##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511797_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1992613 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.161024243041673 31.0 31.0 34.0 28.0 34.0 2 31.355703791955587 33.0 31.0 34.0 28.0 34.0 3 31.469456437351358 33.0 31.0 34.0 28.0 34.0 4 35.04551209893742 37.0 35.0 37.0 32.0 37.0 5 34.71709609442476 37.0 35.0 37.0 32.0 37.0 6 34.66388255019916 37.0 35.0 37.0 32.0 37.0 7 34.51561793484234 37.0 35.0 37.0 31.0 37.0 8 34.43854125211469 37.0 35.0 37.0 30.0 37.0 9 35.993246054301565 39.0 37.0 39.0 31.0 39.0 10 35.81828985357418 39.0 35.0 39.0 30.0 39.0 11 35.79877226536212 39.0 35.0 39.0 30.0 39.0 12 35.642229073081424 39.0 35.0 39.0 30.0 39.0 13 35.63153356923798 39.0 35.0 39.0 30.0 39.0 14 36.7171578224171 40.0 36.0 41.0 30.0 41.0 15 36.66009405740101 40.0 36.0 41.0 30.0 41.0 16 36.56686069999543 40.0 36.0 41.0 30.0 41.0 17 36.51885840351338 40.0 36.0 41.0 30.0 41.0 18 36.4520441249756 40.0 36.0 41.0 30.0 41.0 19 36.467889148570244 40.0 36.0 41.0 30.0 41.0 20 36.34924945285412 40.0 36.0 41.0 29.0 41.0 21 36.24324893995974 40.0 36.0 41.0 29.0 41.0 22 36.32975695732187 40.0 36.0 41.0 30.0 41.0 23 36.232260353616084 40.0 36.0 41.0 30.0 41.0 24 36.123311952697286 40.0 36.0 41.0 29.0 41.0 25 35.70741684411374 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38266051861131345 1101 2 0.309527833611142 1101 3 0.345234219096735 1101 4 0.274966868765091 1101 5 0.38842442505772823 1101 6 0.4164291581333899 1101 7 0.4845514515203888 1101 8 0.4847846650746135 1101 9 0.4896544285855242 1101 10 0.5133978071000698 1101 11 0.3131316814339655 1101 12 0.5710740946794672 1101 13 0.6700143710415958 1101 14 0.6975773165396149 1101 15 0.4968326291918004 1101 16 0.45606056649864257 1101 17 0.5247540820434722 1101 18 0.5134062880854273 1101 19 0.5660473000900339 1101 20 0.673437738306319 1101 21 0.5965143299736368 1101 22 0.4281519472434141 1101 23 0.18743534074366153 1101 24 0.27633510097165725 1101 25 0.2955841251706488 1102 1 0.4838210867118953 1102 2 0.4720541286329585 1102 3 0.4160443748042617 1102 4 0.38018859248865056 1102 5 0.5219096140103616 1102 6 0.453528519366138 1102 7 0.5750825533106294 1102 8 0.5820134708386391 1102 9 0.5486981225204417 1102 10 0.5766282065794215 1102 11 0.5271853296840803 1102 12 0.4572086431993867 1102 13 0.5737752317503606 1102 14 0.6891731585311973 1102 15 0.8623494464756689 1102 16 0.7428037759133517 1102 17 0.8016505904745372 1102 18 0.9742046062289234 1102 19 1.030485284834434 1102 20 1.0115417551351271 1102 21 1.0157402506517101 1102 22 0.8071141362191057 1102 23 0.8510858200112708 1102 24 0.7121099105329236 1102 25 0.8617527771488227 1103 1 0.4041396550077856 1103 2 0.4351071357495826 1103 3 0.3917504277134469 1103 4 0.3244298436058486 1103 5 0.3803854099352506 1103 6 0.5309631514426272 1103 7 0.39041886667502723 1103 8 0.2748493100508398 1103 9 0.5436302722707183 1103 10 0.6252381039022268 1103 11 0.7205935604703768 1103 12 0.6203104910913595 1103 13 0.42407205722392405 1103 14 0.6778827579748565 1103 15 0.7348365947817967 1103 16 0.47898363392801 1103 17 0.672528720343891 1103 18 0.9451768847502677 1103 19 0.7884560002030412 1103 20 0.6486216345951021 1103 21 0.5674770732493641 1103 22 0.6466988408749685 1103 23 0.7324287677875887 1103 24 0.8708647434763961 1103 25 0.9054578334086898 1104 1 0.4278958505611783 1104 2 0.3780476646072195 1104 3 0.42323789821763214 1104 4 0.4301546539379615 1104 5 0.4752797362495329 1104 6 0.3578516989131515 1104 7 0.4748167818487232 1104 8 0.4816263177274607 1104 9 0.6782537086730258 1104 10 0.5656576606518655 1104 11 0.6656425387274325 1104 12 0.5284660121524993 1104 13 0.7086272246437986 1104 14 0.8520202285231164 1104 15 0.8121590403362973 1104 16 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-0.4510564902855769 