FastQCFastQC Report
Thu 26 May 2016
SRR1511795_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511795_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167955
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53530.24691471917083152No Hit
GTCCTACAGTGGACATTTCTAAATT39760.18339864065444164No Hit
GGTATCAACGCAGAGTACTTTTTTT35650.16444068257874356No Hit
CTGTAGGACGTGGAATATGGCAAGA33590.1549386403315567No Hit
TATCAACGCAGAGTACTTTTTTTTT31400.1448369546415862No Hit
GTCCTAAAGTGTGTATTTCTCATTT31150.1436837941746946No Hit
CTTTAGGACGTGAAATATGGCGAGG27610.12735504196350939No Hit
GTCCTACAGTGTGCATTTCTCATTT22220.10249290229732627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.013.5297791
TAGGACC8900.013.2657634
TCCAACG1302.1827873E-1012.3517318
AGGACCT15550.011.4800475
CCAACGA1105.355141E-711.16279419
GGCGAGG10900.011.09189519
GTATCAA24150.011.0890771
TGTAGGA23950.010.9730662
GGACCTG15250.010.9007176
GTCCTAA9850.010.7534381
GTAGGAC24950.010.7512773
CGGTAGG854.1395368E-410.5803811
GGACGTG21800.010.5717176
AGGACGT22750.010.5454365
GTCCTAC18800.010.5241031
TTAGGAC14300.010.4359813
GACGTGA11950.010.4329777
GTCCTAT2600.010.3769121
CTGTAGG23600.010.3736551
ATTTAGA5800.010.3371541
Produced by FastQC (version 0.11.5)