##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511798_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2220168 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.060072481001438 31.0 31.0 34.0 28.0 34.0 2 31.255470306751562 33.0 31.0 34.0 28.0 34.0 3 31.373416336061055 33.0 31.0 34.0 28.0 34.0 4 34.94764089924726 37.0 35.0 37.0 32.0 37.0 5 34.6151764190818 37.0 35.0 37.0 32.0 37.0 6 34.562815516663605 37.0 35.0 37.0 31.0 37.0 7 34.41471140922669 37.0 35.0 37.0 30.0 37.0 8 34.335636762623366 37.0 35.0 37.0 30.0 37.0 9 35.866962770384944 39.0 35.0 39.0 30.0 39.0 10 35.682419979028616 39.0 35.0 39.0 30.0 39.0 11 35.66574601561683 39.0 35.0 39.0 30.0 39.0 12 35.507945795092986 39.0 35.0 39.0 30.0 39.0 13 35.50126296748714 39.0 35.0 39.0 30.0 39.0 14 36.560252647547394 40.0 36.0 41.0 30.0 41.0 15 36.505553633779066 40.0 36.0 41.0 30.0 41.0 16 36.406132328724674 40.0 36.0 41.0 30.0 41.0 17 36.36400984069674 40.0 36.0 41.0 30.0 41.0 18 36.299186818294835 40.0 36.0 41.0 29.0 41.0 19 36.32186618309966 40.0 36.0 41.0 29.0 41.0 20 36.20034024452204 40.0 36.0 41.0 29.0 41.0 21 36.09324159252813 39.0 36.0 41.0 29.0 41.0 22 36.189131633281804 40.0 36.0 41.0 29.0 41.0 23 36.08735374980632 40.0 36.0 41.0 29.0 41.0 24 35.97556851553576 40.0 36.0 41.0 29.0 41.0 25 35.55265097055718 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4591051536251811 1101 2 0.4189338471992947 1101 3 0.5368097039216906 1101 4 0.41111050130218274 1101 5 0.5138395666850286 1101 6 0.49022649053573275 1101 7 0.5149974756761608 1101 8 0.5494903978619661 1101 9 0.6421262934238925 1101 10 0.5100821627370209 1101 11 0.36551496062975275 1101 12 0.6192217843763999 1101 13 0.5405437616774336 1101 14 0.33840812351476046 1101 15 0.1867724382917757 1101 16 0.40286615376664514 1101 17 0.520269198998065 1101 18 0.6262282754196704 1101 19 0.6919030596854938 1101 20 0.7422034946603375 1101 21 0.8442397253706915 1101 22 0.6937286904469246 1101 23 0.35056087076796416 1101 24 0.39151682964230616 1101 25 0.40634772451659273 1102 1 0.43208221361398813 1102 2 0.3518392291043604 1102 3 0.48890636348103556 1102 4 0.4626945264651212 1102 5 0.498362242061944 1102 6 0.4581815480747835 1102 7 0.6337865092317898 1102 8 0.5810000229886114 1102 9 0.49459646536055857 1102 10 0.5852473701346881 1102 11 0.7465051464786256 1102 12 0.8272984224625688 1102 13 0.8313118059545275 1102 14 1.0022636180784161 1102 15 0.9872472677916946 1102 16 0.9932213685740621 1102 17 1.072854297820065 1102 18 1.088716744926245 1102 19 1.070984854359537 1102 20 1.029584220211909 1102 21 1.0711053970124809 1102 22 0.849941547136126 1102 23 1.1893033286911177 1102 24 1.0989734674260347 1102 25 1.2581510180791753 1103 1 0.49546150715125137 1103 2 0.4601204356397197 1103 3 0.49338471393559047 1103 4 0.5156674156067496 1103 5 0.6355732675874606 1103 6 0.65378295715999 1103 7 0.7317218695405856 1103 8 0.4327400856638519 1103 9 0.5214882311727678 1103 10 0.599818502597067 1103 11 0.7399877581604457 1103 12 0.7420157807478347 1103 13 0.5174646636354439 1103 14 0.7831196053365872 1103 15 0.7600977605037897 1103 16 0.6126750649610031 1103 17 0.5837102112268013 1103 18 0.8171168030226994 1103 19 0.7156654592519516 1103 20 0.7657184919506932 1103 21 0.676995786726458 1103 22 1.0191098077627316 1103 23 0.8880835493099397 1103 24 0.8685555422079858 1103 25 0.723941560665601 1104 1 0.43619459966063445 1104 2 0.4221754094741854 1104 3 0.41583530260046686 1104 4 