATCAA31750.010.0396671
AGGACGT35400.010.0055485
Produced by FastQC (version 0.11.5)