2315 2 -0.4487343239572077 2315 3 -0.4693015898705397 2315 4 -0.44217980326433093 2315 5 -0.5446270090606191 2315 6 -0.5149303005891781 2315 7 -0.5839846707040479 2315 8 -0.7175316009000525 2315 9 -0.8054691049552289 2315 10 -0.6790237867600908 2315 11 -0.9494068230918273 2315 12 -0.7216419995838663 2315 13 -0.8126071079576036 2315 14 -0.9040755983956004 2315 15 -0.9286469733196725 2315 16 -0.9920716860330359 2315 17 -1.0188950157952448 2315 18 -1.1008489430190735 2315 19 -1.0231908662340459 2315 20 -1.1342919787760621 2315 21 -1.2195486076437163 2315 22 -1.1914687795518688 2315 23 -1.170599221962874 2315 24 -1.0276224778212253 2315 25 -1.1873375586059183 2316 1 -1.589890406779677 2316 2 -1.2411120897204029 2316 3 -1.22149190381554 2316 4 -0.9497180244612622 2316 5 -0.8375552678994751 2316 6 -1.0038326370697845 2316 7 -1.0331320075708348 2316 8 -1.0337474291607833 2316 9 -1.404518147294013 2316 10 -1.2230650544316077 2316 11 -1.0357748512117837 2316 12 -1.220853967470923 2316 13 -1.190832283306591 2316 14 -1.3874478657858589 2316 15 -1.396547142803044 2316 16 -1.4324439228201058 2316 17 -1.4393813733308463 2316 18 -1.5767587685371822 2316 19 -1.5269874832701191 2316 20 -1.4564466472925233 2316 21 -1.6720845190285374 2316 22 -1.5074299455625138 2316 23 -1.4852138476640206 2316 24 -1.3074481999471814 2316 25 -1.4983213345439452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24431.0 3 0.0 4 444.0 5 4019.0 6 5351.0 7 4252.0 8 3476.0 9 3087.0 10 2905.0 11 2943.0 12 2753.0 13 2558.0 14 2606.0 15 2520.0 16 2722.0 17 2762.0 18 3098.0 19 3545.0 20 4269.0 21 5366.0 22 6860.0 23 8790.0 24 10762.0 25 13006.0 26 15260.0 27 17441.0 28 20293.0 29 24724.0 30 32131.0 31 41573.0 32 53710.0 33 71334.0 34 93352.0 35 125174.0 36 185428.0 37 335764.0 38 853904.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20317381284392 19.58256333086563 13.223850615405627 24.990412240884826 2 14.995686568340163 20.949778005061695 40.459035447425066 23.595499979173077 3 18.291710432482375 25.381546742894884 30.41262904537911 25.914113779243635 4 12.538561175702457 17.48994912710095 38.39696920576148 31.574520491435116 5 12.979529467396688 38.04464974601962 35.9975964856886 12.978224300895095 6 31.223273159414294 37.797254158233436 18.07746913223993 12.90200355011234 7 27.377167568413935 31.928026164639096 22.806485755136595 17.88832051181037 8 24.84882915046725 36.96814183185596 20.802734901358168 17.380294116318623 9 26.37145296151335 14.894312141896094 20.469353557364126 38.264881339226434 10 14.922717055444284 28.95564768472353 34.88218735901051 21.239447900821684 11 33.92414884375441 22.32325092729998 25.07019677177656 18.682403457169052 12 22.991067507840206 24.862128270768082 32.14683433260749 19.999969888784225 13 28.905462325097748 22.030820836760576 26.05257518645116 23.01114165169052 14 20.839570955323488 22.20762385872219 27.948176590236034 29.004628595718284 15 22.809095393837136 30.99728848501942 23.707262775059686 22.48635334608376 16 21.976481121261944 28.102510674431347 27.933586669155865 21.987421535150848 17 21.487012279855648 28.64871402525227 27.980897444712046 21.88337625018004 18 21.371557218142428 27.30894288950528 30.14933672452877 21.170163167823524 19 23.228494444229764 26.8604089203473 28.068270155820525 21.84282647960241 20 23.366203071042897 27.11901407849894 28.525258040572854 20.989524809885314 21 22.74902351836508 26.670808631681115 27.775538953123363 22.804628896830444 22 23.008180715472598 27.812726304606063 27.871342804648968 21.307750175272368 23 22.198540308629923 27.584934957264657 28.263240277966673 21.95328445613875 24 22.900780215665943 26.70529769749554 28.77714017282585 21.616781914012666 25 22.49727744014132 27.21897410933399 28.54693090971138 21.736817540813306 