0.32461613230873354 1104 5 0.46407558287234707 1104 6 0.38682446067939225 1104 7 0.36479044907017055 1104 8 0.416356477709229 1104 9 0.3660268855141311 1104 10 0.4824966727545359 1104 11 0.6241400931326382 1104 12 0.5608546298648633 1104 13 0.5742258484587737 1104 14 0.6938145340189479 1104 15 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0.7611413605137827 1107 15 0.6062710015817601 1107 16 0.8056224777821228 1107 17 0.701261442424908 1107 18 0.7964064385347029 1107 19 0.7808005300793042 1107 20 0.6423632213809256 1107 21 0.7463344430391814 1107 22 0.5891104566867327 1107 23 0.6625885419689865 1107 24 0.7135834774850309 1107 25 0.5810297936004716 1108 1 0.22707239948955404 1108 2 0.19750630414398884 1108 3 0.17975090511531988 1108 4 0.2645440607882108 1108 5 0.21924371290718625 1108 6 0.3129707090659437 1108 7 0.3866712470607325 1108 8 0.1509617764455058 1108 9 0.2257267802468519 1108 10 0.2601552666626361 1108 11 0.3315191760659033 1108 12 0.3328672245246338 1108 13 0.4461726298140505 1108 14 0.509169951372229 1108 15 0.5581891163599764 1108 16 0.41055570307982236 1108 17 0.35687857186027827 1108 18 0.3719835529865563 1108 19 0.45196345649449654 1108 20 0.4641988786057283 1108 21 0.5084056565269108 1108 22 0.45371262993923267 1108 23 0.4529282754489401 1108 24 0.6404681124708915 1108 25 0.656965419154119 1109 1 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-0.5246463367911929 2313 15 -0.5691379885889418 2313 16 -0.48406056540927267 2313 17 -0.4426802585005021 2313 18 -0.4568996005907522 2313 19 -0.42946144356261584 2313 20 -0.4869684652770516 2313 21 -0.4626488195209504 2313 22 -0.43952096999955614 2313 23 -0.3383567455506764 2313 24 -0.2908814376153259 2313 25 -0.25767426725884235 2314 1 -0.44061347047039234 2314 2 -0.4653669031234777 2314 3 -0.5788443948288347 2314 4 -0.5528288936987522 2314 5 -0.5279373013275261 2314 6 -0.5633986034151732 2314 7 -0.7095960784062783 2314 8 -0.7245064439085596 2314 9 -0.6793455800787811 2314 10 -0.8690250633184888 2314 11 -0.7705131045635127 2314 12 -0.8226592421532715 2314 13 -0.7618516506770305 2314 14 -0.8861099195350874 2314 15 -0.6908866496361483 2314 16 -0.8896361456221484 2314 17 -0.740724370497972 2314 18 -0.7515991554030776 2314 19 -0.7307681936925547 2314 20 -0.7131836920515866 2314 21 -0.7688989607606942 2314 22 -0.765844103940033 2314 23 -0.8437266806933863 2314 24 -0.8876871595199134 2314 25 -0.8972615082251494 2315 1 -0.5677218418774963 2315 2 -0.6489919488393809 2315 3 -0.6695097330804174 2315 4 -0.7104901876238472 2315 5 -0.6071044231118137 2315 6 -0.7204089950833961 2315 7 -0.8466966712648301 2315 8 -0.8466568912399453 2315 9 -1.088079054265613 2315 10 -1.0039547811494032 2315 11 -1.1384978206810743 2315 12 -0.8841753415008142 2315 13 -1.0713687149219595 2315 14 -1.228380610142814 2315 15 -1.2475397871810046 2315 16 -1.3346841152595843 2315 17 -1.3403452707287897 2315 18 -1.3994399957514645 2315 19 -1.2827942620613584 2315 20 -1.3778306932179376 2315 21 -1.4250776039616397 2315 22 -1.3189454507327483 2315 23 -1.500322062731371 2315 24 -1.317331355048239 2315 25 -1.354652555948661 2316 1 -1.4615708857592509 2316 2 -1.1423669405128507 2316 3 -1.0301505427593796 2316 4 -0.8570516438079778 2316 5 -0.9854517241673975 2316 6 -0.7553386755453886 2316 7 -0.9782664481603902 2316 8 -0.8862482644613081 2316 9 -1.2214259815363988 2316 10 -1.211853013947234 2316 11 -0.9225584151239872 2316 12 -1.1631379185157513 2316 13 -1.0809236433981653 2316 14 -1.3445388179770532 2316 15 -1.248480066841843 2316 16 -1.3134810634303165 2316 17 -1.499310855475116 2316 18 -1.3787682419270055 2316 19 -1.372533883860953 2316 20 -1.3621507844854222 2316 21 -1.636954535970915 2316 22 -1.5651287006941317 2316 23 -1.4391426538814827 2316 24 -1.333994303259118 2316 25 -1.2856690440761227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29572.0 3 2.0 4 554.0 5 4851.0 6 6148.0 7 5092.0 8 4040.0 9 3725.0 10 3392.0 11 3239.0 12 3149.0 13 3059.0 14 2939.0 15 3120.0 16 3021.0 17 3232.0 18 3499.0 19 4004.0 20 4808.0 21 6186.0 22 8273.0 23 10026.0 24 12442.0 25 14947.0 26 17278.0 27 19796.0 28 23184.0 29 28601.0 30 36826.0 31 46910.0 32 61491.0 33 80295.0 34 106369.0 35 142980.0 36 212800.0 37 386231.0 38 914087.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02864532807263 19.97633768725315 13.163880058486436 24.831136926187785 2 14.582635187967757 21.065568011069434 41.74584986361393 22.605946937348886 3 18.63286922431095 25.54459842678572 30.361576241077255 25.460956107826078 4 12.235830801993362 17.628080397519465 39.091546225330696 31.044542575156477 5 12.352767053520864 38.58105803623925 36.43956572729235 12.626609182947538 6 30.666913494834624 38.39137398611277 18.368654984667828 12.573057534384786 7 27.24487516260031 31.99469589688708 22.889168747590272 17.871260192922335 8 24.14803744581491 38.37142054114824 20.8924279604066 16.58811405263025 9 26.30859466490824 15.041249130696416 20.888329171486124 37.761827032909224 10 14.547592794779494 29.464436925493924 35.39042991341196 20.59754036631462 11 33.60844764900674 22.4061422378847 25.75381682827606 18.2315932848325 12 23.19288450243405 24.99666691889983 32.67342831713636 19.13702026152976 13 28.80466703420642 22.529601363500422 26.210944397000585 22.454787205292572 14 20.0184400459785 23.183515842044386 28.419110625862547 28.378933486114565 15 22.08058128934387 32.51330529941878 23.820539706905063 21.585573704332283 16 20.95251350348262 28.83943917757575 29.21567196716645 20.99237535177518 17 20.784913574107904 29.5219100536536 28.743365366945206 20.94981100529329 18 20.564524846768354 27.715695388817423 31.570133431343933 20.14964633307029 19 22.84489281892181 27.151503850159088 28.592701092890266 21.410902238028832 20 22.95781220159916 27.76515110568209 29.37345281978661 19.90358387293214 21 22.046574853794848 27.147450102875098 28.266554603075082 22.539420440254972 22 22.391999164027226 28.941953942224192 28.47099859109761 20.19504830265097 23 21.07669329528216 28.66147967180862 29.210266970787796 21.051560062121425 24 22.38900965650447 27.126543540754337 30.004126018329252 20.48032078441194 25 21.734536185791427 28.04496238346034 29.45210878280778 20.76839264794045 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 197.0 1 197.0 2 444.5 3 692.0 4 692.0 5 692.0 6 1888.0 7 3084.0 8 3084.0 9 3084.0 10 4456.0 11 5828.0 12 5828.0 13 5828.0 14 9058.0 15 12288.0 16 12288.0 17 12288.0 18 21725.5 19 31163.0 20 31163.0 21 31163.0 22 49008.0 23 66853.0 24 66853.0 25 66853.0 26 95285.5 27 123718.0 28 123718.0 29 123718.0 30 158518.0 31 193318.0 32 193318.0 33 193318.0 34 216635.0 35 239952.0 36 239952.0 37 239952.0 38 251810.5 39 263669.0 40 263669.0 41 263669.0 42 273350.5 43 283032.0 44 283032.0 45 283032.0 46 284145.5 47 285259.0 48 285259.0 49 285259.0 50 269937.5 51 254616.0 52 254616.0 53 254616.0 54 219901.5 55 185187.0 56 185187.0 57 185187.0 58 156125.0 59 