##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511798_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2220168 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.708381527884377 31.0 30.0 34.0 25.0 34.0 2 26.91499066737292 31.0 26.0 34.0 10.0 34.0 3 28.69307998313641 31.0 28.0 34.0 16.0 34.0 4 33.104749730651015 35.0 33.0 37.0 28.0 37.0 5 33.4858380086552 35.0 35.0 37.0 28.0 37.0 6 33.70090596747634 37.0 35.0 37.0 29.0 37.0 7 33.69846426036228 37.0 35.0 37.0 29.0 37.0 8 33.719698689468544 37.0 35.0 37.0 29.0 37.0 9 35.295353324613274 39.0 35.0 39.0 29.0 39.0 10 35.16618156824168 39.0 35.0 39.0 29.0 39.0 11 35.170101992281666 39.0 35.0 39.0 29.0 39.0 12 35.05835774590031 39.0 35.0 39.0 28.0 39.0 13 35.05446569809131 39.0 35.0 39.0 28.0 39.0 14 36.16221745381431 40.0 36.0 41.0 28.0 41.0 15 36.11001239545836 40.0 36.0 41.0 27.0 41.0 16 36.0610787111606 40.0 36.0 41.0 27.0 41.0 17 36.029451374850915 40.0 36.0 41.0 27.0 41.0 18 36.00337181690755 40.0 36.0 41.0 27.0 41.0 19 36.02959685933677 40.0 36.0 41.0 27.0 41.0 20 35.97565364422873 40.0 36.0 41.0 27.0 41.0 21 35.86329367867657 40.0 36.0 41.0 27.0 41.0 22 35.77799968290688 40.0 36.0 41.0 27.0 41.0 23 35.659574410585144 40.0 36.0 41.0 27.0 41.0 24 35.55505529311295 40.0 36.0 41.0 27.0 41.0 25 35.45353414696545 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8676070419948019 1101 2 3.505971845376834 1101 3 2.0525285342650577 1101 4 1.196187168350761 1101 5 0.4637423450014424 1101 6 0.4806005755583129 1101 7 0.4320207851708773 1101 8 0.30165739534354685 1101 9 0.314845484732011 1101 10 0.4191999456671809 1101 11 0.31901473690128057 1101 12 0.41842670836091855 1101 13 0.3086307786113167 1101 14 0.3162888413150853 1101 15 0.4355640513087735 1101 16 0.4224507596598741 1101 17 0.26928980975990413 1101 18 0.432987189664658 1101 19 0.3810539686294163 1101 20 0.33638870459420644 1101 21 0.3219122781449002 1101 22 0.40706004723762845 1101 23 0.4140847054721064 1101 24 0.256952291121479 1101 25 0.49626428455609073 1102 1 1.1633668448044645 1102 2 3.8769754897688564 1102 3 2.122912178354639 1102 4 1.2911651659010062 1102 5 0.9410200900149803 1102 6 0.803962489393399 1102 7 0.7045215034639156 1102 8 0.7030289569882058 1102 9 0.869789609095001 1102 10 0.8503012779117327 1102 11 0.8135042951888707 1102 12 0.7404155483554788 1102 13 0.8443926984951844 1102 14 0.8991722963801863 1102 15 1.0237538016452419 1102 16 1.0077764110382361 1102 17 0.9465841889278934 1102 18 0.8638873998598839 1102 19 1.0304634561462365 1102 20 0.9535022251373917 1102 21 1.0345542355312176 1102 22 1.1151427341072306 1102 23 1.2114549968722486 1102 24 0.9923467474114602 1102 25 0.8749075424308543 1103 1 1.2419738099785924 1103 2 3.795538537961832 1103 3 2.2005457817380183 1103 4 1.4366150564778124 1103 5 0.9701394609977712 1103 6 0.974168116379694 1103 7 0.9376167890350686 1103 8 0.791549245205708 1103 9 0.7726575061264143 1103 10 1.0662275840357438 1103 11 0.9840791793036772 1103 12 0.8471512907223158 1103 13 1.0742230125360592 1103 14 1.2463564646038847 1103 15 0.9752073207887619 1103 16 0.9887781317764563 1103 17 0.9706752625060702 1103 18 1.161657579146528 1103 19 1.0501357527532562 1103 20 1.0274068238656682 1103 21 1.1278428641348626 1103 22 1.173795044056746 1103 23 1.2252696445640225 1103 24 1.286499191524392 1103 25 1.140546935766011 1104 1 1.129738742541214 1104 2 3.6956149514201364 1104 3 2.129359709485115 1104 4 1.2712833610524186 1104 5 1.0610111750522222 1104 6 0.8785836034761587 1104 7 0.6638246984715366 1104 8 0.7937789641776973 1104 9 0.981627816899902 1104 10 0.9665452027959844 1104 11 0.9336348562563686 1104 12 1.039200525205331 