##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511799_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 934915 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.508242995352518 31.0 30.0 34.0 26.0 34.0 2 27.643632843627497 31.0 28.0 34.0 10.0 34.0 3 29.46000331580946 31.0 30.0 34.0 16.0 34.0 4 33.96853617708562 35.0 35.0 37.0 30.0 37.0 5 34.36471550889653 37.0 35.0 37.0 32.0 37.0 6 34.61198076830514 37.0 35.0 37.0 32.0 37.0 7 34.63232486375767 37.0 35.0 37.0 32.0 37.0 8 34.65774963499356 37.0 35.0 37.0 32.0 37.0 9 36.31888888294658 39.0 37.0 39.0 32.0 39.0 10 36.208058486600386 39.0 37.0 39.0 32.0 39.0 11 36.20569249610927 39.0 37.0 39.0 32.0 39.0 12 36.125858500505394 39.0 37.0 39.0 32.0 39.0 13 36.12851649615206 39.0 37.0 39.0 32.0 39.0 14 37.32438136087238 40.0 38.0 41.0 32.0 41.0 15 37.285450548980386 40.0 38.0 41.0 32.0 41.0 16 37.2516838429162 40.0 38.0 41.0 32.0 41.0 17 37.24093313295861 40.0 38.0 41.0 32.0 41.0 18 37.225660086745854 40.0 38.0 41.0 32.0 41.0 19 37.25931020467101 40.0 38.0 41.0 32.0 41.0 20 37.231086248482484 40.0 38.0 41.0 32.0 41.0 21 37.129390372386794 40.0 38.0 41.0 31.0 41.0 22 37.0664541696304 40.0 38.0 41.0 31.0 41.0 23 36.969629324591004 40.0 37.0 41.0 31.0 41.0 24 36.89186396624292 40.0 37.0 41.0 31.0 41.0 25 36.80310937357942 40.0 37.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5264557629366493 1101 2 3.2407390382156294 1101 3 1.8334468305895264 1101 4 0.8183365276636749 1101 5 0.26810603770025665 1101 6 0.3152721492847661 1101 7 1.3515520504370215E-4 1101 8 0.026871389154351277 1101 9 0.027630973405507575 1101 10 0.06396342268438815 1101 11 0.18068941211400613 1101 12 -0.0349278764239358 1101 13 -0.10816400822827887 1101 14 -0.027283471841300866 1101 15 0.1319574724675121 1101 16 -0.004864439203750237 1101 17 0.05414403394770062 1101 18 0.29426348852326356 1101 19 0.29315754457997656 1101 20 0.30531962743352636 1101 21 0.39857060220454343 1101 22 0.45936425494518573 1101 23 0.3073599186897482 1101 24 0.28693390391288176 1101 25 0.27128158310591033 1102 1 1.2305498462345739 1102 2 4.134222604142572 1102 3 2.3545463937275244 1102 4 1.2390722207054736 1102 5 1.0918333094371349 1102 6 0.967212426654001 1102 7 0.9464016737090333 1102 8 0.7485870257636051 1102 9 0.8850625298761869 1102 10 0.8582869402405748 1102 11 0.9491552747802814 1102 12 0.9050417284392864 1102 13 0.9116445786702201 1102 14 1.2326412484471803 1102 15 0.97707300667021 1102 16 1.1669361730567118 1102 17 1.1056932054351023 1102 18 1.0833577352359 1102 19 1.2012885160259827 1102 20 1.151418373497961 1102 21 1.2056486381775642 1102 22 1.2013133567089724 1102 23 1.2697064450376487 1102 24 0.985638025231971 1102 25 1.111963131405851 1103 1 1.0915752825711635 1103 2 3.6953550835503854 1103 3 2.147309193538838 1103 4 1.2595646188331244 1103 5 0.7952967877926653 1103 6 0.7997807346477614 1103 7 0.8903310615678137 1103 8 0.7170909627714792 1103 9 0.7653738619274222 1103 10 0.8808839140059561 1103 11 0.865126463865515 1103 12 0.7129719871569478 1103 13 0.8641918409076013 1103 14 0.8608661477541233 1103 15 0.7006297394979626 1103 16 0.6582547985511056 1103 17 0.724356799905145 1103 18 0.8594109301994379 1103 19 0.9569309558822923 1103 20 0.9435643149327007 1103 21 1.030352001870007 1103 22 1.1090476721776028 1103 23 1.1430599774589965 1103 24 0.9305289864424608 1103 25 1.0682750440296758 1104 1 1.3041871864753318 1104 2 4.066702047532829 1104 3 2.4310225038848507 1104 4 1.5966242020714958 1104 5 1.238578387396636 1104 6 0.9524111656478098 1104 7 0.9751733974522949 1104 8 0.9921288869525213 1104 9 1.1067576595409534 1104 10 1.1311075380137368 1104 11 1.01689670827124 1104 12 1.0599592510038676 1104 13 1.0785045126079567 1104 