FastQCFastQC Report
Thu 26 May 2016
SRR1511797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511797_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992613
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69750.3500428833898002No Hit
GGTATCAACGCAGAGTACTTTTTTT49300.2474138229550846No Hit
TATCAACGCAGAGTACTTTTTTTTT47550.2386313850205735No Hit
GTCCTACAGTGGACATTTCTAAATT33180.16651502323833078No Hit
GTCCTAAAGTGTGTATTTCTCATTT30560.153366459016377No Hit
CTGTAGGACGTGGAATATGGCAAGA29930.150204781359953No Hit
CTTTAGGACGTGAAATATGGCGAGG27270.1368554756994961No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22840.11462336138527653No Hit
GTCCTACAGTGTGCATTTCTCATTT21340.10709555744140985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.014.1308811
TAGGACC6100.011.5246364
GGCGAGG7500.011.14419319
GCCCTAT951.3011957E-511.0443991
TAGGACT3200.010.9844194
GTGGTAT4150.010.8024621
ATAGGAC2750.010.7091333
CGAAATC1252.2499444E-710.63683813
GTCCTAT1803.274181E-1110.598161
AGGACGG1451.4437319E-810.48280055
AATGCCG1553.6779966E-910.41678118
CGCCCTA1106.0683014E-610.36055716
TAGGACA7000.010.3143274
TCCAACG1303.8552753E-710.22824318
TGTAGGA19500.010.1333742
TCTATAC2350.010.1064233
GTATAGA2950.010.0233451
GTATCAA25400.09.76365951
GTAGGAC19300.09.7461523
TTAGGAC11550.09.7056673
Produced by FastQC (version 0.11.5)