##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511796_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2379436 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.163219351140356 31.0 31.0 34.0 28.0 34.0 2 31.361062873723018 33.0 31.0 34.0 28.0 34.0 3 31.476942014830406 33.0 31.0 34.0 28.0 34.0 4 35.04633240818412 37.0 35.0 37.0 32.0 37.0 5 34.72070818462863 37.0 35.0 37.0 32.0 37.0 6 34.67366804570495 37.0 35.0 37.0 32.0 37.0 7 34.531748279844464 37.0 35.0 37.0 31.0 37.0 8 34.45174949021533 37.0 35.0 37.0 30.0 37.0 9 36.00632544855167 39.0 37.0 39.0 31.0 39.0 10 35.83083344120203 39.0 35.0 39.0 30.0 39.0 11 35.80644110621172 39.0 35.0 39.0 30.0 39.0 12 35.6592490825557 39.0 35.0 39.0 30.0 39.0 13 35.65002210607892 39.0 35.0 39.0 30.0 39.0 14 36.730640370238994 40.0 37.0 41.0 30.0 41.0 15 36.67519025516971 40.0 36.0 41.0 30.0 41.0 16 36.584562896417474 40.0 36.0 41.0 30.0 41.0 17 36.53794848863344 40.0 36.0 41.0 30.0 41.0 18 36.47783466334039 40.0 36.0 41.0 30.0 41.0 19 36.49457560531151 40.0 36.0 41.0 30.0 41.0 20 36.375658349289495 40.0 36.0 41.0 30.0 41.0 21 36.272875589005125 40.0 36.0 41.0 29.0 41.0 22 36.3619681302628 40.0 36.0 41.0 30.0 41.0 23 36.2666064563199 40.0 36.0 41.0 30.0 41.0 24 36.159797531852085 40.0 36.0 41.0 29.0 41.0 25 35.74442851163049 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44557280062111815 1101 2 0.35079398232632286 1101 3 0.43046871146453825 1101 4 0.3151613147566934 1101 5 0.3107698950771294 1101 6 0.5241866932951353 1101 7 0.3807817377346794 1101 8 0.3468050957703781 1101 9 0.4269442334046971 1101 10 0.41756139964309824 1101 11 0.3721366717334149 1101 12 0.4009761803245624 1101 13 0.48249632079483007 1101 14 0.448246805518977 1101 15 0.17269624942660045 1101 16 0.28508108720600944 1101 17 0.41169720709127944 1101 18 0.30608792845023913 1101 19 0.4311624009870201 1101 20 0.42789345162916703 1101 21 0.49275682895978434 1101 22 0.5115126231541183 1101 23 0.27716172393313343 1101 24 0.24144210107332498 1101 25 0.43881042149973837 1102 1 0.5784448769790806 1102 2 0.5664571199650936 1102 3 0.6161147329282421 1102 4 0.4849112315143955 1102 5 0.6289392104635354 1102 6 0.6159383005117718 1102 7 0.7591525495892739 1102 8 0.6883292482858536 1102 9 0.7335398072531447 1102 10 0.9662950588185097 1102 11 0.791614589417641 1102 12 0.952496535432303 1102 13 1.0285611039027103 1102 14 1.1733178259525943 1102 15 1.171416266887995 1102 16 1.254794923022132 1102 17 1.2596750596293589 1102 18 1.2113184400089523 1102 19 1.266814602982386 1102 20 1.0950565653253364 1102 21 1.2654580331055314 1102 22 1.0373052624193164 1102 23 1.2120518830790417 1102 24 1.2391892694432514 1102 25 1.3592312321850457 1103 1 0.4271604302604253 1103 2 0.4341802340609888 1103 3 0.45012374815391354 1103 4 0.4654312524918893 1103 5 0.6454398760954803 1103 6 0.5590438610333166 1103 7 0.6222344998165639 1103 8 0.403636872160277 1103 9 0.642335491155464 1103 10 0.7744754312751496 1103 11 0.6363775416386446 1103 12 0.5657552443823448 1103 13 0.6629045275227767 1103 14 0.8477643535195227 1103 15 0.8886063446193688 1103 16 0.6347229880608509 1103 17 0.7516588126894632 1103 18 0.9283923771741414 1103 19 0.9421140311971143 1103 20 0.8698345287675977 1103 21 0.8101562268755558 1103 22 0.8855129395867607 1103 23 0.8445209211081774 1103 24 1.0112119200384981 1103 25 0.8773181613817655 1104 1 0.4746872252883989 1104 2 