FastQCFastQC Report
Thu 26 May 2016
SRR1511800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240365
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76470.23599193300754698No Hit
GTCCTACAGTGGACATTTCTAAATT68830.21241434221144842No Hit
CTGTAGGACGTGGAATATGGCAAGA62650.19334241667219587No Hit
GTCCTAAAGTGTGTATTTCTCATTT53980.16658617161955522No Hit
CTTTAGGACGTGAAATATGGCGAGG52570.16223480996739564No Hit
TATCAACGCAGAGTACTTTTTTTTT47960.14800801761529953No Hit
GGTATCAACGCAGAGTACTTTTTTT47490.14655756373124632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG350.002170824716.285784
TAGGACC11600.011.9569454
GGTATCA17700.011.7459361
AATCCCG3500.011.39705619
GGCGTGC1701.2732926E-1111.173248
GGCGAGG14200.010.76834519
CCAACGA1701.4006218E-1010.61490519
TTAGGAC20450.010.498823
TATGTCG1909.094947E-1210.49712616
GTATCAA36000.010.4640321
TGTCGAG2100.010.40207618
TGTAGGA34000.010.17062952
GTAGGAC33850.09.9069823
GGACGTG33250.09.8542946
GTCCTAC33500.09.8499881
AGGACGT34150.09.8477715
GACGTGA17800.09.8173857
TAGGACA9650.09.7461544
ATGTCGA2450.09.69107417
AGGACCT22850.09.6871295
Produced by FastQC (version 0.11.5)