FastQCFastQC Report
Thu 26 May 2016
SRR1511797_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511797_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992613
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54790.2749655853896366No Hit
TATCAACGCAGAGTACTTTTTTTTT36270.18202229936269612No Hit
GGTATCAACGCAGAGTACTTTTTTT35810.17971377281991033No Hit
GTCCTACAGTGGACATTTCTAAATT29650.14879959129043122No Hit
GTCCTAAAGTGTGTATTTCTCATTT28420.14262679205646053No Hit
CTGTAGGACGTGGAATATGGCAAGA26850.13474769059521344No Hit
CTTTAGGACGTGAAATATGGCGAGG23980.1203444923826152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6500.013.6593184
GGTATCA11600.013.6092291
CGTTAGG758.796736E-613.3221141
GCGTTGA550.002101903512.7165631
GTACCGT658.4235845E-411.624956
AGGACCT11600.011.4808925
GCACCGA600.006134811.0194846
GCGAACC1053.7156206E-610.79459711
TGTAGGA19250.010.6922952
GTCCTAA9750.010.6576911
CGGTAGG854.1430164E-410.5793261
CTGTAGG19500.010.4527361
CGAACCG1002.5572052E-510.38979912
GTAGGAC20250.010.3548173
GTGGTAT3400.010.2854561
GGCGAGG8300.010.24237619
GGACCTG12400.010.1308166
GTATCAA22550.010.1023571
GTATTAT2700.09.9915861
GGACCTA1054.374778E-59.8950476
Produced by FastQC (version 0.11.5)