##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511799_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 934915 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.218828449645155 31.0 30.0 34.0 26.0 34.0 2 30.430818844493885 31.0 30.0 34.0 27.0 34.0 3 30.524742891064964 31.0 30.0 34.0 27.0 34.0 4 34.151412695271766 37.0 35.0 37.0 30.0 37.0 5 33.725803950091716 37.0 35.0 37.0 28.0 37.0 6 33.66301535433703 37.0 35.0 37.0 29.0 37.0 7 33.46619532256943 37.0 35.0 37.0 28.0 37.0 8 33.352032002909354 36.0 35.0 37.0 27.0 37.0 9 34.74791184225304 38.0 35.0 39.0 28.0 39.0 10 34.51190856922822 38.0 35.0 39.0 27.0 39.0 11 34.488721434568916 38.0 35.0 39.0 27.0 39.0 12 34.29446741147591 38.0 34.0 39.0 27.0 39.0 13 34.28038270858848 38.0 34.0 39.0 27.0 39.0 14 35.129198911130956 39.0 34.0 40.0 26.0 41.0 15 35.05407443457427 38.0 34.0 40.0 26.0 41.0 16 34.93174994518218 38.0 34.0 40.0 25.0 41.0 17 34.90672948877705 38.0 34.0 40.0 25.0 41.0 18 34.814722193996246 38.0 34.0 40.0 25.0 41.0 19 34.85131910387575 39.0 34.0 40.0 25.0 41.0 20 34.71526716332501 39.0 34.0 40.0 25.0 41.0 21 34.56127134552339 38.0 34.0 40.0 24.0 41.0 22 34.63789328441623 39.0 34.0 40.0 24.0 41.0 23 34.4972280902542 39.0 34.0 40.0 23.0 41.0 24 34.35134530946664 39.0 34.0 40.0 22.0 41.0 25 33.882874913762215 38.0 34.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5991137529686306 1101 2 0.4084465780321871 1101 3 0.6239675912823799 1101 4 0.40317015087376973 1101 5 0.5719087874883542 1101 6 0.6504707088045052 1101 7 0.6425708229187208 1101 8 0.7285930046866511 1101 9 0.6247919543748921 1101 10 0.7007993747507939 1101 11 0.3789563067122117 1101 12 0.4953944912911723 1101 13 0.5656437953113311 1101 14 0.6812270945627361 1101 15 0.2621069894096877 1101 16 0.6807500031705302 1101 17 0.558132425760391 1101 18 0.49666570918884645 1101 19 0.35281053283470243 1101 20 0.6696308690197839 1101 21 0.5522125079085782 1101 22 0.4711252318590198 1101 23 0.6075400234435122 1101 24 0.2239870271041795 1101 25 0.5051207207674651 1102 1 0.9823103626580334 1102 2 1.1076246020214704 1102 3 1.111319292429549 1102 4 0.951494605340983 1102 5 1.0036422907729872 1102 6 1.1594231115455145 1102 7 1.2007382903174886 1102 8 0.9262076945799933 1102 9 1.129193257334876 1102 10 1.2443303309447558 1102 11 1.1629220549881865 1102 12 1.2928582949320528 1102 13 1.1588146944190854 1102 14 1.5693751700821252 1102 15 1.540388629221873 1102 16 1.7929450985502484 1102 17 1.7519041462994451 1102 18 2.0465834571162063 1102 19 1.900696491153056 1102 20 1.9250044895272396 1102 21 1.9765297067624914 1102 22 1.4025645886587057 1102 23 1.752262817617229 1102 24 1.7822007953385395 1102 25 1.9739275754700003 1103 1 0.3809641599656288 1103 2 0.44403920342210057 1103 3 0.5657262012433684 1103 4 0.6736815497148712 1103 5 0.6898155916910156 1103 6 0.7898070151704673 1103 7 0.759711765407296 1103 8 0.6477959234707384 1103 9 0.7227198729595301 1103 10 0.9604756918069057 1103 11 0.7646674013360197 1103 12 0.9040016600759202 1103 13 0.8970943852641824 1103 14 1.031837523578261 1103 15 1.0815457829647528 1103 16 0.6891133194581229 1103 17 0.5164275324890752 1103 18 0.8138827060004132 1103 19 0.8515473928548616 1103 20 0.7064733381792223 1103 21 0.7420690949841244 1103 22 1.1495710112429833 1103 23 1.2199049671872473 1103 24 1.3214801596115535 1103 25 1.373806018084025 1104 1 0.7503444580166061 1104 2 0.7063189184577219 1104 