14 1.2982391722044966 1104 15 1.3052759724443348 1104 16 1.3271246211958214 1104 17 1.2374286953299816 1104 18 1.1172598265383726 1104 19 1.0854210220749962 1104 20 1.0643702926168572 1104 21 1.2212656376655318 1104 22 1.0974535332096806 1104 23 1.250854101232747 1104 24 1.123743805178357 1104 25 1.2282648492255959 1105 1 1.1117206022440413 1105 2 3.895626265868813 1105 3 2.31216128972952 1105 4 1.413456328315391 1105 5 1.0260729407735383 1105 6 0.8949207340397294 1105 7 0.6027586297434127 1105 8 0.6908264080029838 1105 9 0.8602499753862105 1105 10 0.8451109531936254 1105 11 0.8453204261067242 1105 12 0.8747328481304066 1105 13 1.0033010738751074 1105 14 0.9872893989017868 1105 15 0.9039605393641921 1105 16 0.9517798646745987 1105 17 1.0076000431483862 1105 18 1.077692023441159 1105 19 0.9334207865453479 1105 20 1.0824047977101898 1105 21 0.928592964992859 1105 22 0.9782307810457525 1105 23 0.9689828181849904 1105 24 1.1104493342368258 1105 25 1.1445599678736542 1106 1 0.41449402987728234 1106 2 3.203750792264369 1106 3 1.5099094584589956 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0.8149392234912227 1107 16 0.83365243094417 1107 17 0.9719555192221137 1107 18 1.0008695018801106 1107 19 0.8377606867443745 1107 20 0.6723831645508795 1107 21 0.7064429173999116 1107 22 0.7693029204209836 1107 23 0.7645165803356164 1107 24 0.9275781813336579 1107 25 1.0829510377054916 1108 1 1.0586401996657315 1108 2 3.8314489504781086 1108 3 2.1917858515298114 1108 4 1.285902330803232 1108 5 0.9793783801040874 1108 6 0.7560888896430313 1108 7 0.7854873417189836 1108 8 0.6568356808741811 1108 9 0.6680629347891269 1108 10 0.8195743705859115 1108 11 0.7697499183604819 1108 12 0.7113776519300217 1108 13 0.7223477774824758 1108 14 0.8064751955771214 1108 15 0.8974401277391806 1108 16 0.831552566246458 1108 17 0.8551099379804583 1108 18 0.7897331859425734 1108 19 0.6940868500984436 1108 20 0.6871905039184867 1108 21 0.7758913931281128 1108 22 0.804614361278702 1108 23 0.9847489608967379 1108 24 1.1032266722442898 1108 25 1.1097312898232161 1109 1 0.5915110048915864 1109 2 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16 -0.0979681712258369 2313 17 -0.2869320053629636 2313 18 -0.1620079587611727 2313 19 -0.19855043208626455 2313 20 -0.06640809797006142 2313 21 7.680940259433555E-4 2313 22 -0.06861979483586822 2313 23 0.0571992378031041 2313 24 -0.06361285589309773 2313 25 -0.14506094184132934 2314 1 0.1920623699028816 2314 2 2.4728566294406384 2314 3 1.1825502152403935 2314 4 0.5143832589984143 2314 5 0.10295849424446146 2314 6 -0.020711333299651358 2314 7 -0.2460249005035351 2314 8 -0.4051251157534139 2314 9 -0.2505060714673988 2314 10 -0.3143403930869937 2314 11 -0.3462489669260833 2314 12 -0.2835806248172119 2314 13 -0.32398151828553523 2314 14 -1.2316487888168908 2314 15 -0.6442124741746724 2314 16 -0.5731296226739175 2314 17 -0.416356145685846 2314 18 -0.29547566582409246 2314 19 -0.4717797610942256 2314 20 -0.34464555470601965 2314 21 -0.27456508987251027 2314 22 -0.24502767440782236 2314 23 -0.20749200560690895 2314 24 -0.2254940763407305 2314 25 -0.12729642886079517 2315 1 -4.243263506982778 2315 2 -14.19798538905987 2315 3 -7.0872224025234125 2315 4 -3.0936581615272303 2315 5 -1.750709749725047 2315 6 -1.2230899954103194 2315 7 -1.0124802403590536 2315 8 -1.024292963485152 2315 9 -0.9765433937119568 2315 10 -1.0257654547288837 2315 11 -1.0286171803614579 2315 12 -1.01966597213859 2315 13 -1.0592002609498437 2315 14 -1.259500156410816 2315 15 -1.4231788672107726 2315 16 -1.2889371270113514 2315 17 -1.2304879790224206 2315 18 -1.304999492157222 2315 19 -1.2644017961502314 2315 20 -1.2216291704065654 2315 21 -1.3661180771876076 2315 22 -1.288751497563993 2315 23 -1.3548421544828813 2315 24 -1.4794171832684526 2315 25 -1.5580430678996535 2316 1 -0.9928893305850686 2316 2 -1.1757483572866327 2316 3 -0.4429569204571848 2316 4 -0.535294927833128 2316 5 -0.807273438874212 2316 6 -0.9928753203316631 2316 7 -0.8864845077189045 2316 8 -1.0469119957628124 2316 9 -0.9870415039600644 2316 10 -1.1534705245659396 2316 11 -1.2731002646427356 2316 12 -1.2402615983708856 2316 13 -1.3150614373641218 2316 14 -1.4794192275328655 2316 15 -1.9253005808558328 2316 16 -1.6017495159780708 2316 17 -1.4918858615429968 2316 18 -1.3774875396836066 2316 19 -1.3310484576499206 2316 20 -1.5211875225944596 2316 21 -1.5120459518104354 2316 22 -1.7959248226988933 2316 23 -1.606990101897516 2316 24 -1.7152173500599943 2316 25 -1.7935983861751126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17808.0 3 0.0 4 161.0 5 790.0 6 1069.0 7 1021.0 8 883.0 9 871.0 10 835.0 11 819.0 12 829.0 13 893.0 14 833.0 15 843.0 16 882.0 17 959.0 18 1159.0 19 1381.0 20 1837.0 21 2234.0 22 2715.0 23 3479.0 24 3970.0 25 4694.0 26 5496.0 27 6421.0 28 7630.0 29 9509.0 30 12195.0 31 16353.0 32 22107.0 33 30904.0 34 45300.0 35 69004.0 36 112719.0 37 157085.0 38 389227.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.605493547541755 19.994331035441725 13.698464566297472 24.70171085071905 2 15.175964111354368 21.366529199124283 42.12774864371015 21.3297580458112 3 18.377285635592543 25.598690790071828 30.63850724397405 25.385516330361586 4 12.176294101602819 17.642887321307285 39.11703202964976 31.06378654744014 5 12.204438055264976 38.6306120135114 36.29713535148687 12.867814579736747 6 29.8193840703724 37.92848952628697 18.835997398685652 13.416129004654984 7 26.38528636293139 32.50477316119647 23.160073375654473 17.949867100217666 8 23.448441836958438 38.92428723466839 21.182674360770765 16.444596567602403 9 25.952733670975437 15.281603140392441 21.793211147537477 36.97245204109464 10 15.94348149296995 28.73566046111144 34.91525967601333 20.405598369905288 11 32.18474406764251 22.137306600065244 27.237770278581475 18.440179053710764 12 24.082938021103523 24.70802158484996 32.65665862672007 18.55238176732644 13 28.83288855136563 23.24307557371526 26.49000176486632 21.434034110052785 14 19.591513667017857 24.700213388382902 28.351133525507667 27.357139419091574 15 21.38515266093709 34.30183492616976 24.002609862928715 20.310402549964437 16 19.657295048212937 28.543022627725517 31.335041153473846 20.4646411705877 17 19.883775281764184 30.238963411568776 29.700731511916086 20.176529794750955 18 19.619751528213794 27.023633164512283 33.951963547488276 19.404651759785647 19 22.853438925933293 26.59046714457159 29.32879387836742 21.2273000511277 20 22.90758605346166 27.842376777304413 30.33779613718068 18.912241032053252 21 21.018364314892004 27.01176903469956 29.042657536385818 22.927209114022617 22 22.414687550474014 29.858367909704597 28.868622624980343 18.858321914841046 23 20.10762487244816 29.386553201684862 30.606237579071443 19.899584346795535 24 22.603341940259234 26.86879750477588 31.26175251843501 19.266108036529875 25 21.112338795160618 28.205405208826917 30.898062172629963 19.784193823382495 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 517.0 1 517.0 2 552.5 3 588.0 4 588.0 5 588.0 6 1275.5 7 1963.0 8 1963.0 9 1963.0 10 2496.0 11 3029.0 12 3029.0 13 3029.0 14 4287.0 15 5545.0 16 5545.0 17 5545.0 18 9683.5 19 13822.0 20 13822.0 21 13822.0 22 22291.5 23 30761.0 24 30761.0 25 30761.0 26 45359.0 27 59957.0 28 59957.0 29 59957.0 30 77465.5 31 94974.0 32 94974.0 33 94974.0 34 101869.5 35 108765.0 36 108765.0 37 108765.0 38 109878.0 39 110991.0 40 110991.0 41 110991.0 42 113191.0 43 115391.0 44 115391.0 45 115391.0 