0.465958450648813 1104 3 0.5077759199022758 1104 4 0.4945442200246717 1104 5 0.5573955136312421 1104 6 0.6117489804154346 1104 7 0.6429518123718267 1104 8 0.6980545445522637 1104 9 0.7539916796485642 1104 10 0.7104600677485067 1104 11 0.7663977699073072 1104 12 0.6574790799319032 1104 13 0.7501486728483115 1104 14 0.859397816327693 1104 15 0.9268955100888547 1104 16 0.9912307649829586 1104 17 0.9340215933175458 1104 18 0.8910138451570262 1104 19 0.9465411231044385 1104 20 0.9170148173275834 1104 21 0.8828579414200775 1104 22 0.884670965005796 1104 23 0.9029717039397624 1104 24 1.0653665044836913 1104 25 1.1287497590777207 1105 1 0.0969462531009313 1105 2 0.23202678722364922 1105 3 0.20647295544863553 1105 4 0.2670734507891268 1105 5 0.20992216050358792 1105 6 0.28519390219450713 1105 7 0.2649788330549896 1105 8 0.25410541075747517 1105 9 0.3746648727766484 1105 10 0.5543105403175446 1105 11 0.35359423532912615 1105 12 0.36666376885806073 1105 13 0.3273372120064977 1105 14 0.44787439466482226 1105 15 0.48489651648767307 1105 16 0.34795103771160996 1105 17 0.3119092188409667 1105 18 0.372503905125825 1105 19 0.4127811759927553 1105 20 0.5847427808209886 1105 21 0.5897929759321059 1105 22 0.40849728245130024 1105 23 0.6483603682576486 1105 24 0.7688489780521479 1105 25 0.7703604317160426 1106 1 -0.0017486230702701278 1106 2 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1107 14 0.6085007916161302 1107 15 0.6277015900178498 1107 16 0.7286391326616197 1107 17 0.7438758730329695 1107 18 0.7958467210795135 1107 19 0.7256384273834939 1107 20 0.664981694080808 1107 21 0.6106149718595475 1107 22 0.4671283897606102 1107 23 0.6321643153163023 1107 24 0.5323966973928478 1107 25 0.44928734800730297 1108 1 0.19483656826980322 1108 2 0.15872401521304624 1108 3 0.24221172365299637 1108 4 0.16412379362588325 1108 5 0.10066151922534772 1108 6 0.1253300141806264 1108 7 0.10650088665067159 1108 8 0.12514478143516783 1108 9 0.0713250919759787 1108 10 0.204719871693527 1108 11 0.17980632916189165 1108 12 0.2660412415928093 1108 13 0.1401859607556304 1108 14 0.3839563908720436 1108 15 0.40058937537622086 1108 16 0.33772789856477914 1108 17 0.2787976616420309 1108 18 0.2952187347192421 1108 19 0.3857181911702341 1108 20 0.26264037873450974 1108 21 0.33148228516505185 1108 22 0.27299546731545377 1108 23 0.4408134284935201 1108 24 0.4432729870156322 1108 25 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-0.3186012899459669 2314 22 -0.4176380872909462 2314 23 -0.5091250725588239 2314 24 -0.44408048468449834 2314 25 -0.5543152111440932 2315 1 -0.7402893133831974 2315 2 -0.6985122407476005 2315 3 -0.7986700214716507 2315 4 -0.924295296379249 2315 5 -0.8743718092936987 2315 6 -0.8970003907601409 2315 7 -0.9204362239346295 2315 8 -0.9386164050898316 2315 9 -1.0246158718200036 2315 10 -0.9802878816819671 2315 11 -1.1032673597033877 2315 12 -1.0874146123097077 2315 13 -1.077903802470729 2315 14 -1.2572872874077419 2315 15 -1.2886414267727346 2315 16 -1.24925418694761 2315 17 -1.4609571248563356 2315 18 -1.4637917039571704 2315 19 -1.393627547563895 2315 20 -1.305123350445939 2315 21 -1.4263583120915229 2315 22 -1.519624175084246 2315 23 -1.4643729526682492 2315 24 -1.4797241666129395 2315 25 -1.520694087693137 2316 1 -1.528783094755429 2316 2 -1.1916087990017559 2316 3 -0.9217194645240703 2316 4 -0.7681153282993307 2316 5 -0.8771947934080799 