1104 13 1.0057233285817446 1104 14 1.2286304967080284 1104 15 1.0388430950625462 1104 16 0.9839935910342135 1104 17 0.9745843614755572 1104 18 0.8382425029571152 1104 19 0.9796668610868551 1104 20 0.9627349631812763 1104 21 0.9667401268837068 1104 22 0.9379837314327446 1104 23 1.0325130016460662 1104 24 0.8545300505489521 1104 25 0.9717973707459251 1105 1 1.183593395665973 1105 2 3.8399276779803415 1105 3 2.2143912238053503 1105 4 1.3456413724389407 1105 5 0.9959305658842226 1105 6 0.8207556422730633 1105 7 0.678188479319104 1105 8 0.712511206756318 1105 9 0.8754969481061536 1105 10 0.8600224460821835 1105 11 0.8046629088999779 1105 12 0.6531990882829604 1105 13 0.8349989890761762 1105 14 0.9218203555195146 1105 15 1.1085501588423057 1105 16 1.0080293834642475 1105 17 0.9949679762061905 1105 18 1.004764933097647 1105 19 0.9156448935917751 1105 20 1.0484849386882829 1105 21 0.9750242020826434 1105 22 0.9722793673253207 1105 23 1.0478619451815447 1105 24 0.9096964396283411 1105 25 1.0797407211699266 1106 1 1.009794583909347 1106 2 3.775275233413158 1106 3 2.1968667436138816 1106 4 1.144576824512228 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0.8103024827049055 1107 18 0.5976842625410157 1107 19 0.5718997174626068 1107 20 0.6543708329181612 1107 21 0.6092667385896959 1107 22 0.6862122499161671 1107 23 0.786840365113008 1107 24 0.7700280132135973 1107 25 0.8052123027821025 1108 1 1.006316263641434 1108 2 3.7523857289524614 1108 3 2.1797910672288907 1108 4 1.452494002740636 1108 5 0.8457646812687756 1108 6 0.8145661348905335 1108 7 0.7533422716441933 1108 8 0.6224012043129292 1108 9 0.6532759725118069 1108 10 0.6869011558389602 1108 11 0.5834774655243109 1108 12 0.5439745406842107 1108 13 0.7558381192291108 1108 14 0.7706621551182664 1108 15 0.7990573369255927 1108 16 0.9162152407203692 1108 17 0.8339364830618123 1108 18 0.8274653617714236 1108 19 0.7674840021364986 1108 20 0.7471500495633592 1108 21 0.7682177801207146 1108 22 0.7358318744601888 1108 23 0.8899179337325194 1108 24 0.9421748355673287 1108 25 0.8398616773168825 1109 1 0.7371828692120417 1109 2 2.9095599542062516 1109 3 1.5054624063089435 1109 4 0.888643367228859 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0.17128614190329472 2314 2 2.2232766386537044 2314 3 0.9667016826197141 2314 4 0.3314982679665519 2314 5 0.05180825445593484 2314 6 -0.20918862021415663 2314 7 -0.28239065109177375 2314 8 -0.4302646212213972 2314 9 -0.43916370348157585 2314 10 -0.42094066833092825 2314 11 -0.38682464023389684 2314 12 -0.47189993913217165 2314 13 -0.45401021586119583 2314 14 -1.42440860816356 2314 15 -1.0100070903985738 2314 16 -0.8755241434310577 2314 17 -0.6922235013949845 2314 18 -0.621791199077812 2314 19 -0.5515460124539047 2314 20 -0.4781260338748865 2314 21 -0.4775808248564317 2314 22 -0.31132194595002716 2314 23 -0.3449936473442392 2314 24 -0.4211663631785001 2314 25 -0.3253743749128901 2315 1 -4.4140651997738445 2315 2 -13.768634420171317 2315 3 -7.139831865865371 2315 4 -3.2046755853315787 2315 5 -2.0886544928098303 2315 6 -1.3879649700834165 2315 7 -1.0595739980233958 2315 8 -1.151125253206132 2315 9 -1.1472400214469687 2315 10 -1.13827832967673 2315 11 -1.2011213761543331 2315 12 -1.1293694352038486 2315 13 -1.1705935107436929 2315 14 -1.2422231271025481 2315 15 -1.1960429385845899 2315 16 -1.231617392636558 2315 17 -1.2269353103499157 2315 18 -1.378676972576507 2315 19 -1.3544148529832825 2315 20 -1.2638495008538086 2315 21 -1.4643264547906156 2315 22 -1.343978016229606 2315 23 -1.4401467095244982 2315 24 -1.603191316208921 2315 25 -1.4942074975231634 2316 1 -1.1283247553568074 2316 