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 306.0 1 306.0 2 499.0 3 692.0 4 692.0 5 692.0 6 1796.0 7 2900.0 8 2900.0 9 2900.0 10 3993.5 11 5087.0 12 5087.0 13 5087.0 14 7720.0 15 10353.0 16 10353.0 17 10353.0 18 18362.0 19 26371.0 20 26371.0 21 26371.0 22 40572.5 23 54774.0 24 54774.0 25 54774.0 26 77980.0 27 101186.0 28 101186.0 29 101186.0 30 128187.5 31 155189.0 32 155189.0 33 155189.0 34 173865.0 35 192541.0 36 192541.0 37 192541.0 38 207176.0 39 221811.0 40 221811.0 41 221811.0 42 232358.0 43 242905.0 44 242905.0 45 242905.0 46 247745.0 47 252585.0 48 252585.0 49 252585.0 50 245246.0 51 237907.0 52 237907.0 53 237907.0 54 212379.0 55 186851.0 56 186851.0 57 186851.0 58 160604.5 59 134358.0 60 134358.0 61 134358.0 62 110173.0 63 85988.0 64 85988.0 65 85988.0 66 66088.0 67 46188.0 68 46188.0 69 46188.0 70 33999.0 71 21810.0 72 21810.0 73 21810.0 74 15171.0 75 8532.0 76 8532.0 77 8532.0 78 5782.0 79 3032.0 80 3032.0 81 3032.0 82 1976.0 83 920.0 84 920.0 85 920.0 86 592.0 87 264.0 88 264.0 89 264.0 90 159.0 91 54.0 92 54.0 93 54.0 94 31.0 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41714070920946517 2 0.0 3 0.0 4 0.0 5 0.02659824060166224 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 5.018535962577781E-5 17 0.0 18 1.0037071925155562E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004366126287442669 25 1.5055607887733344E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1992613.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.90947752511964 #Duplication Level Percentage of deduplicated Percentage of total 1 83.31270592091705 59.909731539770874 2 11.57144287703863 16.641928229992303 3 2.831683941259755 6.1087473819678175 4 0.9565335862377742 2.751353216863493 5 0.4294617149495352 1.5441183769531475 6 0.2337891662681618 1.0086994078426104 7 0.14124086958194473 0.7109589995781848 8 0.0991324548993839 0.5702850430077747 9 0.06669806226909317 0.4316600528737547 >10 0.3197876436604576 4.055225190852001 >50 0.01973520529872462 0.9894070341348346 >100 0.014580959661170664 2.058319229772672 >500 0.0020221813216058098 1.0028050455007849 >1k 0.0011156862464031746 1.8670155039839722 >5k 6.973039040019841E-5 0.34974574690573684 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6975 0.3500428833898002 No Hit GGTATCAACGCAGAGTACTTTTTTT 4930 0.2474138229550846 No Hit TATCAACGCAGAGTACTTTTTTTTT 4755 0.2386313850205735 No Hit GTCCTACAGTGGACATTTCTAAATT 3318 0.16651502323833078 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3056 0.153366459016377 No Hit CTGTAGGACGTGGAATATGGCAAGA 2993 0.150204781359953 No Hit CTTTAGGACGTGAAATATGGCGAGG 2727 0.1368554756994961 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2284 0.11462336138527653 No Hit GTCCTACAGTGTGCATTTCTCATTT 2134 0.10709555744140985 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.018535962577781E-5 0.0 8 0.0 0.0 0.0 5.018535962577781E-5 0.0 9 0.0 0.0 0.0 5.018535962577781E-5 0.0 10 0.0 0.0 0.0 5.018535962577781E-5 0.0 11 0.0 0.0 0.0 5.018535962577781E-5 0.0 12 0.0 0.0 0.0 5.018535962577781E-5 0.0 13 0.0 5.018535962577781E-5 0.0 5.018535962577781E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1215 0.0 14.130881 1 TAGGACC 610 0.0 11.524636 4 GGCGAGG 750 0.0 11.144193 19 GCCCTAT 95 1.3011957E-5 11.044399 1 TAGGACT 320 0.0 10.984419 4 GTGGTAT 415 0.0 10.802462 1 ATAGGAC 275 0.0 10.709133 3 CGAAATC 125 2.2499444E-7 10.636838 13 GTCCTAT 180 3.274181E-11 10.59816 1 AGGACGG 145 1.4437319E-8 10.4828005 5 AATGCCG 155 3.6779966E-9 10.416781 18 CGCCCTA 110 6.0683014E-6 10.360557 16 TAGGACA 700 0.0 10.314327 4 TCCAACG 130 3.8552753E-7 10.228243 18 TGTAGGA 1950 0.0 10.133374 2 TCTATAC 235 0.0 10.106423 3 GTATAGA 295 0.0 10.023345 1 GTATCAA 2540 0.0 9.7636595 1 GTAGGAC 1930 0.0 9.746152 3 TTAGGAC 1155 0.0 9.705667 3 >>END_MODULE