2316 6 -0.8502867742807751 2316 7 -0.9959973062404828 2316 8 -0.9384815096570449 2316 9 -1.1135889712932396 2316 10 -1.1742881876516833 2316 11 -0.9995637823385266 2316 12 -1.1519861099901263 2316 13 -1.253638439896612 2316 14 -1.4295337666886354 2316 15 -1.3048629901952964 2316 16 -1.4458340450438172 2316 17 -1.4140145479297601 2316 18 -1.3470161906309528 2316 19 -1.4024143569613017 2316 20 -1.352989521772443 2316 21 -1.5758751693690556 2316 22 -1.5605357374317776 2316 23 -1.3578898058110482 2316 24 -1.2800706566441136 2316 25 -1.3848838468040015 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29207.0 3 1.0 4 537.0 5 4942.0 6 6195.0 7 4923.0 8 4116.0 9 3566.0 10 3478.0 11 3455.0 12 3189.0 13 3034.0 14 3092.0 15 2954.0 16 3116.0 17 3361.0 18 3467.0 19 4168.0 20 5098.0 21 6475.0 22 8291.0 23 10311.0 24 12715.0 25 15203.0 26 17884.0 27 20331.0 28 24173.0 29 29384.0 30 38225.0 31 49152.0 32 63828.0 33 84013.0 34 111391.0 35 149823.0 36 222541.0 37 405304.0 38 1018493.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.647933784554205 19.862650381754126 13.569245973889732 24.920169859801945 2 14.530796373594415 20.98951180027536 41.59220924622474 22.88748257990549 3 18.394274945827497 25.517139355712864 30.444819696768477 25.64376600169116 4 12.30392412319558 17.852886146128746 38.83899377835756 31.004195952318113 5 12.571790096729037 38.41181094749055 36.319294100831925 12.697104854948481 6 30.66407333502561 38.23490104377676 18.42461827088436 12.676407350313267 7 27.13037879564737 31.978628548950255 22.99263354845434 17.898359106948032 8 24.22153821325726 37.995474557836395 20.939373868429325 16.84361336047702 9 26.18296940955756 15.17275522434728 20.824556743698928 37.81971862239623 10 14.710712958869246 29.33833900134318 35.14811913411414 20.802828905673447 11 33.547487723981654 22.390810259237902 25.704116437676827 18.35758557910362 12 23.247105616625117 24.85404104165861 32.52552285499589 19.373330486720384 13 28.886257079408733 22.469526391968518 26.178640652658864 22.465575875963882 14 20.24563804195616 23.073493046251297 28.256948285223892 28.42392062656865 15 22.26061133814904 32.31471659670611 23.697002146727208 21.727669918417643 16 21.108735193945446 28.595101688133973 29.002762003705087 21.29340111421549 17 20.89558197825031 29.29194145167174 28.61308310036496 21.19939346971299 18 20.721785400135243 27.588883570161464 31.30506301291106 20.384268016792237 19 23.000408500165587 27.048090387806184 28.33406740084625 21.617433711181977 20 23.08774011992758 27.70459049959738 29.0651650222994 20.142504358175632 21 22.26422564002562 27.009089548951938 27.942041727535432 22.784643083487012 22 22.61695628712014 28.81191173034282 28.13124622809775 20.43988575443929 23 21.500725381981276 28.431569497981872 28.781358271455925 21.286346848580926 24 22.585736098788427 27.126385960281713 29.497538569003495 20.790339371926365 25 22.038209975410087 27.868343314010787 29.08590331049734 21.007543400081786 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 325.0 1 325.0 2 542.5 3 760.0 4 760.0 5 760.0 6 1862.0 7 2964.0 8 2964.0 9 2964.0 10 4264.5 11 5565.0 12 5565.0 13 5565.0 14 8903.5 15 12242.0 16 12242.0 17 12242.0 18 21958.0 19 31674.0 20 31674.0 21 31674.0 22 50598.5 23 69523.0 24 69523.0 25 69523.0 26 99981.5 27 130440.0 28 130440.0 29 130440.0 30 167599.0 31 204758.0 32 204758.0 33 204758.0 34 228517.5 35 