FastQCFastQC Report
Thu 26 May 2016
SRR1511799_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511799_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934915
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52420.5606926832920639No Hit
GGTATCAACGCAGAGTACTTTTTTT37950.40591925469160295No Hit
GTCCTACAGTGGACATTTCTAAATT36450.38987501537572933No Hit
GTCCTAAAGTGTGTATTTCTCATTT34570.3697662354331677No Hit
CTGTAGGACGTGGAATATGGCAAGA33120.3542568040944899No Hit
TATCAACGCAGAGTACTTTTTTTTT32510.34773214677270126No Hit
CTTTAGGACGTGAAATATGGCGAGG30800.3294417139526053No Hit
GTCCTACAGTGTGCATTTCTCATTT15490.16568351133525508No Hit
CTGAAGGACCTGGAATATGGCGAGA14780.1580892380590749No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14700.15723354529556163No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12650.13530641823053433No Hit
TTTCTAAATTTTCCACCTTTTTCAG12630.135092495039656No Hit
ATTTAGAAATGTCCACTGTAGGACG12620.13498553344421685No Hit
GAATATGGCAAGAAAACTGAAAATC12340.13199060877192043No Hit
GTCCTTCAGTGTGCATTTCTCATTT11940.12771214495435415No Hit
CTGTAGGACCTGGAATATGGCGAGA10900.11658813902868176No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10320.11038436649321061No Hit
GGAATATGGCGAGAAAACTGAAAAT9470.10129263088088222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCGC350.002253753416.1928819
CGGTAGG651.995355E-615.3706031
GGTATCA8600.014.8701661
TAGGACC6100.014.2454344
ATTGCGA400.00548290214.16801317
GTATAAG851.4577381E-714.1047891
GTCAACG500.001024651213.9872492
GTTATAG500.001024651213.9872491
CCGTAGG602.6713804E-413.321191
TCCAACG1001.089029E-813.22418518
CGTGCGC550.003202279612.02134410
GCGTGCG550.003202279612.0213449
CGTAGGA751.296499E-411.9890712
GTGCTAG709.781264E-411.4181621
CTCTTAC802.362834E-411.2397531
ATAGGAC1605.0931703E-1111.2163433
GTGCGCC600.0061303411.01956611
CTATAGG1551.5879777E-910.9577521
TATAGGA1852.1827873E-1110.8009632
GGCGTGC700.001563916710.7946778
Produced by FastQC (version 0.11.5)