FastQCFastQC Report
Thu 26 May 2016
SRR1511798_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511798_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220168
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67510.30407608793568774No Hit
GTCCTACAGTGGACATTTCTAAATT56150.2529087888844448No Hit
CTGTAGGACGTGGAATATGGCAAGA51640.23259501082800943No Hit
GTCCTAAAGTGTGTATTTCTCATTT48340.21773127078671523No Hit
GGTATCAACGCAGAGTACTTTTTTT43050.1939042450841558No Hit
CTTTAGGACGTGAAATATGGCGAGG40370.1818330865051654No Hit
TATCAACGCAGAGTACTTTTTTTTT39910.17976117122668195No Hit
GTCCTACAGTGTGCATTTCTCATTT27340.12314383416029777No Hit
CTGAAGGACCTGGAATATGGCGAGA22740.10242468137546348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGA801.1348257E-613.7377972
TAGGACC8700.013.6793124
GGTATCA12750.013.243141
CGGTAGG1152.5156623E-913.0319021
TCCAACG1251.1277734E-1012.84668318
GTCCTAG3000.011.989351
ACCGAGC658.426086E-411.6246498
GTCGAGG1657.2759576E-1211.44980519
GGCGAGG12400.011.35054319
TGGACCG752.1889774E-411.3342875
TATGTCG2100.011.24509116
GAACAGT3550.011.1746496
GGACCTG17900.011.0810066
ATGTCGA2050.011.05883717
TAGAGCG600.006135334711.0194455
TGTCGAG2250.010.91548218
AGGACCT18800.010.8017375
CGTAAGA650.00732545210.7596742
CGTGCGC1159.5053656E-710.67698610
GTATCAA26650.010.6097131
Produced by FastQC (version 0.11.5)