46 115308.0 47 115225.0 48 115225.0 49 115225.0 50 107064.5 51 98904.0 52 98904.0 53 98904.0 54 84632.5 55 70361.0 56 70361.0 57 70361.0 58 58913.5 59 47466.0 60 47466.0 61 47466.0 62 38646.0 63 29826.0 64 29826.0 65 29826.0 66 22817.0 67 15808.0 68 15808.0 69 15808.0 70 11364.5 71 6921.0 72 6921.0 73 6921.0 74 4836.0 75 2751.0 76 2751.0 77 2751.0 78 1853.0 79 955.0 80 955.0 81 955.0 82 616.5 83 278.0 84 278.0 85 278.0 86 179.5 87 81.0 88 81.0 89 81.0 90 56.5 91 32.0 92 32.0 93 32.0 94 17.5 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.462528679077777 3 0.0 4 0.0 5 1.0696159543915757E-4 6 3.208847863174727E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 2.1392319087831514E-4 18 0.0 19 1.0696159543915757E-4 20 2.1392319087831514E-4 21 4.278463817566303E-4 22 0.002994924672296412 23 1.0696159543915757E-4 24 9.626543589524182E-4 25 2.1392319087831514E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 934915.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.36227727413737 #Duplication Level Percentage of deduplicated Percentage of total 1 83.71282422416404 56.39086476789262 2 11.38914449852661 15.343974192499315 3 2.661474486283718 5.3784894690925835 4 0.8708754560428248 2.346566157647904 5 0.4085434629250646 1.3760209014047236 6 0.21756360330430347 0.8793347862326952 7 0.12812987540498186 0.6041784135891745 8 0.09797320304947488 0.5279758455403271 9 0.06871142864741916 0.41657024776046003 >10 0.371184021078766 4.671166418912484 >50 0.03428429884942604 1.6274714159917794 >100 0.0320152490244287 4.19658655562321 >500 0.00452740879044461 2.0344115374607115 >1k 0.00258709073739692 3.6354295902037785 >5k 1.616931710873075E-4 0.570959700148252 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5242 0.5606926832920639 No Hit GGTATCAACGCAGAGTACTTTTTTT 3795 0.40591925469160295 No Hit GTCCTACAGTGGACATTTCTAAATT 3645 0.38987501537572933 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3457 0.3697662354331677 No Hit CTGTAGGACGTGGAATATGGCAAGA 3312 0.3542568040944899 No Hit TATCAACGCAGAGTACTTTTTTTTT 3251 0.34773214677270126 No Hit CTTTAGGACGTGAAATATGGCGAGG 3080 0.3294417139526053 No Hit GTCCTACAGTGTGCATTTCTCATTT 1549 0.16568351133525508 No Hit CTGAAGGACCTGGAATATGGCGAGA 1478 0.1580892380590749 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1470 0.15723354529556163 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1265 0.13530641823053433 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1263 0.135092495039656 No Hit ATTTAGAAATGTCCACTGTAGGACG 1262 0.13498553344421685 No Hit GAATATGGCAAGAAAACTGAAAATC 1234 0.13199060877192043 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1194 0.12771214495435415 No Hit CTGTAGGACCTGGAATATGGCGAGA 1090 0.11658813902868176 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1032 0.11038436649321061 No Hit GGAATATGGCGAGAAAACTGAAAAT 947 0.10129263088088222 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 1.0696159543915757E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATCGC 35 0.0022537534 16.19288 19 CGGTAGG 65 1.995355E-6 15.370603 1 GGTATCA 860 0.0 14.870166 1 TAGGACC 610 0.0 14.245434 4 ATTGCGA 40 0.005482902 14.168013 17 GTATAAG 85 1.4577381E-7 14.104789 1 GTCAACG 50 0.0010246512 13.987249 2 GTTATAG 50 0.0010246512 13.987249 1 CCGTAGG 60 2.6713804E-4 13.32119 1 TCCAACG 100 1.089029E-8 13.224185 18 CGTGCGC 55 0.0032022796 12.021344 10 GCGTGCG 55 0.0032022796 12.021344 9 CGTAGGA 75 1.296499E-4 11.989071 2 GTGCTAG 70 9.781264E-4 11.418162 1 CTCTTAC 80 2.362834E-4 11.239753 1 ATAGGAC 160 5.0931703E-11 11.216343 3 GTGCGCC 60 0.00613034 11.019566 11 CTATAGG 155 1.5879777E-9 10.957752 1 TATAGGA 185 2.1827873E-11 10.800963 2 GGCGTGC 70 0.0015639167 10.794677 8 >>END_MODULE