2 -1.1422124017159483 2316 3 -0.6047241649385882 2316 4 -0.7627332366191553 2316 5 -0.9924357405645807 2316 6 -0.909147223770141 2316 7 -1.0311163213708099 2316 8 -1.2282757447840496 2316 9 -1.0449752637104197 2316 10 -1.3302747099272807 2316 11 -1.1600789325267584 2316 12 -1.0204974130106308 2316 13 -0.945760644908006 2316 14 -1.157576723159572 2316 15 -1.684765254875181 2316 16 -1.509550679758263 2316 17 -1.5292342759404747 2316 18 -1.44667977203558 2316 19 -1.4888309211337045 2316 20 -1.5022234118965159 2316 21 -1.4560458852298481 2316 22 -1.5600250485382858 2316 23 -1.3873979659213873 2316 24 -1.3469916517135019 2316 25 -1.5428105750083176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72059.0 3 2.0 4 663.0 5 3331.0 6 3936.0 7 3562.0 8 3406.0 9 3197.0 10 3202.0 11 3036.0 12 2968.0 13 2993.0 14 2974.0 15 3078.0 16 3268.0 17 3481.0 18 4071.0 19 4840.0 20 6440.0 21 8291.0 22 10239.0 23 12251.0 24 14341.0 25 16321.0 26 18443.0 27 21133.0 28 24414.0 29 29660.0 30 37196.0 31 48031.0 32 62481.0 33 83272.0 34 114698.0 35 165019.0 36 256597.0 37 348055.0 38 819219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95607359443069 19.245210272375786 12.608054885936559 25.190661247256962 2 15.80961719235004 21.25721870686702 40.88871863992678 22.044445460856156 3 18.691108060290933 25.146250193679037 29.894044054323814 26.268597691706212 4 11.811178253177237 16.99736236176722 39.44782556995687 31.743633815098676 5 12.386280616064262 38.445822324357096 36.457495367446825 12.71040169213182 6 30.57908752029144 38.09705479094186 18.627812199907392 12.696045488859301 7 27.290817631818854 31.860877194878945 22.957767159962668 17.89053801333953 8 24.072817912878666 38.216252103444425 21.061469222148954 16.649460761527955 9 26.246031831825338 15.18939107310798 20.91256157191708 37.6520155231496 10 14.517414898332017 29.26877605658671 35.50506087827588 20.708748166805393 11 33.555658851041905 22.40911498589296 25.82606361320405 18.20916254986109 12 23.261527956442933 24.838345566641802 32.615144439519895 19.284982037395366 13 28.741473618212677 22.562436716500734 26.1776135859989 22.518476079287694 14 20.02483595835991 23.103657020549797 28.462035305436345 28.40947171565395 15 22.01477545843378 32.47929886386976 23.7770745276934 21.728851150003063 16 20.946207674374193 28.685486864057136 29.281477798076544 21.086827663492134 17 20.821564260153007 29.440376910621108 28.710022498429165 21.028036330796724 18 20.48592719109545 27.643538687162412 31.607067573264725 20.263466548477414 19 22.798416152327555 27.11697294297761 28.56141643654092 21.523194468153914 20 22.91164355641836 27.751485819683765 29.395965596996604 19.940905026901277 21 21.9925932999182 27.164131104536988 28.165053563900127 22.67822203164468 22 22.43998371218127 29.008758306066852 28.262878907995447 20.28837907375643 23 21.05365331613338 28.649228591980492 29.101357964580043 21.19576012730608 24 22.33844221863783 27.080710538489157 29.962267788348285 20.618579454524735 25 21.730658617957623 28.040054969283595 29.37289344093572 20.856392971823063 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 239.0 1 239.0 2 423.0 3 607.0 4 607.0 5 607.0 6 1722.5 7 2838.0 8 2838.0 9 2838.0 10 4278.5 11 5719.0 12 5719.0 13 5719.0 14 9122.0 15 12525.0 16 12525.0 17 12525.0 18 21701.5 19 30878.0 20 30878.0 21 30878.0 22 48287.5 23 65697.0 24 65697.0 25 65697.0 26 93705.5 27 121714.0 28 121714.0 29 121714.0 30 156664.0 31 191614.0 32 191614.0 33 191614.0 34 214967.0 35 238320.0 36 238320.0 37 238320.0 38 251284.5 39 264249.0 40 264249.0 41 264249.0 42 273282.0 43 282315.0 44 282315.0 45 282315.0 46 284067.5 47 285820.0 48 285820.0 49 285820.0 50 270414.5 51 255009.0 52 255009.0 53 255009.0 54 221082.0 55 187155.0 56 187155.0 57 187155.0 58 157770.5 59 128386.0 60 128386.0 61 128386.0 62 103217.5 63 78049.0 64 78049.0 65 78049.0 66 59303.5 67 40558.0 68 40558.0 69 40558.0 70 29326.5 71 18095.0 72 18095.0 73 18095.0 74 12487.5 75 6880.0 76 6880.0 77 6880.0 78 4673.5 79 2467.0 80 2467.0 81 2467.0 82 1591.5 83 716.0 84 716.0 85 716.0 86 469.0 87 222.0 88 222.0 89 222.0 90 147.5 91 73.0 92 73.0 93 73.0 94 45.0 95 17.0 96 17.0 97 17.0 98 11.