3 0.6522668542499765 1104 4 0.835933327066229 1104 5 0.817284952138607 1104 6 0.6726280350919964 1104 7 0.7978111696484333 1104 8 0.9735983354241498 1104 9 0.9755081273689115 1104 10 0.8986856214480525 1104 11 0.9496001670932444 1104 12 0.9730285709277524 1104 13 0.9917087839545431 1104 14 0.979192143466463 1104 15 1.1121510259368321 1104 16 1.3018504528066472 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1.4123919064330366 1107 17 1.1218095705134061 1107 18 1.2312103426072056 1107 19 1.448348326356104 1107 20 1.28690318572896 1107 21 1.06952922970315 1107 22 1.2208357157483007 1107 23 1.2169493601173187 1107 24 1.2595060493849317 1107 25 1.0189518956604289 1108 1 0.2906130061611094 1108 2 0.2823300760588374 1108 3 0.30399518854045837 1108 4 0.2585343060910361 1108 5 0.5255943567930785 1108 6 0.5007358007459075 1108 7 0.5007787816118565 1108 8 0.5608605299346792 1108 9 0.4543961958459519 1108 10 0.12854451061041772 1108 11 0.5174619003500069 1108 12 0.46505234463993617 1108 13 0.49803051301808665 1108 14 0.6995980650168576 1108 15 0.8110006262139464 1108 16 0.5645645575094278 1108 17 0.6637488139816057 1108 18 0.47118770291763923 1108 19 0.8547195904724205 1108 20 0.8990466775996566 1108 21 0.9941964441282494 1108 22 0.917199299338769 1108 23 1.1296079384162567 1108 24 0.993493046076054 1108 25 0.8520053958814628 1109 1 -0.022297932272842047 1109 2 -0.14402121759670194 1109 3 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-0.8139191198269593 2314 4 -0.844994703933537 2314 5 -0.8554394221639541 2314 6 -0.827290214467574 2314 7 -1.0723507370988443 2314 8 -1.0600603646620996 2314 9 -1.1789572860635076 2314 10 -1.1811436836323281 2314 11 -1.3660505459749714 2314 12 -1.2288214415391892 2314 13 -1.1719515102652949 2314 14 -1.2595559302887835 2314 15 -1.5282451366974996 2314 16 -1.6055038168144833 2314 17 -1.2064655721017417 2314 18 -1.14190272232198 2314 19 -1.1747221893010291 2314 20 -1.0661636656849751 2314 21 -0.9186380680276258 2314 22 -1.0505505495469123 2314 23 -0.9851486694008642 2314 24 -1.2044412015316297 2314 25 -0.9965270289342527 2315 1 -0.792557306012931 2315 2 -0.7105829316520307 2315 3 -0.9115401047205047 2315 4 -0.7499470583271872 2315 5 -1.0335134418505305 2315 6 -1.1861307292060275 2315 7 -1.0146518107355291 2315 8 -1.0995985402739734 2315 9 -1.244017939564607 2315 10 -1.2261091694692539 2315 11 -1.3880986628307 2315 12 -1.415659464127387 2315 13 -1.2354210352565147 2315 14 -1.4413240508862657 2315 15 -1.355355742632419 2315 16 -1.6580587120718064 2315 17 -1.6097429614861056 2315 18 -1.7748938850724514 2315 19 -1.5433406873392528 2315 20 -1.494135757838059 2315 21 -1.936426408223788 2315 22 -1.8558998477030357 2315 23 -1.6947602151870669 2315 24 -1.8756062111772351 2315 25 -1.7597136278021992 2316 1 -1.760067012507676 2316 2 -1.3057489223772443 2316 3 -1.1234196760699149 2316 4 -1.0473533209262982 2316 5 -1.0651191510551783 2316 6 -1.0646315567203786 2316 7 -1.1569253672798894 2316 8 -1.0999428009695293 2316 9 -1.2914872552590282 2316 10 -1.3948180814029456 2316 11 -1.4597343429664917 2316 12 -1.4169453790784274 2316 13 -1.3140247727862686 2316 14 -1.4994967445697256 2316 15 -1.5026799901834735 2316 16 -1.5197561198071767 2316 17 -1.5010154926122397 2316 18 -1.55164266497205 2316 19 -1.587972153503216 2316 20 -1.3975761556340558 2316 21 -1.7986107515419079 2316 22 -1.529936516069526 2316 23 -1.4394706588047157 2316 24 -1.1877190329551297 2316 25 -1.3623990453241461 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21125.0 3 0.0 4 360.0 5 3124.0 6 3944.0 7 3123.0 8 2529.0 9 2254.0 10 2097.0 11 2040.0 12 1890.0 13 1773.0 14 1775.0 15 1859.0 16 1849.0 17 1931.0 18 2117.0 19 2450.0 20 2968.0 21 3537.0 22 4658.0 23 5885.0 24 7138.0 25 8366.0 26 9623.0 27 10862.0 28 12670.0 29 15238.0 30 19667.0 31 25112.0 32 32075.0 33 41481.0 34 53912.0 35 73329.0 36 108172.0 37 184446.0 38 259536.