127063.0 60 127063.0 61 127063.0 62 101894.0 63 76725.0 64 76725.0 65 76725.0 66 58298.0 67 39871.0 68 39871.0 69 39871.0 70 28791.5 71 17712.0 72 17712.0 73 17712.0 74 12217.5 75 6723.0 76 6723.0 77 6723.0 78 4523.5 79 2324.0 80 2324.0 81 2324.0 82 1493.5 83 663.0 84 663.0 85 663.0 86 420.5 87 178.0 88 178.0 89 178.0 90 112.0 91 46.0 92 46.0 93 46.0 94 25.5 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4079421016787919 2 0.0 3 0.0 4 0.0 5 0.02887168898930171 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004954580013764724 25 4.504163648877022E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2220168.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.34944893295132 #Duplication Level Percentage of deduplicated Percentage of total 1 84.21453273141086 60.086605025321575 2 11.157779476905548 15.922028339852092 3 2.6094014574269275 5.585380680967539 4 0.8636131371893618 2.46473285718873 5 0.34774866460643666 1.2405837793419483 6 0.19263810695847836 0.8246773664984618 7 0.12034580033691339 0.601062457480363 8 0.07769303516734839 0.44346841960949723 9 0.058519773410589615 0.3757818226074071 >10 0.31336746892586925 3.998630464370841 >50 0.022283588413900844 1.099728032118541 >100 0.01810638438872717 2.6650126070126725 >500 0.0020166983410685358 1.0725224376615803 >1k 0.0015755455789592714 1.9927459697231809 >5k 3.7813093895022515E-4 1.6270397402455845 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8538 0.38456549234112014 No Hit GTCCTACAGTGGACATTTCTAAATT 6039 0.27200644275568336 No Hit GGTATCAACGCAGAGTACTTTTTTT 5658 0.2548455792534619 No Hit CTGTAGGACGTGGAATATGGCAAGA 5616 0.25295383052093356 No Hit TATCAACGCAGAGTACTTTTTTTTT 5315 0.2393962979378137 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5018 0.22601893190064898 No Hit CTTTAGGACGTGAAATATGGCGAGG 4717 0.2124613993175291 No Hit GTCCTACAGTGTGCATTTCTCATTT 2998 0.13503482619333312 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2723 0.12264837615892131 No Hit CTGAAGGACCTGGAATATGGCGAGA 2627 0.11832437905599937 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.504163648877022E-5 0.0 0.0 0.0 0.0 2 4.504163648877022E-5 0.0 0.0 0.0 0.0 3 4.504163648877022E-5 0.0 0.0 0.0 0.0 4 4.504163648877022E-5 0.0 0.0 9.008327297754045E-5 0.0 5 4.504163648877022E-5 0.0 0.0 9.008327297754045E-5 0.0 6 4.504163648877022E-5 0.0 0.0 1.3512490946631067E-4 0.0 7 4.504163648877022E-5 0.0 0.0 1.3512490946631067E-4 0.0 8 4.504163648877022E-5 0.0 0.0 1.3512490946631067E-4 0.0 9 4.504163648877022E-5 0.0 0.0 1.801665459550809E-4 0.0 10 4.504163648877022E-5 0.0 0.0 1.801665459550809E-4 0.0 11 4.504163648877022E-5 0.0 0.0 1.801665459550809E-4 0.0 12 4.504163648877022E-5 0.0 0.0 2.2520818244385109E-4 0.0 13 4.504163648877022E-5 0.0 0.0 2.2520818244385109E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1515 0.0 13.346679 1 TAGGACC 965 0.0 12.99485 4 GACCGTG 85 3.9582974E-6 12.290547 7 GTGCGCC 70 1.09426954E-4 12.210739 11 AATGTCC 720 0.0 11.343926 8 TAGGACT 445 0.0 11.314633 4 CTAGGAC 365 0.0 11.191808 3 TATAGCG 60 0.0058768974 11.08336 5 GGCGAGG 1180 0.0 10.624511 19 CCGTTTC 100 2.4073464E-5 10.446965 13 AGGACCT 1910 0.0 10.44505 5 TGTAGGA 3275 0.0 10.442773 2 CCAACGA 110 6.0655602E-6 10.3610935 19 CCCGTTT 110 6.0688217E-6 10.360627 12 AAATGTC 765 0.0 10.304196 7 GTATCAA 3135 0.0 10.283219 1 GGACCTG 1855 0.0 10.137217 6 TAGAAAT 825 0.0 10.133357 4 ACCGTGC 75 0.0026547231 10.130391 8 GTAGGAC 3275 0.0 10.065673 3 >>END_MODULE