FastQCFastQC Report
Thu 26 May 2016
SRR1511796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511796_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379436
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79700.3349533250736729No Hit
GTCCTACAGTGGACATTTCTAAATT59550.2502693915701032No Hit
CTGTAGGACGTGGAATATGGCAAGA54160.22761696469247336No Hit
GGTATCAACGCAGAGTACTTTTTTT53910.226566295542305No Hit
GTCCTAAAGTGTGTATTTCTCATTT53350.22421279664592786No Hit
TATCAACGCAGAGTACTTTTTTTTT51850.2179087817449177No Hit
CTTTAGGACGTGAAATATGGCGAGG49810.2093353214795439No Hit
GTCCTACAGTGTGCATTTCTCATTT31440.13213215232517284No Hit
CTGAAGGACCTGGAATATGGCGAGA27230.11443888383633768No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24780.10414232616468777No Hit
CTGTAGGACCTGGAATATGGCGAGA24560.10321773731253961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.013.9830811
TAAGTCG500.001500843213.2989565
TAGGACC10000.012.34903054
TCCAACG1850.011.29569718
TGTAGGA33550.011.0423532
GGCGAGG13950.010.75833219
TTAGGAC19350.010.7510933
CTGTAGG32950.010.7347011
GCCGTGC909.542863E-510.5529528
AGGACGT32900.010.5386865
GTAGGAC33700.010.4858233
GGACGTG33000.010.4186416
TAGGACT4300.010.3829054
TAGGACA9250.010.1667694
GTCCTAA15650.010.1126561
AGGACCT19000.010.0992065
GACGTGA17450.010.0691497
GACGTGG16700.010.0663797
TAGGACG35550.09.9668684
GCTATTC1151.0278318E-59.9122653
Produced by FastQC (version 0.11.5)