FastQCFastQC Report
Thu 26 May 2016
SRR1511796_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511796_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379436
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62130.26111229719984064No Hit
GTCCTACAGTGGACATTTCTAAATT56610.23791352236412328No Hit
CTGTAGGACGTGGAATATGGCAAGA50150.21076423152377285No Hit
GTCCTAAAGTGTGTATTTCTCATTT49750.20908316088350348No Hit
CTTTAGGACGTGAAATATGGCGAGG44780.18819585817815648No Hit
GGTATCAACGCAGAGTACTTTTTTT41400.17399081126788027No Hit
TATCAACGCAGAGTACTTTTTTTTT38950.16369425359623035No Hit
GTCCTACAGTGTGCATTTCTCATTT29130.12242396937761721No Hit
CTGAAGGACCTGGAATATGGCGAGA23920.1005280242881086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC350.002255784316.1921773
GCGGTAT400.003777138714.9869961
GGTATCA14200.013.0168741
CGGTAGG1153.5121047E-812.1633591
TAGGACC10900.012.0450984
CCAACGA1052.9323746E-711.69459519
GGCGAGG12650.011.49903419
TCTAGAC1850.011.232413
TCTATAC2450.011.1803133
TGTAGGA30050.010.6729362
CCGTAGA750.001741562610.6574191
CGTTAGG854.144666E-410.5790561
GTAGGAC32350.010.5695463
CACCTTT8850.010.5658614
CTGTAGG29550.010.4815991
TCCAACG1901.0913936E-1110.43991318
TGGCGAG28650.010.41822218
GTATCAA27700.010.3520291
GTCCTAA16850.010.3174571
AGGACGT32500.010.2301355
Produced by FastQC (version 0.11.5)