FastQCFastQC Report
Thu 26 May 2016
SRR1511798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511798_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220168
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85380.38456549234112014No Hit
GTCCTACAGTGGACATTTCTAAATT60390.27200644275568336No Hit
GGTATCAACGCAGAGTACTTTTTTT56580.2548455792534619No Hit
CTGTAGGACGTGGAATATGGCAAGA56160.25295383052093356No Hit
TATCAACGCAGAGTACTTTTTTTTT53150.2393962979378137No Hit
GTCCTAAAGTGTGTATTTCTCATTT50180.22601893190064898No Hit
CTTTAGGACGTGAAATATGGCGAGG47170.2124613993175291No Hit
GTCCTACAGTGTGCATTTCTCATTT29980.13503482619333312No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27230.12264837615892131No Hit
CTGAAGGACCTGGAATATGGCGAGA26270.11832437905599937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.013.3466791
TAGGACC9650.012.994854
GACCGTG853.9582974E-612.2905477
GTGCGCC701.09426954E-412.21073911
AATGTCC7200.011.3439268
TAGGACT4450.011.3146334
CTAGGAC3650.011.1918083
TATAGCG600.005876897411.083365
GGCGAGG11800.010.62451119
CCGTTTC1002.4073464E-510.44696513
AGGACCT19100.010.445055
TGTAGGA32750.010.4427732
CCAACGA1106.0655602E-610.361093519
CCCGTTT1106.0688217E-610.36062712
AAATGTC7650.010.3041967
GTATCAA31350.010.2832191
GGACCTG18550.010.1372176
TAGAAAT8250.010.1333574
ACCGTGC750.002654723110.1303918
GTAGGAC32750.010.0656733
Produced by FastQC (version 0.11.5)