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.32827436934732 21.545474846899772 14.719201731557114 23.4070490521958 2 14.650315804110534 21.996652102062754 42.01911403710498 21.333918056721735 3 18.94225678270217 26.534069942187255 31.326484225838712 23.19718904927186 4 13.354048228983384 18.98921292309996 38.262622805281765 29.394116042634895 5 12.334878585543244 39.11966628543551 36.12060028394411 12.424854845077144 6 29.962402999203135 38.72715701427403 18.19823192482739 13.11220806169545 7 26.289127888631587 32.986421225459 23.033003000272753 17.691447885636663 8 23.827941577576574 39.18805452902136 20.836760561120528 16.147243332281544 9 25.99070503735634 15.155281496178796 21.731280383778202 37.12273308268666 10 15.98669397752737 29.40983939716445 34.58346480696106 20.020001818347122 11 32.379948979318975 22.289940796756923 26.977746640068883 18.352363583855215 12 24.15695544514742 25.201863270992554 32.470117604274186 18.171063679585846 13 29.322451773690656 23.229063604712728 26.27233491814764 21.176149703448978 14 19.87175304706845 24.764176422455517 28.454886273083652 26.909184257392383 15 21.81727750651129 34.57180599305819 23.771786739971013 19.839129760459507 16 19.8016932020558 28.99803725472369 30.92559216613275 20.27467737708776 17 20.017434740056583 30.711668975254437 29.410374205141643 19.860522079547337 18 19.794868832855215 27.18645779183968 33.92023223526442 19.09844114004069 19 23.0424156206714 26.761791178877225 29.521079456421173 20.674713744030207 20 23.1563297198141 28.046613863292386 30.252696769225008 18.544359647668504 21 21.32012001091008 27.05529379676227 29.355930753063113 22.26865543926453 22 22.628153361535542 29.654995373910996 29.141686677398482 18.575164587154983 23 20.33008348352524 29.426525406052956 30.50790713594284 19.735483974478964 24 22.780677734040733 27.171080780421015 31.13886274174226 18.909378743795997 25 21.328271905223367 28.544871506897962 30.873641853689215 19.253214734189456 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 482.0 1 482.0 2 564.0 3 646.0 4 646.0 5 646.0 6 1468.5 7 2291.0 8 2291.0 9 2291.0 10 2827.0 11 3363.0 12 3363.0 13 3363.0 14 4492.0 15 5621.0 16 5621.0 17 5621.0 18 9897.5 19 14174.0 20 14174.0 21 14174.0 22 23125.5 23 32077.0 24 32077.0 25 32077.0 26 47300.5 27 62524.0 28 62524.0 29 62524.0 30 78980.5 31 95437.0 32 95437.0 33 95437.0 34 103032.5 35 110628.0 36 110628.0 37 110628.0 38 111095.0 39 111562.0 40 111562.0 41 111562.0 42 113261.0 43 114960.0 44 114960.0 45 114960.0 46 114594.0 47 114228.0 48 114228.0 49 114228.0 50 105991.0 51 97754.0 52 97754.0 53 97754.0 54 83331.0 55 68908.0 56 68908.0 57 68908.0 58 57434.5 59 45961.0 60 45961.0 61 45961.0 62 37302.5 63 28644.0 64 28644.0 65 28644.0 66 21793.0 67 14942.0 68 14942.0 69 14942.0 70 10874.0 71 6806.0 72 6806.0 73 6806.0 74 4715.0 75 2624.0 76 2624.0 77 2624.0 78 1784.5 79 945.0 80 945.0 81 945.0 82 599.5 83 254.0 84 254.0 85 254.0 86 161.5 87 69.0 88 69.0 89 69.0 90 41.0 91 13.0 92 13.0 93 13.0 94 7.