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.462694714994541 3 0.0 4 0.0 5 4.5041636488770217E-4 6 9.008327297754043E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 5.855412743540128E-4 18 0.0 19 2.2520818244385109E-4 20 5.855412743540128E-4 21 7.206661838203236E-4 22 0.004684330194832102 23 3.152914554213915E-4 24 0.002207040187949741 25 8.557910932866342E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2220168.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.51085837852534 #Duplication Level Percentage of deduplicated Percentage of total 1 85.31529294911638 62.71600417504896 2 10.542650802802163 15.499986201980725 3 2.3178512227385135 5.111616989316679 4 0.7612849971607205 2.2385085444791115 5 0.3297354076286203 1.2119566426286414 6 0.1752532254110037 0.7729809020140842 7 0.10608838915675595 0.5459053985635719 8 0.07420687633716891 0.43640089417074934 9 0.05704897054182252 0.3774346914726522 >10 0.2806879104222997 3.6666913604862086 >50 0.020120073295526595 1.03759472123258 >100 0.016247926454818564 2.44145686734988 >500 0.001858552070660031 0.9736264411552839 >1k 0.0014868416565268835 2.171498010530933 >5k 1.8585520706586043E-4 0.7983381595698011 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6751 0.30407608793568774 No Hit GTCCTACAGTGGACATTTCTAAATT 5615 0.2529087888844448 No Hit CTGTAGGACGTGGAATATGGCAAGA 5164 0.23259501082800943 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4834 0.21773127078671523 No Hit GGTATCAACGCAGAGTACTTTTTTT 4305 0.1939042450841558 No Hit CTTTAGGACGTGAAATATGGCGAGG 4037 0.1818330865051654 No Hit TATCAACGCAGAGTACTTTTTTTTT 3991 0.17976117122668195 No Hit GTCCTACAGTGTGCATTTCTCATTT 2734 0.12314383416029777 No Hit CTGAAGGACCTGGAATATGGCGAGA 2274 0.10242468137546348 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.504163648877022E-5 0.0 0.0 0.0 3 0.0 4.504163648877022E-5 0.0 0.0 0.0 4 0.0 4.504163648877022E-5 0.0 0.0 0.0 5 0.0 9.008327297754045E-5 0.0 0.0 0.0 6 0.0 9.008327297754045E-5 0.0 4.504163648877022E-5 0.0 7 0.0 9.008327297754045E-5 0.0 4.504163648877022E-5 0.0 8 0.0 9.008327297754045E-5 0.0 4.504163648877022E-5 0.0 9 0.0 9.008327297754045E-5 0.0 4.504163648877022E-5 0.0 10 0.0 9.008327297754045E-5 0.0 9.008327297754045E-5 0.0 11 0.0 9.008327297754045E-5 0.0 2.2520818244385109E-4 0.0 12 0.0 9.008327297754045E-5 0.0 2.2520818244385109E-4 0.0 13 0.0 9.008327297754045E-5 0.0 2.2520818244385109E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAGGA 80 1.1348257E-6 13.737797 2 TAGGACC 870 0.0 13.679312 4 GGTATCA 1275 0.0 13.24314 1 CGGTAGG 115 2.5156623E-9 13.031902 1 TCCAACG 125 1.1277734E-10 12.846683 18 GTCCTAG 300 0.0 11.98935 1 ACCGAGC 65 8.426086E-4 11.624649 8 GTCGAGG 165 7.2759576E-12 11.449805 19 GGCGAGG 1240 0.0 11.350543 19 TGGACCG 75 2.1889774E-4 11.334287 5 TATGTCG 210 0.0 11.245091 16 GAACAGT 355 0.0 11.174649 6 GGACCTG 1790 0.0 11.081006 6 ATGTCGA 205 0.0 11.058837 17 TAGAGCG 60 0.0061353347 11.019445 5 TGTCGAG 225 0.0 10.915482 18 AGGACCT 1880 0.0 10.801737 5 CGTAAGA 65 0.007325452 10.759674 2 CGTGCGC 115 9.5053656E-7 10.676986 10 GTATCAA 2665 0.0 10.609713 1 >>END_MODULE