252277.0 36 252277.0 37 252277.0 38 265856.5 39 279436.0 40 279436.0 41 279436.0 42 289775.5 43 300115.0 44 300115.0 45 300115.0 46 303523.0 47 306931.0 48 306931.0 49 306931.0 50 290553.5 51 274176.0 52 274176.0 53 274176.0 54 238989.0 55 203802.0 56 203802.0 57 203802.0 58 172100.5 59 140399.0 60 140399.0 61 140399.0 62 113902.0 63 87405.0 64 87405.0 65 87405.0 66 66336.5 67 45268.0 68 45268.0 69 45268.0 70 32763.0 71 20258.0 72 20258.0 73 20258.0 74 13910.5 75 7563.0 76 7563.0 77 7563.0 78 5077.0 79 2591.0 80 2591.0 81 2591.0 82 1662.0 83 733.0 84 733.0 85 733.0 86 457.5 87 182.0 88 182.0 89 182.0 90 110.0 91 38.0 92 38.0 93 38.0 94 23.5 95 9.0 96 9.0 97 9.0 98 5.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40837408528743785 2 0.0 3 0.0 4 0.0 5 0.026308755520215714 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.6810706402693747E-4 17 0.0 18 1.260802980202031E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0054214528148687335 25 2.1013383003367186E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2379436.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.7296712032098 #Duplication Level Percentage of deduplicated Percentage of total 1 82.64824078220767 57.63034655267057 2 12.038817065202304 16.789255112642955 3 2.958696062095973 6.189267108005513 4 1.0066591301916188 2.8077604064788297 5 0.45439361107308784 1.5842358548482802 6 0.22714663757062697 0.9503316211628688 7 0.1378512114605396 0.6728623755075301 8 0.09036457458659855 0.5040873659473156 9 0.07288492785799902 0.4574017849687144 >10 0.3198947873820994 3.9907087277688857 >50 0.022301949714207375 1.0854297079712882 >100 0.01852978941919047 2.733187315488186 >500 0.002109735619054673 1.046002457873904 >1k 0.0017480666557842712 2.0774198939295987 >5k 3.6166896326571133E-4 1.4817037147355836 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7970 0.3349533250736729 No Hit GTCCTACAGTGGACATTTCTAAATT 5955 0.2502693915701032 No Hit CTGTAGGACGTGGAATATGGCAAGA 5416 0.22761696469247336 No Hit GGTATCAACGCAGAGTACTTTTTTT 5391 0.226566295542305 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5335 0.22421279664592786 No Hit TATCAACGCAGAGTACTTTTTTTTT 5185 0.2179087817449177 No Hit CTTTAGGACGTGAAATATGGCGAGG 4981 0.2093353214795439 No Hit GTCCTACAGTGTGCATTTCTCATTT 3144 0.13213215232517284 No Hit CTGAAGGACCTGGAATATGGCGAGA 2723 0.11443888383633768 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2478 0.10414232616468777 No Hit CTGTAGGACCTGGAATATGGCGAGA 2456 0.10321773731253961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 4.202676600673437E-5 0.0 11 0.0 0.0 0.0 4.202676600673437E-5 0.0 12 0.0 0.0 0.0 8.405353201346874E-5 4.202676600673437E-5 13 0.0 0.0 0.0 8.405353201346874E-5 8.405353201346874E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1575 0.0 13.983081 1 TAAGTCG 50 0.0015008432 13.298956 5 TAGGACC 1000 0.0 12.3490305 4 TCCAACG 185 0.0 11.295697 18 TGTAGGA 3355 0.0 11.042353 2 GGCGAGG 1395 0.0 10.758332 19 TTAGGAC 1935 0.0 10.751093 3 CTGTAGG 3295 0.0 10.734701 1 GCCGTGC 90 9.542863E-5 10.552952 8 AGGACGT 3290 0.0 10.538686 5 GTAGGAC 3370 0.0 10.485823 3 GGACGTG 3300 0.0 10.418641 6 TAGGACT 430 0.0 10.382905 4 TAGGACA 925 0.0 10.166769 4 GTCCTAA 1565 0.0 10.112656 1 AGGACCT 1900 0.0 10.099206 5 GACGTGA 1745 0.0 10.069149 7 GACGTGG 1670 0.0 10.066379 7 TAGGACG 3555 0.0 9.966868 4 GCTATTC 115 1.0278318E-5 9.912265 3 >>END_MODULE