FastQCFastQC Report
Thu 26 May 2016
SRR1511799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511799_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934915
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66050.7064813378756357No Hit
GGTATCAACGCAGAGTACTTTTTTT49550.5299947054010258No Hit
TATCAACGCAGAGTACTTTTTTTTT46680.49929672750998755No Hit
CTGTAGGACGTGGAATATGGCAAGA35440.3790718942363744No Hit
GTCCTACAGTGGACATTTCTAAATT34620.3703010434103635No Hit
GTCCTAAAGTGTGTATTTCTCATTT33900.36259980853874413No Hit
CTTTAGGACGTGAAATATGGCGAGG31850.3406726814737168No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21500.2299674301941888No Hit
CTGAAGGACCTGGAATATGGCGAGA16730.1789467491697106No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16430.17573790130653588No Hit
GTCCTACAGTGTGCATTTCTCATTT16330.1746682853521443No Hit
TTTCTAAATTTTCCACCTTTTTCAG14610.1562708909366092No Hit
ATTTAGAAATGTCCACTGTAGGACG14020.1499601568056989No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13640.14589561617901092No Hit
GAATATGGCAAGAAAACTGAAAATC13440.14375638427022777No Hit
GGAATATGGCGAGAAAACTGAAAAT11930.127605183358915No Hit
GTCCTTCAGTGTGCATTTCTCATTT11680.12493114347293605No Hit
CTGTAGGACCTGGAATATGGCGAGA11600.12407545070942279No Hit
ATTCCAGGTCCTTCAGTGTGCATTT10250.10963563532513651No Hit
TTCCAGGTCCTTCAGTGTGCATTTC9510.10172047726263884No Hit
GCCATATTCCACGTCCTACAGTGGA9480.10139959247632137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA350.002172636316.28200117
GGTATCA9100.013.7218761
TAGGACC4650.013.0743854
GTGGTAT2550.012.3355151
ACACGGT550.003072115112.0881536
GTATTAA959.969644E-712.0404081
GTAAGAC1353.7107384E-1011.9621543
TGGTATC2500.011.7792042
ATCCTAG657.795155E-411.731681
TGTTAGA907.4571617E-611.6103272
CCACCTT4300.011.48575313
TCCAACG752.0754925E-411.3980118
GTATCAA21100.011.2938271
CACCTTT4400.011.22471314
GTATAGG600.005727533711.1206541
ATAGGAC1201.2737837E-711.0825833
CCTAGGG600.005875646611.0825833
CTAGCAC600.005875646611.0825833
ATGTCCA4800.010.8829359
TAAGACG700.001491279210.856414
Produced by FastQC (version 0.11.5)