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37425862244161234 2 0.0 3 0.0 4 0.0 5 0.023959397378371296 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0696159543915757E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.003743655840370515 25 1.0696159543915757E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 934915.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.22369680952072 #Duplication Level Percentage of deduplicated Percentage of total 1 83.99432994238356 55.624150398232565 2 11.042168289534434 14.625064098516646 3 2.6200905444911347 5.205362454956188 4 0.9159840553040208 2.426394014432349 5 0.4125226496629735 1.3659387439170443 6 0.22398652757694032 0.8899929535003589 7 0.1371062189322073 0.6355776471286386 8 0.10654483448841703 0.5644634252625198 9 0.07859263360461072 0.46842252653641403 >10 0.3900513661671387 4.775354395853647 >50 0.03778758301242716 1.750256073811985 >100 0.03295766854957898 4.400610806115973 >500 0.004823073240337243 2.261790187890711 >1k 0.0028938439442023458 4.303406109703565 >5k 1.6076910801124142E-4 0.7032161641413173 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6605 0.7064813378756357 No Hit GGTATCAACGCAGAGTACTTTTTTT 4955 0.5299947054010258 No Hit TATCAACGCAGAGTACTTTTTTTTT 4668 0.49929672750998755 No Hit CTGTAGGACGTGGAATATGGCAAGA 3544 0.3790718942363744 No Hit GTCCTACAGTGGACATTTCTAAATT 3462 0.3703010434103635 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3390 0.36259980853874413 No Hit CTTTAGGACGTGAAATATGGCGAGG 3185 0.3406726814737168 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2150 0.2299674301941888 No Hit CTGAAGGACCTGGAATATGGCGAGA 1673 0.1789467491697106 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1643 0.17573790130653588 No Hit GTCCTACAGTGTGCATTTCTCATTT 1633 0.1746682853521443 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1461 0.1562708909366092 No Hit ATTTAGAAATGTCCACTGTAGGACG 1402 0.1499601568056989 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1364 0.14589561617901092 No Hit GAATATGGCAAGAAAACTGAAAATC 1344 0.14375638427022777 No Hit GGAATATGGCGAGAAAACTGAAAAT 1193 0.127605183358915 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1168 0.12493114347293605 No Hit CTGTAGGACCTGGAATATGGCGAGA 1160 0.12407545070942279 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1025 0.10963563532513651 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 951 0.10172047726263884 No Hit GCCATATTCCACGTCCTACAGTGGA 948 0.10139959247632137 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.0696159543915757E-4 13 0.0 0.0 0.0 0.0 1.0696159543915757E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGCGA 35 0.0021726363 16.282001 17 GGTATCA 910 0.0 13.721876 1 TAGGACC 465 0.0 13.074385 4 GTGGTAT 255 0.0 12.335515 1 ACACGGT 55 0.0030721151 12.088153 6 GTATTAA 95 9.969644E-7 12.040408 1 GTAAGAC 135 3.7107384E-10 11.962154 3 TGGTATC 250 0.0 11.779204 2 ATCCTAG 65 7.795155E-4 11.73168 1 TGTTAGA 90 7.4571617E-6 11.610327 2 CCACCTT 430 0.0 11.485753 13 TCCAACG 75 2.0754925E-4 11.39801 18 GTATCAA 2110 0.0 11.293827 1 CACCTTT 440 0.0 11.224713 14 GTATAGG 60 0.0057275337 11.120654 1 ATAGGAC 120 1.2737837E-7 11.082583 3 CCTAGGG 60 0.0058756466 11.082583 3 CTAGCAC 60 0.0058756466 11.082583 3 ATGTCCA 480 0.0 10.882935 9 TAAGACG 70 0.0014912792 10.85641 4 >>END_MODULE